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:: Volume 22, Issue 5 (Bimonthly 2018) ::
Feyz Med Sci J 2018, 22(5): 487-496 Back to browse issues page
Antibacterial and anticancer activities of silver nanoparticles fabricated by the Artemisia scoparia extract against lung cancer cell line (A549)
Somaye Moulaie , Amir Mirzaie * , Elahe Aliasgari
Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, I. R. Iran. , Amir_mirzaie92@yahoo.com
Abstract:   (3806 Views)
Background: In recent years, due to an increase in prevalence of cancer, there is a need to find new ways to control this disease. The aim of this study was to evaluate antibacterial and anti-cancer activities of silver nanoparticles (AgNPs) fabricated by Artemisia scoparia extract gainst lung cancer cell line (A549).
Materials and Methods: In this experimental study, AgNPs was synthesized and ccharacterization of fabricated AgNPs was performed by scanning and transmission electron microscopy (SEM, TEM) methods. Subsequently, antibacterial activity of synthesized AgNPs was determined using the minimum inhibitory concentration (MIC) method. Moreover, cell toxicity of AgNPs against A549 cell line in 3.125-100 µg/mL concentrations was performed using the MTT method. The gene expression of Bax and Bcl2 was measured using the real-time PCR. 
Results: The results of SEM and TEM showed that the synthesized AgNPs had 33.40 nm average size. The MIC results demonstrated that the AgNPs had a significant antibacterial activity. In addition, the MTT results showed that AgNPs had dose-dependent cell toxicity. The real-time PCR results revealed that the Bax and Bcl2 gene expression were up-regulated (2.34±0.34) and down-regulated (0.43±0.61), respectively (P<0.05). 
Conclusion: According to the results of this study, the AgNPs had antibacterial and anticancer activities and it can be used as a drug candidate.
Keywords: Green synthesis, Silver nanoparticle, Lung cancer, Artemisia scoparia
Full-Text [PDF 493 kb]   (2928 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/02/20 | Revised: 2018/12/5 | Accepted: 2018/08/8 | Published: 2018/11/28
References
1. Fakruddin M, Hossain Z, Afroz H. Pros‌pects-and applications of nanobiotechnology:a medical perspective. J Nanobiotechnol 2012; 10: 31.
2. Marin S, Vlasceanu GM, Tiplea RE, Bucur IR, Lemnaru M, Marin MM, Grumezescu AM. Appl‌ic‌ations and toxicity of silver nanoparticles: a recent review. Curr Top Med Chem 2015; 15(16): 1596-604.
3. Rónavári A, Kovács D, Igaz N, Vágvölgyi C, Boros IM, Kónya Z, et al. Biological activity of green-synthesized silver nanoparticles depends on the applied natural extracts: a comprehensive study. Int J Nanomedicine 2017; 12: 871-83.
4. Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeutic Appro-aches. Int J Mol Sci 2016; 17(9).
5. Ivask A, Kurvet I, Kasemets K, Blinova I, Aruoja V, et al. Size-dependent toxicity of silver nanop‌arti‌cles to bacteria, yeast, algae, crustaceans and mam‌malian cells in vitro. PLoS One 2014; 9(7): 9(7): e102108.
6. Zhang W, Xiao B, Fang T. Chemical trans-formation of silver nanoparticles in aquatic environ‌ments: Mechanism, morphology and toxicity. Chemosphere 2018; 191: 324-34.
7. Kim TH, Kim M, Park HS, Shin US, Gong MS, Kim HW. Size-dependent cellular toxicity of silver nanoparticles. J Biomed Mater Res A 2012; 100(4): 1033-43.
8. Riaz Ahmed KB, Nagy AM, Brown RP, Zhang Q, Malghan SG. Silver nanoparticles: Significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies. Toxicol In Vitro 2017; 38: 179-92.
9. Siegel RL, Miller KD, Jemal A. Cancer statistics. CA Cancer J Clin 2018; 68(1): 7-30.
10. Azar FE, Azami-Aghdash S, Pournaghi-Azar F, Mazdaki A, Rezapour A, Ebrahimi P, et al. Cost-effectiveness of lung cancer screening and treatment methods: a systematic review of systematic reviews. BMC Health Serv Res 2017; 17(1): 413.
11. Bharali DJ, Khalil M, Gurbuz M, Simone TM, Mousa SA. Nanoparticles and cancer therapy: a concise review with emphasis on dendrimers. Int J Nanomedicine 2009; 4: 1-7.
12. Ong C, Lim JZ, Ng CT, Li JJ, Yung LY, Bay BH. Silver nanoparticles in cancer: therapeutic effi-ca‌cy and toxicity. Curr Med Chem 2013; 20(6): 772-81.
13. Durán N, Nakazato G, Seabra AB. Anti-microbial activity of biogenic silver nano‌particles, and silver chloride nanoparticles: an over‌view and comments. Appl Microbiol Biot‌ech‌nol 2016; 100(15): 6555-70.
14. Aboelfetoh EF, El-Shenody RA, Ghobara MM. Eco-friendly synthesis of silver nanoparticles using green algae (Caulerpa serrulata): reaction optim-ization, catalytic and antibacterial activities. Environ Monit Assess 2017; 189(7): 349.
15. Mashwani ZU, Khan MA, Khan T, Nadhman A. Applications of plant terpenoids in the synthesis of colloidal silver nanoparticles. Adv Colloid Interface Sci 2016; 234: 132-41.
16. Patil MP, Kim GD. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Appl Microbiol Bio-tech‌nol 2017; 101(1): 79-92.
