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:: Volume 22, Issue 3 (Bimonthly 2018) ::
Feyz 2018, 22(3): 274-282 Back to browse issues page
Evaluation of the simultaneous effect of zinc oxide nanoparticles and vitamin C on oxidative stress in rat cerebellum
Maryam Rafieirad * , Saeed Valipour-Chahardah-Charic
Assistant Professor Department of Biology, Faculty of Basic Sciences, Izeh Branch, Islamic Azad University, Izeh, I. R. Iran. , rafieirad.m@gmail.com
Abstract:   (286 Views)
Background: In recent years, the potential effects of nanoscale materials on the central nervous system have become even more noticeable. The cerebellum is one of the areas of the brain with high absorption of zinc. The aim of this study was to investigate the effects of zinc oxide nanoparticles (NPs) on oxidative stress indices in rat cerebellum in the presence and absence of vitamin C.
Materials and Methods: In this experimental study, male Wistar rats (250-200g) were divided into the following groups (n=7): a normal saline group (control), three groups that received different doses of zinc oxide NPs (1.25, 2.5 and 5 mg/kg), three groups that received different doses of vitamin ­C (30, 60 and 120 mg/kg) and three groups that received 1.25, 2.5 and 5 mg/kg of zinc oxide NPs combined with 30 mg/kg of vitamin­ C. Half an hour after receiving the medication, the rats were anesthetized and then their cerebellum was removed and the malondialdehyde (MDA) and thiol groups of the cerebellum region were measured.
Results: The MDA level decreased significantly in the groups received 5mg/kg of zinc oxide NPs, in the groups received different doses of vitamin­ C, and in the groups received different doses of zinc oxide NPs combined with 30 mg/kg vitamin­ C compared with the saline group. Total thiol concentration did not change significantly in the different study groups compared to the control group.
Conclusion: Zinc oxide NPs alone or in combination with vitamin C has a reduced effect on oxidative stress in the rat cerebellum.
Keywords: Zinc oxide NPs, Malondialdehyde, Thiol, Vitamin C, Rats
Full-Text [PDF 466 kb]   (44 Downloads)    
Type of Study: Research | Subject: General
Received: 2018/01/31 | Accepted: 2018/05/7 | Published: 2018/08/4
References
1. Dawei A, Zhisheng W, Anguo Z. Protective effects of Nano-ZnO on the primary culture mice intestinal epithelial cells in in vitro against oxidative injury. World J Agricultural Sci 2010; 6(2): 149-53.
2. Nunes A, Al-Jamal KT, Kostarelos K. Thera‌peutics, imaging and toxicity of nanomaterials in the central nervous system. J Control Release 2012; 161(2): 290-306.
3. Kesmati M, Torabi M, Ghandizadeh-Dezfuli M. Nanoparticles of Zinc Oxide Reduces Acute Somatic Pain in Adult Female Wistar Rats. Zahe‌dan J Res Med Sci 2014; 16(2): 24-8. [in Persian]
4. Sharma HS, Sharma A. Nanoparticles aggravate heat stress induced cognitive deficits, blood-brain barrier disruption, edema formation and brain pathology. Prog Brain Res 2007; 162: 245–73.
5. Kreuter J, Shamenkov D, Petrov V. Apolipo‌protein-mediated transport of nanoparticle-bound drugs across the blood-brain barrier. J Drug Tar‌get 2002; 10(4): 317–25.
6. Han D, Tian Y, Zhang T, Ren G, Yang Z. Nano-zinc oxide damages spatial cognition capability via over-enhanced long-term potentiation in hippo‌campus of Wistar rats. Int J Nanomedicine 2011; 6: 1453
7. Karlsson HL, Gustafsson J, Cronholm P, Moller L. Size-dependent toxicity of metal oxide particles--a comparison between nano- and micrometer size. Toxicol Lett 2009; 188(2): 112-8.
8. Sanz A, Fernandez-Ayala DJ, Stefanatos RK, Jacobs HT. Mitochondrial ROS production corr‌elates with, but does not directly regulate lifespan in Drosophila. Aging (Albany NY) 2010; 2 (4): 200
9. Toduka Y, Toyooka T, Ibuki Y. Flow Cytometric Evaluation of Nanoparticles Using Side-Scattered Light and Reactive Oxygen Species-Mediated Fluorescence–Correlation with Geno‌to‌xicity. Environ Sci Technol 2012; 46(14): 7629–36.
10. Sanz A, Stefanatos RK. The mitochondrial free radical theory of aging: a critical view. Curr Aging Sci 2008; 1(1): 10-21.
