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:: Volume 22, Issue 1 (Bimonthly 2018) ::
Feyz 2018, 22(1): 83-93 Back to browse issues page
The study of neurotrophic factor genes expression of human adipose stem cells cultured in serum-containing and serum-free media
Arezoo Amiri , Maryam HajiGhasem Kashani * , Mohammad Taghi Ghorbanian
Assistant Professor Faculty of Biology and Institute of Biological Sciences, Damghan University, Damghan, I. R. Iran. , kashani@du.ac.ir
Abstract:   (1469 Views)
Background: Fetal bovine serum (FBS) is immunogenic for human and may transmit infection in the case of transplantation. So, this study aimed to compare the proliferation and survival rates of human adipose stem cells (hASCs), and their neurotropic factor genes expression in serum-containing and serum-free media.
Materials and Methods: In this experimental study, stem cells were extracted from the abdominal subcutaneous adipose tissue of 15 cesarean women and cultured in α-MEM containing 10% of FBS or serum-free medium. The stemness of fourth passage of the cells was confirmed using the flow cytometry method, and their differentiation into adipocytes and osteocytes was also confirmed. Cell proliferation and survival were assessed using hemocytometry and MTT [3- (4,5-Dimethyltiazol-2-yl) -2,5-Diphenyltetrazolium bromide] methods, respectively. In addition, the expression of neurotrophic factor genes was analyzed by the real-time polymerase chain reaction method.
Results: The cells had positive response to CD44, CD73, CD90, and CD105 markers, while they responded negatively to CD34 and CD45 markers and had the ability to differentiate into adipocytes and osteocytes. The survival and proliferation of the cells cultured in the serum-based medium for 48 hours were significantly increased compared to those cultured in the serum-free medium. Moreover, serum resulted in a significant increase in BDNF and NT-3 genes expression, compared to the cells cultured in the serum-free medium.
Conclusions: More suitable cells can be provided for transplantation with serum deletion and culture medium optimization. The results can be matched to find an appropriate replacement for FBS.
Keywords: Human adipose stem cells, Neurotrophic factors, Proliferation, Survival, Serum
Full-Text [PDF 591 kb]   (153 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2017/05/22 | Accepted: 2017/12/3 | Published: 2018/03/10
1. Martin RJ, Hausman GJ, Hausman DB. Regulation of adipose cell development in utero. Proc Soc Exp Biol Med 1998; 219(3): 200-10.
2. Vindigni V, Giatsidis G, Reho F, Dalla Venezia E, Mammana M, Franco B. Adipose derived stem cells: current state of the art and prospective role in regenerative medicine and tissue engineering. Regenerative Med Tissue Eng 2013; 953-78.
3. Kitada M, Dezawa M. Parkinson's disease and mesenchymal stem cells: potential for cell-based therapy. Parkinson's Dis 2012; 2012: 873706.
4. Casteilla L, Planat-Benard V, Laharrague P, Cousin B. Adipose-derived stromal cells: Their identity and uses in clinical trials, an update. World J Stem Cells 2011; 3(4): 25-33.
5. Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res 2007; 100(9): 1249-60.
6. Guo X, Li S, Ji Q, Lian R, Chen J. Enhanced viability and neural differential potential in poor post-thaw hADSCs by agarose multi-well dishes and spheroid culture. Human Cell 2015; 28(4): 175-89.
7. Egashira Y, Sugitani S, Suzuki Y, Mishiro K, Tsuruma K, Shimazawa M, et al. The conditioned medium of murine and human adipose-derived stem cells exerts neuroprotective effects against experimental stroke model. Brain Res 2012; 1461: 87-95.
8. Mitchell JB, McIntosh K, Zvonic S, Garrett S, Floyd ZE, Kloster A, et al. Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers. Stem Cells 2006; 24(2): 376-85.
9. Kern S, Eichler H, Stoeve J, Kluter H, Bieback K. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006; 24(5): 1294-301.
10. Wang X, Zhao Z, Gong J, Zhou S, Peng H, Shatara A, et al. Adipose stem cells-conditioned medium blocks 6-hydroxydopamine-induced neurotoxicity via the IGF-1/PI3K/AKT pathway. Neurosci Lett 2014; 581: 98-102.
11. Razavi S, Razavi MR, Zarkesh Esfahani H, Kazemi M, Mostafavi FS. Comparing brain-derived neurotrophic factor and ciliary neurotrophic factor secretion of induced neurotrophic factor secreting cells from human adipose and bone marrow-derived stem cells. Dev Growth Differ 2013; 55(6): 648-55.
