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The Effect of Aerobic Training along with Aqueous Extract of Garlic (Allium sativum L.) on Cardiac Mitochondrial Biogenesis Markers in diabetic rats
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Masoumeh Ebrahimpour    , Saqqa Farajtabar Behrestaq * |
| Qaemshahr Branch, Islamic Azad University , farajtabarp@yahoo.com |
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Abstract: (88 Views) |
Background and Aim: Exercise training causes adaptations such as increasing antioxidant capacity, mitochondrial size and improving insulin signaling, and ultimately improves muscle function and protects against increased metabolic disorders. The purpose of this research was to determine the effect of consuming garlic aqueous extract with aerobic exercise on the markers of mitochondrial biogenesis in the heart of diabetic rats.
Methods: For this experimental research, 40 male Wistar rats (age 5 weeks and weight 162.12 ± 15.24 grams) were obtained from Pasteur Institute. 8 rats were included in the healthy control group (CN). 32 rats were made diabetic by intraperitoneal injection of a single dose of streptozotocin (60 mg/kg) and randomly divided into four groups: diabetes (DM), and diabetes-exercise. (TDM), diabetes-garlic (GDM) and diabetes-exercise-garlic (TGDM) were divided. In the following, the training groups performed the aerobic exercise program on the treadmill for eight weeks. Also, one milliliter (0.4 grams per 100 grams of body weight) of garlic extract was gavage daily for every hundred grams of body weight. Changes in PGC-1α and Nrf2 gene expression were measured in heart tissue. Finally, independent t-test, two-way analysis of variance and Benferroni's post hoc test were used to compare the average changes of the research variables at a significance level of p≤0.05.
Results: The expression of PGC-1α and Nrf2 was significantly increased in TDM (p=0.038 and p=0.031) and GDM (p=0.036 and p=0.025, respectively) groups compared to DM. The combined intervention of training with supplementation was not significant compared to the effect of each intervention alone on PGC-1α and Nrf2 (p<0.05).
Conclusion: Garlic supplementation and exercise activity can inhibit STZ-induced cardiac damage possibly through up-regulation of PGC-1α and Nrf2 pathways. |
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| Keywords: Exercise, Garlic, PGC-1α, Nrf2, Diabetes and Rat. |
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Type of Study: Research |
Subject:
medicine, paraclinic
Received: 2024/05/30 | Revised: 2024/07/8 | Accepted: 2024/07/7
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