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:: Volume 27, Issue 1 (Bimonthly 2023) ::
Feyz Med Sci J 2023, 27(1): 21-30 Back to browse issues page
Preparation of niosomal nanostructure containing Brucella trivalent immunogen as a vaccine candidate
Fahimeh Sharif , Razieh Nazari , Mahdi Fasihi-Ramandi * , Ramezan Ali Taheri , Mohsen Zargar
Molecular Biology Research Center , Baqiyatallah University of Medical Sciences , Tehran , Iran. , fasihi.m@gmail.com
Abstract:   (1281 Views)
Background: Brucellosis is one of the most common diseases between humans and animals. Therefore, the need for prevention and production of an efficient vaccine is necessary. The aim of the present study is to prepare a niosomal nanostructure containing trivalent immunogen (TF, Bp26 and Omp31) of Brucella as a nanovaccine candidate.
Materials and Methods: In this study, after designing the immunogen structure by bioinformatics databases and software, in order to evaluate the immunogenicity of the designed recombinant protein, clone and gene expression were carried out in E.coli BL21. The protein extracted from the culture of said cells was purified by Ni-NTA column. Thin layer coating method was used to prepare niosomes carrying trivalent immunogen and were evaluated using DLS and Zetasizer characterization tests. Then, the amount of trivalent immunogen loading and release was calculated.
Results: The results of the characterization confirmed the successful fabrication of niosomal nanosystem containing trivalent immunogen. The results showed that the produced niosomes are in a suitable range of size (100 nm). Trivalent immunogen with high efficiency (81.96%) is encapsulated in the system. The release of trivalent immunogen from Niosomal nanosystem was reported to be 97% after 96 hours. In addition, the trivalent immunogen release pattern of the coated system was more controlled and slower. This confirms the positive effect of the niosome nanosystem.
Conclusion: The use of nisome as a nanovaccine agent has an effective role in controlling antigen release and can be considered as a vaccine candidate and increase the protective immune response against Brucella.
Keywords: Brucella, vaccine, Nanostructure, TF, Bp26, Omp31
Full-Text [PDF 427 kb]   (866 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2022/12/19 | Revised: 2023/07/25 | Accepted: 2023/01/15 | Published: 2023/04/5
References
1. Avijgan M, Rostamnezhad M, Jahanbani-Ardakani H. Clinical and serological approach to patients with brucellosis: A common diagnostic dilemma and a worldwide perspective. Microb Pathog 2019;129:125-30.
2. Chaudhuri P, Saminathan M, Ali SA, Kaur G, Singh SV, Lalsiamthara J, et al. Immunization with Brucella abortus S19Δper Conferred Protection in Water Buffaloes against Virulent Challenge with B. abortus Strain S544. Vaccines 2021; 9(12): 1423.
3. Jawetz M. Medical Microbiology 27 edition: Lange; 2016.
4. Murray PR, Rosenthal KS, Pfaller MA. Medical microbiology E-book: Elsevier Health Sciences; 2020.
5. Gomez G, Adams LG, Ficht AR, Ficht TA. Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. Frontiers Cell Infect Microb 2013; 3: 17.
6. Kumar DR, Sivalingam J, Mishra SK, Kumar A, Vineeth M, Chaudhuri P, et al. Differential expression of cytokines in PBMC of Bos indicus and Bos taurus× Bos indicus cattle due to Brucella abortus S19 antigen. Animal Biotech 2020; 31(2): 148-54.
7. Atabey T, Acar T, Derman S, Ordu E, Erdemir A, Taşlı PN, et al. In Vitro Evaluation of Immunogenicity of Recombinant OMP25 Protein Obtained from Endemic Brucella abortus Biovar 3 as Vaccine Candidate Molecule Against Animal Brucellosis. Protein Peptide Lett 2021; 28(10): 1138-47.
8. Abkar M, Fasihi-Ramandi M, Kooshki H, Lotfi AS. Oral immunization of mice with Omp31-loaded N-trimethyl chitosan nanoparticles induces high protection against Brucella melitensis infection. Int J Nano. 2017;12:8769.
9. Abdollahi A, Mansouri S, Amani J, Fasihi-Ramandi M, Ranjbar R, Ghasemi A, et al. A Recombinant Chimera Protein as a Novel Brucella Subunit Vaccine: Protective Efficacy and Induced Immune Response in BALB/c Mice. Jundishapur J Microbiol 2018; 11(1): 1-9. [in Persian]
10. Gopalakrishnan A, Dimri U, Saminathan M, Yatoo M, Priya GB, Gopinath D, et al. Virulence factors, intracellular survivability and mechanism of evasion from host immune response by brucella: an overview. J Animal Plant Sci 2016; 26(6).
