[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 23, Issue 4 (Bimonthly 2019) ::
Feyz 2019, 23(4): 334-343 Back to browse issues page
Evaluation of synergistic antimicrobial activity of silver nanoparticles and Eucalyptus microtheca on streptococcus mutans
Ramin Farrokhpour gharehgheshlaghi , Younes Anzabi * , Hoda Jafarizadeh-Malmiri
Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran. , anzabi_y_576@yahoo.com
Abstract:   (1102 Views)
Background: Streptococcus mutans is a main bacteria caused by tooth decay. In the present study, antibacterial activities of the essential oil and hydroalcoholic extract of EucalyptusMicrotheca and silver nanoparticles (Ag­NPs) as compared to some different standard antibiotics, was evaluated against S. mutans.
Materials and Methods: In this experimental study, bactericidal activities of the mentioned essential oil, extract, Ag­NPs, and the selected antibiotics including Amoxicillin-Clavulanic acid, Chloramphenicol, and Ciprofloxacin were evaluated using the agar disc diffusion method against S. mutans. The main bioactive compounds presented in the essential oil and extract of E.­microtheca were detected using gas chromatography technique (GC-MS). All the obtained results were statistically analyzed using SPSS software and the comparison test using Dunken with 95% of significance level was used.
Results: The results obtained indicated that maximum clear zone area with diameter of 23±0.2 mm was observed for the samples containing essential oil and extract of E.­microtheca  and Ag­NPs. While the minimum clear zone (7±0.1 mm) war related to the sample containing essential oil of E.­microtheca and Ag­NPs. The results also indicated that the clear zone diameter for the samples containing Amoxicillin-Clavulanic acid, Chloramphenicol, and Ciprofloxacin were 8±0.1, 22±0.2 and 9±0.1 mm, respectively. GC-MS analysis indicated that there were 44 bioactive compounds in the essential oil of E.­microtheca which Eucalyptol, alpha-Pinene and L-trans-­Pinocarol were the most important components. Furthermore, the extract of E.­microtheca was contained 30 bioavtive compounds which Eucalyptol, Globulol and Aroman dendron were its main components.
Conclusion: The results showed that silver nanoparticles in combination with extract and essential oil of E. microtheca have antimicrobial activity against S.­mutans.
Keywords: Anti-streptococcus properties, Eucalyptus, Essential oil, Extract, Silver nanoparticles
Full-Text [PDF 385 kb]   (380 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/12/9 | Accepted: 2019/06/1 | Published: 2019/09/30
1. Ghapanchi J, Moattari A, Lavaee F, Shakib M. The antibacterial effect of four mouth washes against Streptococcus mutans and Escherichia coli. J Pak Med Assoc 2015; 65(7): 350-3.
2. Facklam R. What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 2002; 15(4): 613-30.
3. Davey AL, Rogers AH. Multiple types of the bacterium Streptococcus mutans in the human mouth and their intra-family transmission. Arch Oral Biol 1984; 29(6): 453-60.
4. Siddiqui B, Sultana Im, Begum S. Triterpenoidal constituents from Eucalyptus camaldulensis var. Obtusa leaves. Phytochem 2000; 54(8): 861-5.
5. Wang Y, Charkowski AO, Zeng C, Zhu T, Wang H, Chen H. Phylogenetic and Morphological Identification of the Novel Pathogen of Rheum palmatum Leaf Spot in Gansu, China. Mycobiol 2016 1; 44(2): 93-8.
6. Maciel MV, Morais SM, Bevilaqua CM, Silva RA, Barros RS, Sousa RN, et al. Chemical composition of Eucalyptus spp. essential oils and their insecticidal effects on Lutzomyia longipalpis. Vet Parasitol 2010; 167(1): 1-7.
7. Djenane D, Yangüela J, Amrouche T, Boubrit S, Boussad N, Roncalés P. Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli O157: H7 and Staphylococcus aureus in minced beef. Food Sci Tech Int 2011; 17(6): 505-15
8. Falahati M, Omidi Tabrizib N, Jahaniani F. Anti dermatophyte activities of Eucalyptus camaldulensis in comparison with Griseofulvin. Iran J Pharm Thera 2005; 4(2): 80.
9. Iqbal Z, Hussain I, Hussain A, Ashraf MY. Genetic variability to essential oil contents and composition in five species of Eucalyptus. PAK J BOT 2003; 35(5): 843-52.
10. Sharifi-Rad J, Sureda A, Tenore G, Daglia M, Sharifi-Rad M, Valussi M, Tundis R, Sharifi-Rad M, Loizzo M, Ademiluyi A, Sharifi-Rad R. Biological activities of essential oils: from plant chemoecology to traditional healing systems. Molecules 2017; 22(1): 70.
11. Gilles M, Zhao J, An M, Agboola S. Chemical composition and antimicrobial properties of essential oils of three Australian Eucalyptus species. Food Chem 2010; 119(2): 731-7.
