[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 25, Issue 6 (Bimonthly 2021) ::
Feyz 2021, 25(6): 1275-1285 Back to browse issues page
Investigating the synergistic effect of berberine and cisplatin on induction of apoptosis in Calu-6 cell line and evaluation of changes in the expression level of BAX, p53, Caspase 3, Caspase 9 genes
Shadab Abhari , Javad Baharara , Saeedeh Zafar-Balanezhad
azad Department of Biology and Research Center, Mashhad Branch, Islamic Azad University, Mashhad, I.R. Iran. , baharara78@gmail.com
Abstract:   (1487 Views)
Background: Lung cancer is the most common cause of cancer death worldwide. Berberine as a herbal alkaloid has antioxidant, anti-cancer and anti-tumor properties. Cisplatin is a potent inducer of apoptosis in most types of cancer cells. In the present study, investigated the ability of berberine alone and in combination with cisplatin to induce apoptosis in the Calu-6 cell line (human lung cancer cell line).
Materials and Methods: In this experimental study, MTT assay was used to evaluate the cytotoxic effects of combination of berberine with cisplatin on Calu-6 cell proliferation; DAPI staining and annexin V-FITC assay were also used to evaluate apoptosis in treated cells; changes in the level of transcripts of BAX, Caspase 3,9, p53 genes were examined by real-time PCR. Quantitative data were analyzed by one-way ANOVA at a significant level of P<0.05.
Results: The MTT assay results showed that the combination of berberine with cisplatin inhibits the proliferation of Calu-6 cells in a concentration- and time-dependent manner. Morphological observations obtained from DAPI staining method and annexin V-FITC assay showed an increase in apoptotic cells in the treatment groups. The real-time PCR results showed an increase in the expression of transcripts of BAX, Caspase 3,9, p53 genes in the treatment groups.
Conclusion: The results of this research showed that the concomitant use of berberine with cisplatin inhibits the proliferation of Calu-6 cells and induces apoptosis in these cells.
Keywords: Lung cancer, Apoptosis, Berberine, Cisplatin, Calu-6 cell line
Full-Text [PDF 657 kb]   (794 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2021/07/12 | Revised: 2023/11/1 | Accepted: 2021/12/18 | Published: 2022/02/1
References
1. Liu T, Liu X, Li W. Tetrandrine, a Chinese plant-derived alkaloid, is a potential candidate for cancer chemotherapy. Oncotarget 2016; 7(26): 40800–15.
2. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin 2021; 71(3): 209–49.
3. Farhood B, Geraily G, Alizadeh A. Incidence and mortality of various cancers in Iran and compare to other countries: A review article. Iran J Public Health 2018; 47(3): 309–16.
4. Fitzmaurice C, Akinyemiju TF, Al Lami FH, Alam T, Alizadeh-Navaei R, Allen C, et al. Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 29 cancer groups, 1990 to 2016 a systematic analysis for the global burden of disease study. JAMA Oncol 2018; 4(11): 1553–68.
5. Carvalho Â, Ferreira G, Seixas D, Guimarães-Teixeira C, Henrique R, Monteiro FJ, et al. Emerging lab-on-a-chip approaches for liquid biopsy in lung cancer: Status in ctcs and ctdna research and clinical validation. Cancers 2021; 13(9): 1-23.
6. Hirsch FR, Scagliotti G V., Mulshine JL, Kwon R, Curran WJ, Wu YL, et al. Lung cancer: current therapies and new targeted treatments. Lancet 2017; 389(10066): 299–311.
7. Lemjabbar-Alaoui H, Hassan OUI, Yang YW, Buchanan P. Lung cancer: Biology and treatment options. Biochim Biophys Acta - Rev Cancer 2015; 1856(2): 189–210.
8. Huang CY, Ju DT, Chang CF, Muralidhar Reddy P, Velmurugan BK. A review on the effects of current chemotherapy drugs and natural agents in treating non-small cell lung cancer. Biomed 2017; 7(4): 12–23.
9. Fennell DA, Summers Y, Cadranel J, Benepal T, Christoph DC, Lal R, et al. Cisplatin in the modern era: The backbone of first-line chemotherapy for non-small cell lung cancer. Cancer Treat Rev 2016; 44: 42–50.
10. Baharara J, Amini E, Nikdel N, Salek-Abdollahi F. The cytotoxicity of dacarbazine potentiated by sea cucumber saponin in resistant B16F10 melanoma cells through apoptosis induction. Avicenna J Med Biotechnol 2016; 8(3): 112–9.
11. Goldar S, Khaniani MS, Derakhshan SM, Baradaran B. Molecular mechanisms of apoptosis and roles in cancer development and treatment. Asian Pac J Cancer Prev 2015; 16(6): 2129–44.
12. Campbell KJ, Tait SWG. Targeting BCL-2 regulated apoptosis in cancer. Open Biol 2018; 8(5): 1–11.
13. Khalid EB, Ayman ELMELK, Rahman H, Abdelkarim G, Najda A. Natural products against cancer angiogenesis. Tumour Biol 2016; 37(11): 14513–36.
14. Zyad A, Tilaoui M, Jaafari A, Oukerrou MA, Mouse HA. More insights into the pharmacological effects of artemisinin. Phytother Res 2018; 32(2): 216–29.
15. Kooti W, Servatyari K, Behzadifar M, Asadi-Samani M, Sadeghi F, Nouri B, et al. Effective Medicinal Plant in Cancer Treatment, Part 2: Review Study. J Evid Based Complementary Altern Med 2017; 22(4): 982–95.
