Background and Objective: Type 2 diabetes, as a prevalent disease in developing countries, is rapidly increasing. An active lifestyle can play a significant role in managing this condition. Therefore, the present study aimed to investigate the effect of 12 weeks of Pilates training on serum forin levels and some body composition indices in women with type 2 diabetes. Methods: his semi-experimental study included a population of 365 women aged 35–55 years with type 2 diabetes (from the Najafabad Diabetes Association) who had no history of regular physical activity. From the total population, 30 participants were randomly and purposively assigned to experimental and control groups (15 participants per group). The experimental group performed progressive Pilates training for 12 weeks, 3 sessions per week, each lasting 50 minutes, while the control group continued their routine daily activities. Blood samples were taken, and serum forin levels were measured using the ELISA method, while BMI was assessed using the corresponding form, both before and after the intervention. Paired t-tests were used to analyze pre-test and post-test changes Results: In the exercise group, weight (reduction: 2.13 kg, p = 0.04), BMI (reduction: 1.19 kg/m², p = 0.049), fasting blood glucose (reduction: 12.3 mmol/L, p = 0.04), and furin levels (reduction: 6.6 mg/L, p = 0.001) decreased significantly. In the control group, no significant changes were observed in any of the variables (p ≥ 0.05). Conclusion: Pilates training can improve serum furin levels and body composition; therefore, Pilates may serve as a non-pharmacological therapeutic approach for women with type 2 diabetes.