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Investigation of the expression of P53 gene in bystander cells after therapeutic ultrasound exposure
Masumeh Rezaei , Kamran Samani Kamran Samani , Ahmad Shanei * , Mohammad Kazemi , Seyed Hossein Hejazi
Department of Medical Physic , a.shanei1397@gmail.com
Abstract:   (56 Views)
Background: Bystander effect is defined as biological responses observed in cells that are not exposed to radiation these radiation effects through messages from irradiated cells. One of the non-invasive method for cancer treatment is ultrasound waves. The therapeutic efficacy of ultrasound waves, like treatment with ionizing radiation, can be altered by the bystander effect, so it is of the bystander effect after ultrasound exposure. In this study, the effect of ultrasound exposure on the expression of P53 gene in neighboring cells has been investigated.
Methods: The cells were divided into three groups: control (without treatment), target (under direct ultrasound exposure) and (no radiation, only receiving target culture media). In the target group, the cells were exposed to ultrasound and after one hour their culture medium to the bystander group. Cell viability and the expression of P53 gene by using the MTT assay and Real-time PCR was performed.
Findings: The percentage of cell survival in the target group was reduced compared to the control group, but in the bystander group, this difference was not significant. The expression of P53 gene in the bystander group was statistically increased when compared with the control group.
Conclusion: The bystander effect is induced after ultrasound exposure and it can increase the level of P53 gene expression in bystander cells.
 
Keywords: Ultrasound waves, Bystander effect, MTT assay, Real-time PCR, P53 gene
     
Type of Study: Research | Subject: medicine, paraclinic
Received: 2019/01/10 | Accepted: 2019/06/1
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