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Biological synthesis of silver nanoparticles using Artemisia scoparia extract and evaluation of their antibacterial, anticancer activities and analysis of apoptosis gene expression using Real Time PCR
Amir Mirzaie * , Somaye Moulaie , Elahe Aliasgari
Islamic Azad University , Amir_mirzaie92@yahoo.com
Abstract:   (49 Views)
Background: In recent years, due to an increase in the incidence of cancer deaths, there is a need to find new ways to control cancer. The aim of this study was synthesis of silver nanoparticle using Artemisia scoparia extract, cytotoxicity and analysis of apoptosis gene (Bax and Bcl2) expression. 
Materials and Methods: In this experimental study, silver nanoparticles was synthesized using A. scoparia extract. For confirmation of silver nanoparticles synthesis, scanning electron microscope (SEM) and transmission electron microscope (TEM) was used. Subsequently, antibacterial activity of synthesized silver nanoparticles was evaluated. In addition to, cell toxicity of silver nanoparticles against lung cancer cell line in 3.125-100 µg/mL concentration was performed using MTT method. Finally, the gene expression of Bax and Bcl2 was measured using Real Time PCR.  
Results: The results of SEM and TEM show that the synthesized silver nanoparticles had 33.40 average size. The MIC results demonstrated that the silver nanoparticle had antibacterial activity. In addition to, the MTT results indicated that silver nanoparticles had dose-dependent cell toxicity. The Real Time PCR results revealed that the Bax and Bcl2 gene expression was up-regulated and down-regulated, respectively (2.34±0.34, 0.43±0.61). 
Conclusion: Based on the results of this study, the synthesized silver nanoparticle had antibacterial and anticancer activities. 
Keywords: Silver nanoparticle, Artemisia scoparia, MTT, Real Time PCR
     
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/02/20 | Accepted: 2018/08/8
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