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Effect of Human Placental Extract on Osteogenic Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells
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Fateme Parvinifar    , Hanieh Jafary * , Ehsan Seyed jafari |
| , h-jafary@iau.ac.ir |
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Abstract: (2 Views) |
Background and Aim: Mesenchymal stem cells possess self-renewal capacity and the ability to differentiate into various cell lineages. Human placenta extract is rich in growth factors and amino acids and has been widely considered in regenerative medicine. This study aimed to investigate the effect of human placenta extract on the osteogenic differentiation of mesenchymal stem cells.
Methods: In this experimental study, the cytotoxicity and proliferative effects of human placenta extract on mesenchymal stem cells were evaluated using the MTT assay. Osteogenic differentiation was assessed through biochemical and molecular analyses, including alkaline phosphatase (ALP) activity, calcium deposition, and gene expression analysis using real-time PCR.. All experiments were performed in triplicate. Data were expressed as mean ± standard deviation (SD) and analyzed using one-way ANOVA. Real-time PCR data were analyzed using LinRegPCR and REST-RGXL software. A P value <0.05 was considered statistically significant.
Results: The MTT assay results indicated that placenta extract was non-toxic and even enhanced cell viability and proliferation. Furthermore, osteogenic differentiation was significantly improved. After 14 days of culture, ALP activity and calcium deposition increased progressively in treated groups compared to the control group. Osteocalcin expression also increased at higher concentrations of placenta extract, with statistically significant differences (P < 0.05).
Conclusion: Based on the findings, human placenta extract enhances and accelerates the osteogenic differentiation of mesenchymal stem cells. The observed increases in ALP activity, calcium deposition, and osteocalcin expression indicate its potential role in bone tissue engineering applications.
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| Keywords: Placenta extract, Mesenchymal stem cells, Differentiation, Umbilical cord, Bone |
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Type of Study: Research |
Subject:
medicine, paraclinic
Received: 2026/06/12 | Revised: 2026/07/22 | Accepted: 2026/07/22
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