11. Abkar M, Alamian S, Sattarahmady N. A comparison between adjuvant and delivering functions of calcium phosphate, aluminum hydroxide and chitosan nanoparticles, using a model protein of Brucella melitensis Omp31. Immunol Lett 2019; 207: 28-35.
12. Moghassemi S, Hadjizadeh A. Nano-niosomes as nanoscale drug delivery systems: an illustrated review. J Controlled Release 2014; 185: 22-36.
13. Hasan AA, Madkor H, Wageh S. Formulation and evaluation of metformin hydrochloride-loaded niosomes as controlled release drug delivery system. Drug Delivery 2013; 20(3-4): 120-6.
14. Bagheri-Josheghani S, Bakhshi B. Formulation of selenium nanoparticles encapsulated by alginate-chitosan for controlled delivery of Vibrio Cholerae LPS: A novel delivery system candidate for nanovaccine. Int J Biol Macromol 2022; 208: 494-508.
15. Fasihi-Ramandi M, Ghobadi-Ghadikolaee H, Ahmadi-Renani S, Taheri RA, Ahmadi K. Vibrio cholerae lipopolysaccharide loaded chitosan nanoparticle could save life by induction of specific immunoglobulin isotype. Artif Cells Nanomed Biotechnol 2018; 46(1): 56-61.
16. Sekhavati MH, Heravi RM, Tahmoorespur M, Yousefi S, Abbassi-Daloii T, Akbari R. Cloning, molecular analysis and epitopics prediction of a new chaperone GroEL Brucella melitensis antigen. Iran J Basic Med Sci 2015; 18(5): 499.
17. Díaz AG, Clausse M, Paolicchi FA, Fiorentino MA, Ghersi G, Zylberman V, et al. Immune response and serum bactericidal activity against Brucella ovis elicited using a short immunization schedule with the polymeric antigen BLSOmp31 in rams. Vet Immunol Immunopathol 2013; 154(1-2): 36-41.
18. Abkar M, Lotfi A, Amani J, Ghorashi S, Brujeni G, Kamali M. Design of a chimeric DNA vaccine against Brucella spp. Minerva Biotec 2014; 26(4): 223-33.
19. Ghasemi A, Ranjbar R, Amani J. In silico analysis of chimeric TF, Omp31 and BP26 fragments of Brucella melitensis for development of a multi subunit vaccine candidate. Iran J Basic Med Sci 2014; 17(3): 172.
20. Ahmed IM, Khairani-Bejo S, Hassan L, Bahaman AR, Omar AR. Serological diagnostic potential of recombinant outer membrane proteins (rOMPs) from Brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay. BMC Vet Res 2015; 11(1): 1-0.
21. Pakzad I, Rezaee A, Rasaee MJ, Hossieni AZ, Tabbaraee B, Kazemnejad A. Protection of BALB/C mice against Brucella abortus 544 challenge by vaccination with combination of recombinant human serum albumin-l7/l12 (Brucella abortus ribosomal protein) and lipopolysaccharide. Romanian Arch 2010: 5.
22. Khan S, Akhtar MU, Khan S, Javed F, Khan AA. Nanoniosome‐encapsulated levoflaxicin as an antibacterial agent against Brucella. J Basic Microbiol 2020; 60(3): 281-90.
23. Mukherjee F, Prasad A, Bahekar VS, Rana SK, Rajendra L, Sharma GK, Srinivasan VA. Evaluation of immunogenicity and protective efficacy of a liposome containing Brucella abortus S19 outer membrane protein in BALB/c mice. Iran J Vet Res 2016; 17(1): 1.
24. Amiri B, Ebrahimi-Far M, Saffari Z, Akbarzadeh A, Soleimani E, Chiani M. Preparation, characterization and cytotoxicity of silibinin-containing nanoniosomes in T47D human breast carcinoma cells. Asian Pac J Cancer Prev 2016; 17(8): 3835-8. [in Persian]
25. Taymouri S, Varshosaz J. Effect of different types of surfactants on the physical properties and stability of carvedilol nano-niosomes. Adv Biomed Res 2016; 5.
26. Amoabediny G, Haghiralsadat F, Naderinezhad S, Helder MN, Akhoundi Kharanaghi E, Mohammadnejad Arough J, Zandieh-Doulabi B. Overview of preparation methods of polymeric and lipid-based (niosome, solid lipid, liposome) nanoparticles: A comprehensive review. Int J Polym Mater 2018 ; 67(6): 383-400.
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Sharif F, Nazari R, Fasihi-Ramandi M, Taheri R A, Zargar M. Preparation of niosomal nanostructure containing Brucella trivalent immunogen as a vaccine candidate. Feyz Med Sci J 2023; 27 (1) :21-30
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Volume 27, Issue 1 (Bimonthly 2023) Back to browse issues page
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