Impact of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation on visfatin serum levels and insulin resistance index in obese mice

Hasanpour, Kimia; Habibi, Abdolhamid; Tabandeh, Mohamadrea; Rami, Mohammad

doi:10.48307/FMSJ.2023.27.6.629

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Volume 27, Issue 6 (Bimonthly 2023)

Feyz Med Sci J 2023, 27(6): 629-636

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Impact of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation on visfatin serum levels and insulin resistance index in obese mice

Kimia Hasanpour

, Abdolhamid Habibi ^*

, Mohamadrea Tabandeh

, Mohammad Rami

Department of Sport Physiology, Faculty of Sport Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran , Hamidhabibi330@gmail.com

Abstract: (692 Views)

Background and Aim: Incorporating physical activity and a balanced diet can play a crucial role in managing and preventing obesity-related diseases. This study aimed to assess the effects of six weeks of High-Intensity Interval Training (HIIT) exercises combined with EPA/DHA and clenbuterol supplementation on visfatin serum levels and the insulin resistance index in obese mice.
Methods: In this experimental study, 35 male NMRI mice were divided into seven groups of five: healthy control, high-fat diet, high-fat diet with HIIT, high-fat diet with EPA/DHA supplement, high-fat diet with clenbuterol supplement, high-fat diet with clenbuterol supplement and HIIT, and high-fat diet with EPA/DHA supplement and HIIT. HIIT training was conducted at 90% of maximum speed for six weeks, with five sessions per week. The EPA/DHA supplement dosage for five days of training was 180 mg, while each rat received two clenbuterol tablets in 25 cc of distilled water for the same duration. Blood samples were collected directly from the heart 48 hours after the final exercise session, centrifuged, and stored at -20°C for subsequent analysis. A specific animal kit was utilized to measure visfatin levels and the insulin resistance index.
Results: The combination of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation led to a significant reduction in visfatin levels in obese mice (P=0.001). Additionally, this regimen resulted in a notable decrease in the insulin resistance index among obese mice (P=0.001).
Conclusion: Engaging in HIIT with appropriate intensity and duration can effectively reduce visfatin levels and improve insulin resistance. Furthermore, combining HIIT with the intake of supplements presents a promising approach to managing visfatin levels and insulin resistance.

Keywords: Interval training, EPA/DHA, Clenbuterol, Visfatin, Insulin resistance index, Obesity

Full-Text [PDF 443 kb] (371 Downloads)

Type of Study: Research | Subject: General
Received: 2023/06/27 | Revised: 2024/02/19 | Accepted: 2023/12/24 | Published: 2024/02/13

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Hasanpour K, Habibi A, Tabandeh M, Rami M. Impact of six weeks of HIIT exercises with EPA/DHA and clenbuterol supplementation on visfatin serum levels and insulin resistance index in obese mice. Feyz Med Sci J 2023; 27 (6) :629-636
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