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The effect of eight weeks of moderate-intensity continuous training (MICT) on the protein expression levels of muscle mass-regulating proteins in the MAPK signaling pathway in skeletal muscle tissue of male Wistar rats with type 2 diabetes
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Malihe Bovand    , Elaheh Heidarnia * , Mehrzad Moghadasi |
| Shiraz branch, Islamic Azad University & Shiraz branch, Islamic Azad University , heidarniae@yahoo.com |
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Abstract: (34 Views) |
Background and Aim: Diabetes as a clinical stress can lead to dysfunction in key cellular pathways in skeletal muscles. On the other hand, aerobic exercise training as a physiological stress can lead to the modulation of these disorders. Therefore, the aim of this study was to investigate the effect of eight weeks of moderate-intensity continuous training (MICT) on the protein expression levels of muscle mass-regulating proteins in the MAPK signaling pathway in skeletal muscle tissue of male Wistar rats with type 2 diabetes.
Methods: In this experimental study, 18 rats were selected and purchased. 12 of the rats were induced to type 2 diabetes with a combined protocol of high-fat diet (containing 60% of calories from fat), streptozotocin injection (60 mg/kg body weight) and nicotinamide (110 mg/kg body weight); Then they were randomly divided into 2 groups: MICT diabetic group and control diabetic group (6 rats in each group). A healthy control group (6 heads) was also included. The training protocol was implemented for eight weeks and five sessions per week in a gradual and increasing manner. 48 hours after the last training session, calf muscle tissue samples were removed and protein content was measured by Western blotting. Data analysis was performed using SPSS and GraphPad Prism software. The significance level was considered to be p<0.05.
Results: The protein expression levels of RAF-1 (F (2,15)=183.06; p=0.001; η²=0.961) and p-ERK1 (F (2,15)=181.85; p=0.001; η²=0.960) was significantly increased in the MICT group compared to both the diabetic and healthy control groups. In contrast, a significant decrease in blood glucose levels was observed following the MICT protocol (F=(2,15)=221.64; p=0.001; η²=0.967).
Conclusion: It seems that the increase in RAF-1 and p-ERK1 protein content following eight weeks of MICT may indicate the activation of signaling pathways related to protein synthesis; Therefore, MICT can be considered as an effective non-pharmacological intervention to improve metabolic status and promote muscle health in subjects with type 2 diabetes. |
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| Keywords: Moderate-intensity continuous exercise, type 2 diabetes, MAPK pathway, skeletal muscle |
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Type of Study: Research |
Subject:
General
Received: 2025/10/25 | Revised: 2025/12/1 | Accepted: 2025/12/1
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