Impact of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation on visfatin serum levels and insulin resistance index in obese mice

Hasanpour, Kimia; Habibi, Abdolhamid; Tabandeh, Mohamadrea; Rami, Mohammad

doi:10.48307/FMSJ.2023.27.6.629

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Volume 27, Issue 6 (Bimonthly 2023)

Feyz Med Sci J 2023, 27(6): 629-636

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Impact of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation on visfatin serum levels and insulin resistance index in obese mice

Kimia Hasanpour

, Abdolhamid Habibi ^*

, Mohamadrea Tabandeh

, Mohammad Rami

Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran , Hamidhabibi330@gmail.com

Abstract: (414 Views)

Background and Aim: Incorporating physical activity and a balanced diet can play a crucial role in managing and preventing obesity-related diseases. This study aimed to assess the effects of six weeks of High-Intensity Interval Training (HIIT) exercises combined with EPA/DHA and clenbuterol supplementation on visfatin serum levels and the insulin resistance index in obese mice.
Methods: In this experimental study, 35 male NMRI mice were divided into seven groups of five: healthy control, high-fat diet, high-fat diet with HIIT, high-fat diet with EPA/DHA supplement, high-fat diet with clenbuterol supplement, high-fat diet with clenbuterol supplement and HIIT, and high-fat diet with EPA/DHA supplement and HIIT. HIIT training was conducted at 90% of maximum speed for six weeks, with five sessions per week. The EPA/DHA supplement dosage for five days of training was 180 mg, while each rat received two clenbuterol tablets in 25 cc of distilled water for the same duration. Blood samples were collected directly from the heart 48 hours after the final exercise session, centrifuged, and stored at -20°C for subsequent analysis. A specific animal kit was utilized to measure visfatin levels and the insulin resistance index.
Results: The combination of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation led to a significant reduction in visfatin levels in obese mice (P=0.001). Additionally, this regimen resulted in a notable decrease in the insulin resistance index among obese mice (P=0.001).
Conclusion: Engaging in HIIT with appropriate intensity and duration can effectively reduce visfatin levels and improve insulin resistance. Furthermore, combining HIIT with the intake of supplements presents a promising approach to managing visfatin levels and insulin resistance.

Keywords: Interval training, EPA/DHA, Clenbuterol, Visfatin, Insulin resistance index, Obesity

Full-Text [PDF 443 kb] (246 Downloads)

Type of Study: Research | Subject: General
Received: 2023/06/27 | Revised: 2024/02/19 | Accepted: 2023/12/24 | Published: 2024/02/13

References

1. Armstrong A, Jungbluth Rodriguez K, Sabag A, Mavros Y, Parker HM, Keating SE, et al. Effect of aerobic exercise on waist circumference in adults with overweight or obesity: A systematic review and meta‐analysis. Obes Rev. 2022; 23(8): e13446. doi:10.1111/obr.13446 PMid:35383401 PMCid:PMC9540641

2. Mthembu SX, Mazibuko-Mbeje SE, Ziqubu K, Nyawo TA, Obonye N, Nyambuya TM, et al. Impact of physical exercise and caloric restriction in patients with type 2 diabetes: Skeletal muscle insulin resistance and mitochondrial dysfunction as ideal therapeutic targets. Life Sci. 2022; 297, 120467. doi:10.1016/j.lfs.2022.120467 PMid:35271881

3. Calcaterra V, Verduci E, Vandoni M, Rossi V, Fiore G, Massini G, Zuccotti G. The effect of healthy lifestyle strategies on the management of insulin resistance in children and adolescents with obesity: a narrative review. Nutrients. 2022; 14(21): 4692. doi:10.3390/nu14214692 PMid:36364954 PMCid:PMC9657567

4. Abdalla MMI. Role of visfatin in obesity-induced insulin resistance. World J Clin Cases. 2022; 10(30): 10840.‌ doi:10.12998/wjcc.v10.i30.10840 PMid:36338223 PMCid:PMC9631142

5. Ghorbani H, Dunyaei A, Qadiri. The effect of 8 weeks of combined exercises with the consumption of omega-3 and L-carnitine supplements on visfatin serum levels in type 2 diabetic women. Iran J Endocr Metab. 2017; 19: 18-25.

6. Sandeep S, Velmurugan K, Deepa R, Mohan V. Serum visfatin in relation to visceral fat, obesity, and type 2 diabetes mellitus in Asian Indians. Metabolism. 2007; 56(4): 565-70. doi:10.1016/j.metabol.2006.12.005 PMid:17379018

7. Van Baak MA, Battista F, Beaulieu K, Blundell JE, Busetto L, Oppert JM. Effect of exercise training on weight loss, body composition changes, and weight maintenance in adults with overweight or obesity: An overview of 12 systematic reviews and 149 studies. Obes Rev. 2021; 22: e13256. doi:10.1111/obr.13256 PMid:33955140 PMCid:PMC8365736

8. Liu Q. American ginseng and asian ginseng intervention in diet-induced obese mice: metabolomics reveals distinct metabolic profiles. Am J Chinese Med. 2019; 47: 787-801 doi:10.1142/S0192415X19500411 PMid:31091973

