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:: Volume 30, Issue 3 (Bimonthly 2026) ::
Feyz Med Sci J 2026, 30(3): 266-280 Back to browse issues page
Effect of Human Placental Extract on Osteogenic Differentiation of Umbilical Cord-Derived Mesenchymal Stem Cells
Fateme Parvinifar , Hanieh Jafary * , Ehsan Seyed jafari
Departement of Biology, SR.C., Islamic Azad University, Tehran, Iran , h-jafary@iau.ac.ir
Abstract:   (317 Views)
Background and Aim: Mesenchymal stem cells (MSCs) are characterized by self-renewal capacity and multilineage differentiation potential. Human placental extract, rich in growth factors and amino acids, has garnered increasing attention in regenerative medicine. This study aimed to evaluate the effect of human placental extract on the osteogenic differentiation of MSCs.
Methods: In this experimental study, the cytotoxicity and proliferative effects of placental extract on MSCs were assessed using the MTT assay. Osteogenic differentiation was evaluated through biochemical and molecular analyses, including alkaline phosphatase (ALP) activity, calcium deposition measurement, and real-time PCR-based gene expression analysis. All experiments were performed in triplicate. Data were expressed as mean ± standard deviation (SD) and analyzed using one-way ANOVA. Real-time PCR data were processed with LinRegPCR and REST-RGXL software, with statistical significance set at P<0.05.

Results: The MTT assay revealed that placental extract was non‑toxic and significantly enhanced cell viability and proliferation. Moreover, osteogenic differentiation was markedly improved in treated groups. After 14 days of culture, ALP activity and calcium deposition progressively increased compared to the control group. Osteocalcin gene expression also showed a significant upregulation at higher concentrations of the extract (P<0.05), confirming the osteoinductive effect.
Conclusion: Findings of the present study demonstrate that human placental extract effectively enhances and accelerates the osteogenic differentiation of MSCs. The significant increases in ALP activity, calcium deposition, and osteocalcin expression support its potential as a promising biological supplement for bone tissue engineering and regenerative therapeutic strategies.
Keywords: Placenta extract, Mesenchymal stem cells, Differentiation, Umbilical cord, Bone
Full-Text [PDF 1595 kb]   (32 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2026/06/12 | Revised: 2026/08/25 | Accepted: 2026/07/22 | Published: 2026/08/23
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Parvinifar F, Jafary H, Seyed jafari E. Effect of Human Placental Extract on Osteogenic Differentiation of Umbilical Cord-Derived Mesenchymal Stem Cells. Feyz Med Sci J 2026; 30 (3) :266-280
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Volume 30, Issue 3 (Bimonthly 2026) Back to browse issues page
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