12. Kingham PJ, Kolar MK, Novikova LN, Novikov LN, Wiberg M. Stimulating the neurotrophic and angiogenic properties of human adipose-derived stem cells enhances nerve repair. Stem Cells Dev 2013; 23(7): 741-54.
13. Jones J, Estirado A, Redondo C, Bueno C, Martínez S. Human Adipose Stem Cell–Conditioned Medium Increases Survival of Friedreich's Ataxia Cells Submitted to Oxidative Stress. Stem Cells Dev 2012; 21(15): 2817-26.
14. Chen HT, Lee MJ, Chen CH, Chuang SC, Chang LF, Ho ML, et al. Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures. J Cell Mol Med 2012; 16(3): 582-93.
15. Ogura F, Wakao S, Kuroda Y, Tsuchiyama K, Bagheri M, Heneidi S, et al. Human adipose tissue possesses a unique population of pluripotent stem cells with nontumorigenic and low telomerase activities: potential implications in regenerative medicine. Stem Cells Dev 2014; 23(7): 717-28.
16. Oliva-Olivera W, Coin-Araguez L, Salas J, Lhamyani S, Gentile AM, Sarria Garcia E, et al. Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells. PloS One 2015; 10(12): e0144401.
17. Witkowska-Zimny M, Walenko K. Stem cells from adipose tissue. Cell Mol Biol Lett 2011; 16(2): 236-57.
18. Liu XL, Zhang W, Tang SJ. Intracranial transplantation of human adipose-derived stem cells promotes the expression of neurotrophic factors and nerve repair in rats of cerebral ischemia-reperfusion injury. Int J Clin Exp Pathol 2014; 7(1): 174-83.
19. Zhu Y, Liu T, Song K, Fan X, Ma X, Cui Z. Adipose-derived stem cell: a better stem cell than BMSC. Cell Biochemistry Function 2008; 26(6): 664-75.
20. Yoshimura K, Shigeura T, Matsumoto D, Sato T, Takaki Y, Aiba‐Kojima E, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol 2006; 208(1): 64-76.
21. Aust L, Devlin B, Foster S, Halvorsen Y, Hicok K, Du Laney T, et al. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy 2004; 6(1): 7-14.
22. Nakagami H, Maeda K, Morishita R, Iguchi S, Nishikawa T, Takami Y, et al. Novel autologous cell therapy in ischemic limb disease through growth factor secretion by cultured adipose tissue–derived stromal cells. Arterioscler Thromb Vasc Biol 2005; 25(12): 2542-7.
23. Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM. Surface protein characterization of human adipose tissue‐derived stromal cells. J Cell Physiol 2001; 189(1): 54-63.
24. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002; 13(12): 4279-95.
25. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999; 284(5411): 143-7.
26. Izadpanah R, Trygg C, Patel B, Kriedt C, Dufour J, Gimble JM, et al. Biologic properties of mesenchymal stem cells derived from bone marrow and adipose tissue. J Cell Biochem 2006, 99(5): 1285-97.
27. Ribeiro TB, Duarte AS, Longhini AL, Pradella F, Farias AS, Luzo AC, et al. Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion. Sci Rep 2015; 5: 16167.
28. Wystrychowski W, Patlolla B, Zhuge Y, Neofytou E, Robbins RC, Beygui RE. Multipotency and cardiomyogenic potential of human adipose-derived stem cells from epicardium, pericardium, and omentum. Stem Cell Res Ther 2016; 7(1): 84.
29. Maredziak M, Marycz K, Tomaszewski KA, Kornicka K, Henry BM. The Influence of Aging on the Regenerative Potential of Human Adipose Derived Mesenchymal Stem Cells. Stem Cells Int 2016; 2016: 2152435.
30. Huang S, Wang S, Bian C, Yang Z, Zhou H, Zeng Y, et al. Upregulation of miR-22 promotes osteogenic differentiation and inhibits adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells by repressing HDAC6 protein expression. Stem Cells Dev 2012; 21(13): 2531-40.
31. Airaksinen MS, Saarma M. The GDNF family: signalling, biological functions and therapeutic value. Nat Rev Neurosci 2002; 3(5): 383-94.
32. Conner JM, Franks KM, Titterness AK, Russell K, Merrill DA, Christie BR, et al. NGF is essential for hippocampal plasticity and learning. J Neurosci 2009; 29(35): 10883-9.
33. Chauhan NB, Siegel GJ, Lee JM. Depletion of glial cell line-derived neurotrophic factor in substantia nigra neurons of Parkinson's disease brain. J Chem Neuroanat 2001; 21(4): 277-88.
