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The effect of chronic administration of caffeine on pentylenetetrazole-induced seizure threshold in mice and nitric oxide metabolites
Zahra Esmaili , Azhdar Heydari *
Associate Professor Kashan University of Medical Sciences , heydariazh@gmail.com
Abstract:   (138 Views)
Background: Long-term caffeine intake decreases seizure susceptibility and has protective effect. This protective effect of caffeine may be somewhat through blockade of A2A adenosine receptors. Considering that activation of adenosine A2A receptors elevates nitric oxide production, the aim of present study was to investigate the effect of chronic caffeine intake on pentylenetetrazole (PTZ)-induced seizure threshold and measurement of nitric oxide metabolites levels in mice.
Materials and Methods: In this study, NMRI male mice (weighing 25-30 g) divided into 3 groups including one control and two experimental groups (n=9 in each group). PTZ- induced clonic seizure was measured following oral intake of caffeine in experimental groups (100 and 300 mg/L in the drinking water) or only tap water in control group for 90 days. Measurement of nitric oxide metabolites in the brain tissues was done at the end of experiments with the Greiss method.
Results: Chronic caffeine intake at concentrations of 100 and 300 mg/L in the drinking water for 90 days did not change seizure threshold. On the other hand, both concentrations of caffeine significantly decreased nitric oxide metabolites levels compared to the control group (p<0.05).
Conclusion: The results of this study confirmed and extended previous studies that chronic caffeine intake has no effect against seizure. Lowered levels of nitric oxide metabolites and resulrtant decreased neuronal excitability may be the main protective mechanism of chronic caffeine intake. Blockade of the A2A adenosine receptors following chronic caffeine intake may be involved in lowered levels of nitric oxide metabolites.
Keywords: caffeine, seizure, pentylenetetrazole, nitric oxide, adenosine receptor
Type of Study: Research | Subject: General
Received: 2018/05/28 | Accepted: 2018/06/24
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