9. Borja-Magno AI, Furuzawa-Carballeda J, Guevara-Cruz M, Arias C, Granados J, Bourges, H, et al. Supplementation with EPA and DHA omega-3 fatty acids improves peripheral immune cell mitochondrial dysfunction and inflammation in subjects with obesity. J Nutr Biochem. 2023; 120: 109415.doi:10.1016/j.jnutbio.2023.109415 PMid:37437746

10. Ghod Elahi M, Hekmati M, Esmaeili D, Ziarati P, Yousefi M. Antibacterial activity of modified carvacrol against Staphylococcus epidermidis and Pseudomonas aeruginosa. Novel Clin Med 2022; 1(4): 192-196 doi: 10.22034/ncm.2022.350997.1051

11. Ghorbani M, Hassani A, Donyai A, Qadiri M. The effect of 8-weeks compound exercises training with omega-3 and l-carnitine supplementation intake on serum levels of visfatin in type 2 diabetic women, 2017.

12. Pan SJ, Hancock J, Ding Z, Fogt D, Lee M, Ivy JL. Effects of clenbuterol on insulin resistance in conscious obese Zucker rats. Am J Physiol Endocr Metab. 2001; 280(4): E554-E561 doi:10.1152/ajpendo.2001.280.4.E554 PMid:11254461

13. Ghahri S, Dianatinasab A, Fararouei M, Dianatinasab M. Exercise and type 2 diabetes. Physical Exerc Human Health. 2020; 91-105 doi:10.1007/978-981-15-1792-1_6 PMid:32342452

14. Gibala MJ, Little JP, Macdonald MJ, Hawley JA. Physiological adaptations to low-volume, high-intensity interval training in health and disease. J Physiol 2012; 590(Pt 5): 1077-84 doi:10.1113/jphysiol.2011.224725 PMid:22289907 PMCid:PMC3381816

15. Von Schacky C. (Importance of EPA and DHA blood levels in brain structure and function. Nutrients. 2021; 13(4): 1074 doi:10.3390/nu13041074 PMid:33806218 PMCid:PMC8066148

16. Bone DB, Knudsen JR, Barella LF, Velenosi TJ, Akhmedov D, Wess J. Clenbuterol exerts antidiabetic activity through metabolic reprogramming of skeletal muscle cells. Nature Commun. 2022; 13(1): 22 doi:10.1038/s41467-021-27540-w PMid:35013148 PMCid:PMC8748640

17. Hoseinzadeh Attar M, Hajianfar H, Bahonar A, Mohamad K, Keshavarz S, Entezari M, Askari G. The effect of n-fatty acid (omega 3) on serum visfatin concentration in patients with type2 diabetes. Pars J Med Sci. 2022; 10(1): 26-32. doi:10.29252/jmj.10.1.26

18. Berndt J, Kloting N, Kralisch S, Kovacs P, Fasshauer M, Schön MR. Plasma visfatin concentrations and fat depot-specific mRNA expression in humans. Diabetes. 2005; 54(10): 2911-6. doi:10.2337/diabetes.54.10.2911 PMid:16186392

19. 19. Lee JK. Effects of exercise type and intensity on visfatin and the metabolic syndrome in obesity. Revista Brasileira de Medicina do Esporte. 2021; 27: 170-3. doi:10.1590/1517-869220212706000539

20. Dehghani-Firouzabadi R, Daneshbodi H, Lotfi MH, Vaziri N, Mozaffari-Khosravi H. Effect of omega-3 supplementation on visfatin, adiponectin, and anthropometric indices in women with polycystic ovarian syndrome. J Reproduction Infertil 2015; 16(4): 212.

21. Nayebifar SH, Ghasemi E, Karimipour S. Effect of high-intensity interval training and omega-3 supplementation on liver enzymes and lipid profile of young men. Sci Sports. 2020; 35(1): e1-e9. doi:10.1016/j.scispo.2019.03.004

22. Kuo CH, Ding Z, Ivy JL. Interaction of exercise training and clenbuterol on GLUT-4 protein in muscle of obese Zucker rats. Am J Physiol Endocrinol Metab. 1996; 271: E847-E854 doi:10.1152/ajpendo.1996.271.5.E847 PMid:8944671

23. Rothwell NJ, Stock MJ, Sudera DK. Changes in tissue blood flow and b-receptor density of skeletal muscle in rats treated with the b-adrenoceptor agonist, clenbuterol. Br J Pharmaco. 1987; l90: 601-7. doi:10.1111/j.1476-5381.1987.tb11211.x PMid:3032321 PMCid:PMC1917183

24. Bahram ME, Afroonde R, Pourvaghar MJ, Ghiyami Taklimi H, Hemmati S. Effect of resistance training on serum visfatin, insulin resistance, glucose and some body composition indices in obese adolescents. Sport Physiol Management Investigations. 2022; 14(1): 147-59.

25. Choi K, Kim J, Cho G, Baik S, Park H, Kim S. Effect of exercise training on plasma visfatin and eotaxin levels. Eur J Endocrinol. 2007; 157: 437-42. doi:10.1530/EJE-07-0127 PMid:17893257

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Hasanpour K, Habibi A, Tabandeh M, Rami M. Impact of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation on visfatin serum levels and insulin resistance index in obese mice. Feyz Med Sci J 2023; 27 (6) :629-636
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