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Cytotoxic effect of Sargassum glaucescens extract from Chabahar coast instead of the breast (MCF-7) and colorectal (HT-29) cancer cell lines
Ali Taheri * , Mgmostafa Ghaffari , Zeinab Bavi , Fariborz Sohili
, taherienator@gmail.com
Abstract:   (136 Views)
Background: Marine algae are one of the natural resources which produce a wide range of new secondary metabolites with various biological activities same as cytotoxic effects. The present study intended to evaluate the cytotoxic effect of marine algae Sargassum glaucescens extract collected from Chababr coasts instead of the colorectal and breast cancer cell lines.
Materials and method: Study the Cell toxicity of methanol, chloroform, ethyl acetate, and hexan extract of the marine algae in four concentrations of 125, 250, 500 and 1000 μg/mL by MTT and trypan blue assays on two type of cell lines (MCF-7 and HT-29) was investigated. DNA fragmentation activity of the cells was also evaluated by the electrophoresis method. Experimental results of this study were presented as mean ± standard deviation of three replications and for analyzing data by the use of one-way ANOVA.
Results: The survival of cancer cells, decreased with increasing concentration. Concentration of 1000 (µg/ml) of methanol extract showed the greatest effect compared with controls (p<0.05) and the other concentrations. The LC50 amount of methanol extract on colorectal and breast cancer cell lines was (630.8±16.37) and (774.01±28.07 µg/ml), respectively. Also, methanol extract of algae was able to fragment the DNA of cancer cells and induce apoptosis.
Conclusion: In this study the cytotoxic effects of algae extract observed that could be the basis for further studies in order to achieve new pharmaceuticals for treating cancer. Also, after preclinical and clinical studies the extract can be used in food and pharmaceutical industries.
Keywords: Cytotoxicity, Marine algae Sargassum glaucescens, Apoptosis, breast cancer, colorectal cancer
Type of Study: Research | Subject: General
Received: 2017/10/23 | Accepted: 2018/06/18
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