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:: Volume 28, Issue 2 (Bimonthly 2024) ::
Feyz Med Sci J 2024, 28(2): 183-192 Back to browse issues page
The expression level of miR-21, a target of the PTEN gene receptor in the PI3K signaling pathway, in clinical samples of triple negative breast cancer
Javad Razaviyan , Razieh Hadavi , Melika Tafti , Samira Mohammadi-Yeganeh *
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , smyeganeh@gmail.com
Abstract:   (507 Views)
Background and Aim: Triple negative breast cancer (TNBC) is an aggressive form of breast cancer, necessitating effective treatment methods. This study aimed to assess the expression level of miR-21, a target of the Phosphatase and tensin homolog (PTEN) gene receptor in the PI3K signaling pathway, in clinical samples of TNBC.
Methods: Fifteen TNBC tumor samples and 15 adjacent normal tissue samples were obtained from the tumor bank of Imam Khomeini Hospital in Tehran, Iran. RNA extraction, cDNA synthesis, and Quantitative Real-Time PCR (qRT-PCR) analysis were conducted. Relative expression of PTEN and miR-21 was analyzed using REST 2009® software, with gene expression also evaluated using the UALCAN database. Additionally, ROC curve analysis was performed to assess the diagnostic value of miR-21 in TNBC.
Results: qRT-PCR analysis revealed a significant decrease in PTEN expression in most clinical samples (13 samples) by 4.6-fold, and a decrease in miR-21 target expression by 6.8-fold in 10 samples and 8.9-fold in 5 samples compared to normal tissue. These findings were supported by results from the UALCAN database. Furthermore, ROC curve analysis demonstrated the diagnostic potential of miR-21 in TNBC, with an area under the curve of 0.742.
Conclusion: The study suggests that miR-21 could serve as a promising candidate for inhibiting PTEN gene expression, potentially aiding in targeted treatment for TNBC. However, further research in this area is recommended.
Keywords: Triple-negative breast cancer, miR-21, PTEN
Full-Text [PDF 613 kb]   (379 Downloads)    
Type of Study: Research | Subject: General
Received: 2024/03/11 | Revised: 2024/08/18 | Accepted: 2024/05/20 | Published: 2024/06/2
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Razaviyan J, Hadavi R, Tafti M, Mohammadi-Yeganeh S. The expression level of miR-21, a target of the PTEN gene receptor in the PI3K signaling pathway, in clinical samples of triple negative breast cancer. Feyz Med Sci J 2024; 28 (2) :183-192
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