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The effect of eight weeks of high-intensity interval training on intracellular levels of FIP200, ATG13 and ULK1/2 proteins in fast-twitch muscle tissue (EDL) of aged rats
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Salman Nosrati    , Neda Aghaei Bahmanbegloo * , Hamed Alizadeh pahlavani |
| , nedaaghaei@aliabadiau.ac.ir |
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Abstract: (5 Views) |
Objective: The aim of the present study was to investigate the effect of eight weeks of high-intensity interval training (HIIT) on some molecular markers related to the initiation of the autophagy process, including FIP200, ATG13 and ULK1/ULK2 proteins in fast-twitch muscle tissue (EDL) of aged rats.
Methods: In this experimental study, the samples were divided into two HIIT and control groups (6 rats per group). The training group underwent a high-intensity interval training protocol for eight weeks, while the control group continued their normal activity. After the end of the training period, EDL muscle tissue was extracted and the intracellular levels of the target proteins were measured. The data were analyzed using independent t-test and Cohen′s d effect size.
Results: The results showed that HIIT training significantly increased the levels of FIP200 (t = 9.28; p = 0.001; Cohen′s d = 5.35) and ATG13 (t = 8.61; p = 0.001; Cohen′s d = 4.97) proteins compared to the control group. However, no significant difference was observed in the ULK1/ULK2 ratio between the two groups (t = 1.60; p = 0.17; Cohen′s d = 0.92)
Conclusion: Overall, the findings indicate that high-intensity interval training can enhance the autophagic capacity of aged skeletal muscle by increasing key components of the autophagy initiation complex, although the ULK1/ULK2 ratio was not affected. These results indicate the potential role of HIIT in improving cellular clearance mechanisms and counteracting age-related changes in skeletal muscle.
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| Keywords: High-intensity interval training, autophagy, FIP200, ATG13, ULK1/ULK2, aging, skeletal muscle. |
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Type of Study: Research |
Subject:
medicine, paraclinic
Received: 2026/06/8 | Revised: 2026/09/16 | Accepted: 2026/09/16
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