16. Kaur R, Arora S. Alkaloids- Important Therapeutic Secondary Metabolites of Plant Origin. J Crit Rev 2015; 2(3): 1–8.
17. Ortiz LMG, Lombardi P, Tillhon M, Scovassi AI. Berberine, an epiphany against cancer. Molecules 2014; 19(8): 12349–67.
18. Park KS, Kim J Bin, Bae J, Park SY, Jee HG, Lee KE, et al. Berberine inhibited the growth of thyroid cancer cell lines 8505C and TPC1. Yonsei Med J 2012; 53(2): 346–51.
19. Lin JF, Lin YC, Tsai TF, Chen HE, Chou KY, Hwang TIS. Cisplatin induces protective autophagy through activation of BECN1 in human bladder cancer cells. Drug Des Devel Ther 2017; 11: 1517–33.
20. Liu L, Sun L, Zheng J, Cui L. Berberine modulates Keratin 17 to inhibit cervical cancer cell viability and metastasis. J Recept Signal Transduct Res 2020; 0(0): 1–11.
21. Sani TA, Mohammadpour E, Mohammadi A, Memariani T, Yazdi MV, Rezaee R, et al. Cytotoxic and apoptogenic properties of Dracocephalum Kotschyi aerial part different fractions on calu-6 and mehr-80 lung cancer cell lines. Farmacia 2017; 65(2): 189–99.
22. Baharara J, Amini E, Afzali M, Nikdel N, Mostafapour A, Kerachian MA. Apoptosis inducing capacity of holothuria arenicola in CT26 colon carcinoma cells in vitro and in vivo. Iran J Basic Med Sci 2016; 19(4): 358–65.
23. Yu XJ, Sun K, Tang XH, Zhou CJ, Sun H, Yan Z, et al. Harmine combined with paclitaxel inhibits tumor proliferation and induces apoptosis through down-regulation of cyclooxygenase-2 expression in gastric cancer. Oncol Lett 2016; 12(2): 983–8.
24. Kumar S, Agnihotri N. Piperlongumine, a piper alkaloid targets Ras/PI3K/Akt/mTOR signaling axis to inhibit tumor cell growth and proliferation in DMH/DSS induced experimental colon cancer. Biomed Pharmacother 2019; 109: 1462–77.
25. Mousavi B, Tafvizi F, Zaker Bostanabad S. Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif Cells Nanomed Biotechnol 2018; 46(Sup1): 499–510.
26. Wu LW, Zhang JK, Rao M, Zhang ZY, Zhu HJ, Zhang C. Harmine suppresses the proliferation of pancreatic cancer cells and sensitizes pancreatic cancer to gemcitabine treatment. Onco Targets Ther 2019; 12: 4585–93.
27. Wang Y, Zhou M, Shang D. Berberine inhibits human gastric cancer cell growth via deactivation of p38/JNK pathway, induction of mitochondrial-mediated apoptosis, caspase activation and NF-κB inhibition. J BUON 2020; 25(1): 314–8.
28. Majidzadeh H, Araj-Khodaei M, Ghaffari M, Jafari A, Shanehbandi D, Torbati M, et al. The combination of berberine and methotrexate enhances anti-cancer effects in HeLa cancer cell line: A morphological study. Pharm Sci 2021; 27(4): 536-42.
29. Liu L, Fan J, Ai G, Liu J, Luo N, Li C, et al. Berberine in combination with cisplatin induces necroptosis and apoptosis in ovarian cancer cells. Biol Res 2019; 52(1): 1-14.
30. Mohiuddin M, Kasahara K. Cisplatin Activates the Growth Inhibitory Signaling Pathways by Enhancing the Production of Reactive Oxygen Species in Non-small Cell Lung Cancer Carrying an EGFR Exon 19 Deletion. Cancer Genomics Proteomics 2021; 18(3 Suppl): 471–86.
31. Zhu T, Li LL, Xiao GF, Luo QZ, Liu QZ, Yao KT, et al. Berberine Increases Doxorubicin Sensitivity by Suppressing STAT3 in Lung Cancer. Am J Chin Med 2015; 43(7):1487–502.
32. Chen J, Huang X, Tao C, Wang L, Chen Z, Li X, et al. Berberine chloride suppresses non-small cell lung cancer by deregulating Sin3A/TOP2B pathway in vitro and in vivo. Cancer Chemother Pharmacol 2020; 86(1): 151–61.
33. Kou Y, Tong B, Wu W, Liao X, Zhao M. Berberine Improves Chemo-Sensitivity to Cisplatin by Enhancing Cell Apoptosis and Repressing PI3K/AKT/mTOR Signaling Pathway in Gastric Cancer. Front Pharmacol 2020; 11(December):1–10.
34. Li Q, Zhao H, Chen W, Huang P. Berberine Induces Apoptosis and Arrests Cell Cycle in Multiple Cancer Cell lines. Arch Med Sci 2021; Articles in press.
35. Wang HY, Yu HZ, Huang SM, Zheng YL. P53, Bcl-2 and cox-2 are involved in berberine hydrochloride-induced apoptosis of HeLa229 cells. Mol Med Rep 2016; 14(4): 3855–61.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abhari S, Baharara J, Zafar-Balanezhad S. Investigating the synergistic effect of berberine and cisplatin on induction of apoptosis in Calu-6 cell line and evaluation of changes in the expression level of BAX, p53, Caspase 3, Caspase 9 genes. Feyz 2021; 25 (6) :1275-1285
URL: http://feyz.kaums.ac.ir/article-1-4432-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 25, Issue 6 (Bimonthly 2021) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.06 seconds with 46 queries by YEKTAWEB 4645