17. Rajeshkumar S, Bharath LV. Mechanism of plant-mediated synthesis of silver nanoparticles- A review on biomolecules involved, characterisation and antibacterial activity. Chem Biol Interact 2017; 273: 219-27.
18. Meva FE, Segnou ML, Ebongue CO, Ntoumba AA, Steve DY, Malolo FA, et al. Unexplored vegetal green synthesis of silver nanoparticles: a preliminary study with Corchorus olitorus Linn and Ipomea batatas (L.) Lam. Afr J Biotechnol 2016; 15(10): 341–9.
19. Udayasoorian C, Kumar R, Jayabalakrishnan M. Extracellular synthesis of silver nanoparticles using leaf extract of Cassia auriculata. Dig J Nanomater Biostruct 2011; 6(1): 279–83.
20. Jadou A, Al-Shahwany AW. Biogenic synthesis and characterization of silver nanoparticles using some medical plants and evaluation of their anti-bacterial and toxicity potential. J AOAC Int 2018.
21. Salehi S, Shandiz SA, Ghanbar F, Darvish MR, Shafiee Ardestani M, Mirzaie A, et al. Phytos‌yn-thesis of silver nanoparticles using Art-emisia marschalliana Sprengel aerial partextract and assessment oftheir antioxidant, anticancerand antibacterial properties. Int J Nanomedicine 2016; 11: 1835–46.
22. Rasheed T, Bilal M, Iqbal HMN, Li C. Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential bio‌medical applications. Colloids Surf B Bioi‌nterf-aces 2017; 158: 408-15.
23. Khalili H, Baghbani-arani F. Green Synthesized of Silver Nanoparticles Using Artemisia tsche‌rnie‌viana Extract and Evaluation of Cytot-oxi‌city Effects on Human Colon Cancer (HT29) and Normal (HEK293) Cell Lines. Sjimu 2017; 25(2): 91-100. [in Persian]
24. Bora KS, Sharma A. The genus Artemisia: a comprehensive review. Pharm Biol 2011; 49(1): 101-9.
25. Abad MJ, Bedoya LM, Apaza L, Bermejo P. The Artemisia L. Genus: a review of bioactive essential oils. Molecules 2012; 17(3): 2542-66.
26. Redfern J, Kinninmonth M, Burdass D, Verran J. Using soxhlet ethanol extraction to produce and test plant material (essential oils) for their anti-microbial properties. J Microbiol Biol Educ 2014; 15(1): 45-6.
27. Mousavi B, Tafvizi F, Zaker Bostanabad S. Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif Cells Nanomed Biotechnol 2018; 23: 1-12.
28. Wypij M, Czarnecka J, Świecimska M, Dahm H, Rai M, Golinska P. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World J Microbiol Biotechnol 2018; 34(2): 23.
29. Satyavani K, Gurudeeban S, Ramanathan T, Balasubramanian T. Toxicity Study of Silver Nanoparticles Synthesized from Suaeda monoica on Hep-2 Cell Line. Avicenna J Med Biotechnol 2012; 4(1): 35-9.
30. Lalitha P. Apoptotic efficacy of biogenic silver nanoparticles on human breast cancer MCF-7 cell lines. Prog Biomater 2015; 4(2-4): 113-21.
31. Rónavári A, Kovács D, Igaz N, Vágvölgyi C, Boros IM, et al. Biological activity of green-synthesized silver nanoparticles depends on the applied natural extracts: a comprehensive study. Int J Nanomedicine 2017; 12: 871-83.
32. Tang HQ, Hu J, Yang L, Tan RX. Terpenoids and flavonoids from Artemisia species. Planta Med 2000; 66(4): 391-3.
33. Gliga AR, Skoglund S, Wallinder IO, Fadeel B, Karlsson HL. Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release. Part Fibre Toxicol 2014; 11: 11.
34. Sulaiman GM, Mohammed WH, Marzoog TR, Al-Amiery AA, Kadhum AA, Mohamad AB. Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. Asian Pac J Trop Biomed 2013; 3(1): 58-63.
35. Patra JK, Baek KH. Biosynthesis of silver nanoparticles using aqueous extract of silky hairs of corn and investigation of its antibacterial and anti-candidal synergistic activity and antioxidant potential. IET Nanobiotechnol 2016; 10(5): 326-33.
36. Vijayakumar M, Priya K, Nancy FT, Noorlidah A, Ahmed AB. Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica. Ind Crops Prod 2013; 41: 235-40.
37. Xia QH, Zheng LP, Zhao PF, Wang JW. Biosynthesis of silver nanoparticles using Artemisia annua callus for inhibiting stem-end bacteria in cut carnation flowers. IET Nanobiotechnol 2017; 11(2): 185-92.
38. Inbathamizh L, Mekalai P, Jancy E. In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles. J Pharm Res 2013; 6(1): 32-8.
39. Turner A, Brice D, Brown MT. Interactions of silver nanoparticles with the marine macroalga, Ulva lactuca. Ecotoxicology 2012; 21(1): 148-54.
40. Ghavami S, Hashemi M, Ande SR, Yeganeh B, Xiao W, et al. Apoptosis and cancer: mutations within caspase genes. J Med Genet 2009; 46(8): 497-510.
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Moulaie S, Mirzaie A, Aliasgari E. Antibacterial and anticancer activities of silver nanoparticles fabricated by the Artemisia scoparia extract against lung cancer cell line (A549). Feyz Med Sci J 2018; 22 (5) :487-496
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Volume 22, Issue 5 (Bimonthly 2018) Back to browse issues page
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