fa تاثیر هشت هفته تمرین تناوبی پرشدت بر میزان درون سلولی پروتئین‌ FIP200، ATG13 و نسبت ULK1/2 در بافت عضله تند انقباض (EDL) موش‌های صحرایی سالمند medicine, paraclinic medicine, paraclinic پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Aptos,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">هدف: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">هدف از پژوهش حاضر بررسی تأثیر هشت هفته تمرین تناوبی پرشدت (</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) بر برخی شاخص‌های مولکولی مرتبط با آغاز فرایند اتوفاژی شامل پروتئین‌های </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">FIP200</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">، </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ATG13</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و نسبت </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ULK1/ULK2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> در بافت عضله تند انقباض (</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">EDL</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) موش‌های صحرایی سالمند بود.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Aptos,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">روش بررسی: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">در این مطالعه تجربی، نمونه‌ها به دو گروه تمرین </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> و کنترل تقسیم شدند (هرگروه 6 رت). گروه تمرین به مدت هشت هفته تحت پروتکل تمرین تناوبی پرشدت قرار گرفتند، در حالی که گروه کنترل فعالیت عادی خود را ادامه دادند. پس از پایان دوره تمرینی، بافت عضله </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">EDL</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> استخراج و میزان درون‌سلولی پروتئین‌های مورد نظر اندازه‌گیری شد. داده‌ها با استفاده از آزمون </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">t</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> مستقل و اندازه اثر کوهن-</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">d</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Cohen</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&prime;</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">s d</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) تحلیل شدند. </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Lotus""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Aptos,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتایج: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتایج نشان داد که تمرین </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> موجب افزایش معنی‌دار در میزان پروتئین </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">FIP200</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">(35/5=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Choen&rsquo;d</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">؛ 001/</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">0=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> ؛ 28/9=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">t</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) و </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ATG13 </span></span><span style="font-size:12.0pt"><span b="" lotus="" style="font-family:">&nbsp;(97/4=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Choen&rsquo;d</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">؛ 001/</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">0=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> ؛ 61/8=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">t</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">) در مقایسه با گروه کنترل شد. با این حال، تفاوت معناداری در نسبت </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ULK1/ULK2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> بین دو گروه مشاهده نشد (92/0=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Choen&rsquo;d</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">؛ 17/</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">0=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">p</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> ؛ 60/1=</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">t</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Aptos,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">نتیجه‌گیری: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:">به طور کلی، یافته‌ها نشان داد که تمرین تناوبی پرشدت می‌تواند با افزایش اجزای کلیدی کمپلکس آغازگر اتوفاژی، ظرفیت اتوفاژیک عضله اسکلتی سالمند را تقویت کند، هرچند که نسبت </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">ULK1/ULK2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> تحت تأثیر قرار نگرفت. این نتایج بیانگر نقش بالقوه </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">HIIT</span></span><span lang="FA" style="font-size:12.0pt"><span b="" lotus="" style="font-family:"> در بهبود سازوکارهای پاکسازی سلولی و مقابله با تغییرات وابسته به سالمندی در عضله اسکلتی است.</span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Aptos,sans-serif"><b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Objective: </span></span></span></b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">The aim of the present study was to investigate the effect of eight weeks of high-intensity interval training (HIIT) on some molecular markers related to the initiation of the autophagy process, including FIP200, ATG13 and ULK1/ULK2 proteins in fast-twitch muscle tissue (EDL) of aged rats.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Aptos,sans-serif"><b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">In this experimental study, the samples were divided into two HIIT and control groups </span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">(6 rats per group)</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">. The training group underwent a high-intensity interval training protocol for eight weeks, while the control group continued their normal activity. After the end of the training period, EDL muscle tissue was extracted and the intracellular levels of the target proteins were measured. The data were analyzed using independent t-test and Cohen</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">&prime;</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">s d effect size.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Aptos,sans-serif"><b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results: </span></span></span></b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">The results showed that HIIT training significantly increased the levels of FIP200 (t = 9.28; p = 0.001; Cohen</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">&prime;</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">s d = 5.35) and ATG13 (t = 8.61; p = 0.001; Cohen</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">&prime;</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">s d = 4.97) proteins compared to the control group. However, no significant difference was observed in the ULK1/ULK2 ratio between the two groups (t = 1.60; p = 0.17; Cohen</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">&prime;</span></span></span><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">s d =</span></span></span> <span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">0.92)</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span style="font-family:Aptos,sans-serif"><b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></span></b><span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Overall, the findings indicate that high-intensity interval training can enhance the autophagic capacity of aged skeletal muscle by increasing key components of the autophagy initiation complex, although the ULK1/ULK2 ratio was not affected. These results indicate the potential role of HIIT in improving cellular clearance mechanisms and counteracting age-related changes in skeletal muscle.</span></span></span></span></span></span><br> &nbsp; تمرین تناوبی پرشدت, اتوفاژی, FIP200, ATG13, ULK1/ULK2, سالمندی, عضله اسکلتی. High-intensity interval training, autophagy, FIP200, ATG13, ULK1/ULK2, aging, skeletal muscle. 0 0 http://feyz.kaums.ac.ir/browse.php?a_code=A-10-4788-4&slc_lang=fa&sid=1 salman nosrati سلمان نصرتی Snosrati040@gmail.com 2900319475328460053833 0009-0006-4567-3980 No ﮔﺮوه ﺗﺮﺑﯿﺖ ﺑﺪﻧﯽ و ﻋﻠﻮم ورزﺷﯽ، واﺣﺪ ﻋﻠﯽ آﺑﺎد ﮐﺘﻮل، داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ، ﻋﻠﯽ آﺑﺎد ﮐﺘﻮل، ایﺮان neda Aghaei Bahmanbegloo ندا آقایی بهمن بگلو nedaaghaei@aliabadiau.ac.ir 2900319475328460053834 2900319475328460053834 Yes ﮔﺮوه ﺗﺮﺑﯿﺖ ﺑﺪﻧﯽ و ﻋﻠﻮم ورزﺷﯽ، واﺣﺪ ﻋﻠﯽ آﺑﺎد ﮐﺘﻮل، داﻧﺸﮕﺎه آزاد اﺳﻼﻣﯽ، ﻋﻠﯽ آﺑﺎد ﮐﺘﻮل، ایﺮان hamed alizadeh pahlavani حامد علیزاده پهلوانی ha.alizadeh@cfu.ac.ir 2900319475328460053835 2900319475328460053835 No گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، صندوق پستی 889- 14665 تهران، ایران