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:: Volume 23, Issue 4 (Bimonthly 2019) ::
Feyz 2019, 23(4): 334-343 Back to browse issues page
Evaluation of synergistic antimicrobial activity of silver nanoparticles and Eucalyptus microtheca on streptococcus mutans
Ramin Farrokhpour gharehgheshlaghi , Younes Anzabi * , Hoda Jafarizadeh-Malmiri
Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran. , anzabi_y_576@yahoo.com
Abstract:   (298 Views)
Background: Streptococcus mutans is a main bacteria caused by tooth decay. In the present study, antibacterial activities of the essential oil and hydroalcoholic extract of EucalyptusMicrotheca and silver nanoparticles (Ag­NPs) as compared to some different standard antibiotics, was evaluated against S. mutans.
Materials and Methods: In this experimental study, bactericidal activities of the mentioned essential oil, extract, Ag­NPs, and the selected antibiotics including Amoxicillin-Clavulanic acid, Chloramphenicol, and Ciprofloxacin were evaluated using the agar disc diffusion method against S. mutans. The main bioactive compounds presented in the essential oil and extract of E.­microtheca were detected using gas chromatography technique (GC-MS). All the obtained results were statistically analyzed using SPSS software and the comparison test using Dunken with 95% of significance level was used.
Results: The results obtained indicated that maximum clear zone area with diameter of 23±0.2 mm was observed for the samples containing essential oil and extract of E.­microtheca  and Ag­NPs. While the minimum clear zone (7±0.1 mm) war related to the sample containing essential oil of E.­microtheca and Ag­NPs. The results also indicated that the clear zone diameter for the samples containing Amoxicillin-Clavulanic acid, Chloramphenicol, and Ciprofloxacin were 8±0.1, 22±0.2 and 9±0.1 mm, respectively. GC-MS analysis indicated that there were 44 bioactive compounds in the essential oil of E.­microtheca which Eucalyptol, alpha-Pinene and L-trans-­Pinocarol were the most important components. Furthermore, the extract of E.­microtheca was contained 30 bioavtive compounds which Eucalyptol, Globulol and Aroman dendron were its main components.
Conclusion: The results showed that silver nanoparticles in combination with extract and essential oil of E. microtheca have antimicrobial activity against S.­mutans.
Keywords: Anti-streptococcus properties, Eucalyptus, Essential oil, Extract, Silver nanoparticles
Full-Text [PDF 385 kb]   (52 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2018/12/9 | Accepted: 2019/06/1 | Published: 2019/09/30
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Farrokhpour gharehgheshlaghi R, Anzabi Y, Jafarizadeh-Malmiri H. Evaluation of synergistic antimicrobial activity of silver nanoparticles and Eucalyptus microtheca on streptococcus mutans. Feyz. 2019; 23 (4) :334-343
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Volume 23, Issue 4 (Bimonthly 2019) Back to browse issues page
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