34. Thoenen H, Sendtner M. Neurotrophins: from enthusiastic expectations through sobering experiences to rational therapeutic approaches. Nat Neurosci 2002; 5(11s): 1046.
35. Deinhardt K, Chao MV. Trk receptors. Handb Exp Pharmacol 2014; 220: 103-19.
36. Mogi M, Togari A, Kondo T, Mizuno Y, Komure O, Kuno S, et al. Brain-derived growth factor and nerve growth factor concentrations are decreased in the substantia nigra in Parkinson's disease. Neurosci Lett 1999; 270(1): 45-8.
37. Gyárfás T, Knuuttila J, Lindholm P, Rantamäki T, Castrén E. Regulation of brain-derived neurotrophic factor (BDNF) and cerebral dopamine neurotrophic factor (CDNF) by anti-parkinsonian drug therapy in vivo. Cell Mol Neurobiol 2010; 30(3): 361-8.
38. Moller JC, Sautter J, Kupsch A. Potential of neurotrophic factors in therapy of Parkinson's disease. J Neural Transm Suppl 1996; 48: 103-12.
39. Tokugawa K, Yamamoto K, Nishiguchi M, Sekine T, Sakai M, Ueki T, et al. XIB4035, a novel nonpeptidyl small molecule agonist for GFRalpha-1. Neurochem Int 2003; 42(1): 81-6.
40. Visanji NP, Orsi A, Johnston TH, Howson PA, Dixon K, Callizot N, et al. PYM50028, a novel, orally active, nonpeptide neurotrophic factor inducer, prevents and reverses neuronal damage induced by MPP+ in mesencephalic neurons and by MPTP in a mouse model of Parkinson's disease. FASEB J 2008; 22(7): 2488-97.
41. Kramer R, Zhang Y, Gehrmann J, Gold R, Thoenen H, Wekerle H. Gene transfer through the blood-nerve barrier: NGF-engineered neuritogenic T lymphocytes attenuate experimental autoimmune neuritis. Nat Med 1995; 1(11): 1162-6.
42. Ghasemi N, Razavi S. Transdifferentiation potential of adipose-derived stem cells into neural lineage and their application. J Histol Histopathol 2014; 1(1): 12.
43. mesenchymal stem cells improves motor function and extends survival in R6/2 transgenic mouse model for Huntington’s disease. PLOS Curr 2012.
44. Lopatina T, Kalinina N, Karagyaur M, Stambolsky D, Rubina K, Revischin A, et al. Adipose-derived stem cells stimulate regeneration of peripheral nerves: BDNF secreted by these cells promotes nerve healing and axon growth de novo. PloS One 2011; 6(3): e17899.
45. Zemel'ko VI, Kozhukharova IB, Alekseenko LL, Domnina AP, Reshetnikova GF, Puzanov MV, et al. Neurogenic potential of human mesenchymal stem cells isolated from bone marrow, adipose tissue and endometrium: a comparative study. Tsitologiia 2013; 55(2): 101-10.
46. Zhong Z, Gu H, Peng J, Wang W, Johnstone BH, March KL, et al. GDNF secreted from adipose-derived stem cells stimulates VEGF-independent angiogenesis. Oncotarget 2016; 7(24): 36829-41.
47. Kim IG, Piao S, Lee JY, Hong SH, Hwang T-K, Kim SW, et al. Effect of an adipose-derived stem cell and nerve growth factor-incorporated hydrogel on recovery of erectile function in a rat model of cavernous nerve injury. Tissue Eng Part A 2012; 19(1-2): 14-23.
48. Fontanilla CV, Gu H, Liu Q, Zhu TZ, Zhou C, Johnstone BH, et al. Corrigendum: Adipose-derived Stem Cell Conditioned Media Extends Survival time of a mouse model of Amyotrophic Lateral Sclerosis. Sci Reports 2016; 6: 20747.
49. Bhang SH, Lee S, Shin JY, Lee TJ, Jang HK. Efficacious and clinically relevant conditioned medium of human adipose-derived stem cells for therapeutic angiogenesis. Mol Ther 2014; 22(4): 862-72.
50. Chase LG, Lakshmipathy U, Solchaga LA, Rao MS, Vemuri MC. A novel serum-free medium for the expansion of human mesenchymal stem cells. Stem Cell Res Ther 2010; 1(1): 8.
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Amiri A, HajiGhasem Kashani M, Ghorbanian M T. The study of neurotrophic factor genes expression of human adipose stem cells cultured in serum-containing and serum-free media. Feyz. 2018; 22 (1) :83-93
URL: http://feyz.kaums.ac.ir/article-1-3342-en.html

Volume 22, Issue 1 (Bimonthly 2018) Back to browse issues page
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