12. Tajkarimi MM, Ibrahim SA‌, Cliver DO. Antimicrobial herb and spice compounds in food. J Food Qual Hazards Control 2010; 21(9): 199-218.
13. Whitesides GM. The 'right' size in nanobiotechnology. Nat Biotechnol 2003; 21(10): 1161.
14. Devi LS, Joshi SR. Antimicrobial and synergistic effects of silver nanoparticles synthesized using soil fungi of high altitudes of eastern Himalaya. Mycobiol 2012; 40(1): 27-34.
15. Anzabi Y, Aghdam VB, Makoui MH, Anvarian M, Mousavinia MN. Evaluation of Antibacterial Properties of Edible Oils and Extracts of A Native Plant, Ziziphora Clinopodioides (Mountains’ Kakoty), on Bacteria Isolated From Urinary Tract Infections. Life Sci J 2013; 10 Suppl 4: 121-7.
16. Manandhar B, Paudel KR, Sharma B, Karki R. Phytochemical profile and pharmacological activity of Aegle marmelos Linn. JIM 2017; 22(1):70.
17. Finegold SM, Martin WJ. Diagnostic microbiology. 6th ed. Missouri: The C.V. Mosby; 1982. p. 532-59.
18. Tabatabaei A, Firuzi R. Animal diseases due to bacteria. 1th ed. Tehran University Press; 2009. P. 206-61.
19. Quinn PJ, Carter ME, Markey BK, Carter GR. Lynton House, London WC1H9LB, England: Mosby; 1994. p. 209-36.
20. Anzabi Y, Javadi A. Evaluation of antibacterial effects of Onions, methanol extracts and some antibiotics against the number of food born bacteria. J Food Microbiol 2017; 3(4): 83-94. [in Persian]
21. Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogen. J Ethnopharmacol 2001; 74(2):‌ 113-23.
22. Nascimento GG, Locatelli J, Freitas PC and Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 2000; 31(4): 247-56.
23. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility test. Approved standard, M100. 28th ed. 2018. CLSI, Wayne, PA 19087 USA. Available at: https://clsi.org/
24. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ. The bactericidal effect of silver nanoparticles. Nanotechno 2005; 16(10): 23-46.
25. Amin M, Abbasi ME, Javadi M, Shahin F, Teymuri RM. Antimicrobial Evaluation of Methanolic Essential Oil of Eucalyptus glubulos leaves against a Number of Oral Cavity Bacteria. Jentashapir J Health Res 2013; 4 Suppl 1: 41-7.
26. Srinivasan D, Nathan S, Suresh T, Perumalsamy PL. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 2001; 74(3): 217-20.
27. Cheng SS, Huang CG, Chen YJ, Yu JJ, Chen WJ, Chang ST. Chemical compositions and larvicidal activities of leaf essential oils from two eucalyptus species. Bioresour Technol 2009; 100(1): 452-6.
28. Chaleshtori RS, Arani NM, Taghizadeh M, Chaleshtori FS, Barfrosh F. Detection of antibiotic resistance pattern in Staphylococcus aureus isolated from ready to eat foods in Kashan. Int J Food Microbiol 2015; 74(2): 101-2.
29. Soleymani N, Sattari M, Sepehriseresht S, Daneshmandi S. Evaluation of reciprocal pharmaceutical effects and antibacterial activity of Bunium persicum essential oil against some Gram positive and Gram negative bacteria. IJM 2010; 4(1): 26-34.
30. Anzabi Y‌, Khaki A. Antibacterial Effects of the Essential Oils and Ethanol Extracts of the Native Plants; Ziziphora Clinopodioides on 3 Species of Urinary Tract Isolated Bacteria in Rats’ Experimental Model. Med J Tabriz Univ Med Sci 2015; 37(3): 18-25. [in Persian]
31. Mandal S, Dwivedi PD, Singh A, Naqvi AA, Bagchi GD. Capillary gas chromatographic analysis of Eucalyptus globulus from different geoclimatic zones in India. J Essent Oil Res 2001; 13(3):196-7.
32. Delaquis PJ, Stanich K, Girard B, Mazza G. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 2002; 74(2): 90-101.
33. Rizzello L, Pompa PP. Nanosilver-based antibacterial drugs and devices: mechanisms, methodological drawbacks, and guidelines. Chem Soc Rev 2014; 43(5): 1501
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Farrokhpour gharehgheshlaghi R, Anzabi Y, Jafarizadeh-Malmiri H. Evaluation of synergistic antimicrobial activity of silver nanoparticles and Eucalyptus microtheca on streptococcus mutans. Feyz. 2019; 23 (4) :334-343
URL: http://feyz.kaums.ac.ir/article-1-3759-en.html

Volume 23, Issue 4 (Bimonthly 2019) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 29 queries by YEKTAWEB 4218