11. Evans MD, Cooke MS. Factors contributing to the outcome of oxidative damage to nucleic acids. Bioessays 2004; 26(5): 533-42.
12. Traber MG, Atkinson J. Vitamin E, antioxidant and nothing more. Free Radic Biol Med 2007; 43 (1): 4-15.
13. Padayatty SJ, Katz A, Wang Y, Eck P, Kwon O, Lee JH, et al. Vitamin C as an antioxidant: eval‌uation of its role in disease prevention. J Am Coll Nutr 2003; 22 (1): 18-35.
14. kesmati M, Torabi M, Malek Shahi Nia H, Teymuri Zamaneh H. Effect of chronic admin‌istration of zinc supplements (ZnO and nano ZnO) with and without aerobic exercise on nociception in male rats. J Physiol Pharmacol 2013; 16: 415-22.
15. Valipour-Chahardah-Charic S, Kesmati M, Vahdati A, Hoseiny SE. Oxidative stress indices in rat hippocampus using the memory deficit model induced by zinc oxide nanoparticles. Feyz 2015; 19(1): 38-46. [in Persian]
16. peroxidation in animal model of Parkinson disease in adult male rats. Res J Pharmacognosy 2017; 4(2): 53-63.
17. Mansouri MT, Farbood Y, Sameri MJ, Sarkaki A, Naghizadeh B, Rafeirad M. Neuroprotective effects of oral gallic acid against oxidative stress induced by 6-hydroxydopamine in rats. Food Chem 2013; 138 (2-3): 1028-33.
18. Sharma V, Shukla RK, Saxena N, Parmar D, Das M, Dhawan A. DNA damaging potential of zinc oxide nanoparticles in human epidermal cells. Toxicol Lett 2009;185 (3):211-8.
19. Ji J, Long Z, Lin D. Toxicity of oxide nano‌pa‌rt‌icles to the green algae Chlorella sp. Chem Eng J 2011; 170 (2-3): 525-30.
20. Sanjay SS, Pandey AC, Kumar S, Pandey AK. Cell membrane protective efficacy of ZnO nano‌particles. Sop Transactions Nanotechnol 2014; 1: 21-9.
21. Hu ML. Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol 1994; 233: 380-5.
22. Chen X, Zhou Y, Peng X, Yoon J. Fluorescent and colorimetric probes for detection of thiols. Chem Society Reviews 2010; 39(6): 2120-35.
23. Matsunami M, Kirishi S, Okui T, Kawabata A. Chelating luminal zinc mimics hydrogen sulfide-evoked colonic pain in mice: possible involvement of T-type calcium channels. Neuroscience 2011; 181: 257-64.
24. Liu H, Wang H, Sheng M, Jan LY, Jan YN, Basbaum AI. Evidence for Presynaptic N-Methyl-D-Aspartate Autoreceptors in the Spinal Cord Dorsal Horn. PNAS 1994; 91(18): 8383-7.
25. Qiu S, Li L, Weeber EJ, May JM. Ascorbate transport by primary cultured neurons and its role in neuronal function and protection against excito‌toxicity. J Neurosci Res 2007; 85 (5): 1046-56.
26. Rafieirad M, Valipour Chahardah Charic S. Effect of Zinc Oxide Nano-Particles on Motor Coordination in the Attendance of Vitamin C in Rats. Report Health Care 2017; 3(2): 1-6.
27. May J.M. Vitamin C transport and its role in the central nervous system. Subcell Biochem 2012; 56: 85-103.
28. Iwata N, Okazaki M, Xuan M , Kamiuchi S, Hibino Y. Orally Administrated Ascorbic Acid Suppresses Neuronal Damage and Modifies Expression of SVCT2 and GLUT1 in the Brain of Diabetic Rats with Cerebral Ischemia-Reperfusion. Nutrients 2014; 6(4): 1554–77.
29. Brahma B, Forman RE, Stewart EE, Nicholson C, Rice ME. Ascorbate inhibits edema in brain slices. J Neurochem 2000; 74(3):1263-70.
30. Kheiry M, Kesmati M, Najaf Zadeh H, Fatemi SR. Effect of ZnO Nanoparticles on Morphine Dependence in Mice in the Presence and Absence of Vitamin C. AMUJ 2015; 17: 31-40. [in Persian]
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Rafieirad M, Valipour-Chahardah-Charic S. Evaluation of the simultaneous effect of zinc oxide nanoparticles and vitamin C on oxidative stress in rat cerebellum. Feyz. 2018; 22 (3) :274-282
URL: http://feyz.kaums.ac.ir/article-1-3525-en.html


Volume 22, Issue 3 (Bimonthly 2018) Back to browse issues page
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