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Journal volumes: 30
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:: Volume 30, Issue 4 (Bimonthly 2026) ::
Feyz Med Sci J 2026, 30(4): 404-414 Back to browse issues page
The Effect of Eight Weeks of High-Intensity Interval Training on Intracellular FIP200 and ATG13 Protein Levels and the ULK1/ULK2 Ratio in Fast-Twitch Muscle (EDL) of Aged Rats
Salman Nosrati , Neda Aghaei Bahmanbegloo * , Hamed Alizadeh pahlavani
Department of Physical Education and Sports Sciences, AK. C, Islamic Azad University, Aliabad Katoul, Iran , nedaaghaei@aliabadiau.ac.ir
Abstract:   (245 Views)
Background and Aim: The aim of the present study was to investigate the effect of eight weeks of high-intensity interval training (HIIT) on some molecular markers related to the initiation of the autophagy process, including FIP200 and ATG13 protein levels and the ULK1/ULK2 ratio in fast-twitch muscle tissue (EDL) of aged rats.
Methods: In this experimental study, the rats were randomly divided into two groups: a HIIT group and a control group (6 rats per group). The training group underwent a high-intensity interval training protocol for eight weeks, while the control group continued their normal activity. After the training period, EDL muscle tissue was extracted, and the intracellular levels of the target proteins were measured. The data were analyzed using an independent samples t-test and Cohen's d effect size.
Results: The results showed that HIIT significantly increased the levels of FIP200 (t = 9.28; P=0.001; Cohen's d=5.35) and ATG13 (t=8.61; P=0.001; Cohen's d=4.97) proteins compared with the control group. However, no significant difference was observed in the ULK1/ULK2 ratio between the two groups (t =1.60; P=0.17; Cohen's d=0.92).
Conclusion: Overall, the findings indicate that high-intensity interval training can enhance the autophagic capacity of aged skeletal muscle by increasing key components of the autophagy initiation complex, although the ULK1/ULK2 ratio was not affected. These results suggest that HIIT may play a role in improving cellular clearance mechanisms and mitigating age-related changes in skeletal muscle.
Keywords: High-intensity interval training, autophagy, FIP200, ATG13, ULK1/ULK2, Aging, Skeletal muscle
Full-Text [PDF 895 kb]   (13 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2026/06/8 | Revised: 2026/10/6 | Accepted: 2026/09/16 | Published: 2026/10/4
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Nosrati S, Aghaei Bahmanbegloo N, alizadeh pahlavani H. The Effect of Eight Weeks of High-Intensity Interval Training on Intracellular FIP200 and ATG13 Protein Levels and the ULK1/ULK2 Ratio in Fast-Twitch Muscle (EDL) of Aged Rats. Feyz Med Sci J 2026; 30 (4) :404-414
URL: http://feyz.kaums.ac.ir/article-1-5546-en.html


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Volume 30, Issue 4 (Bimonthly 2026) Back to browse issues page
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