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:: Volume 21, Issue 4 (Bimonthly 2017) ::
Feyz 2017, 21(4): 298-304 Back to browse issues page
The effect of alcoholic extracts of Arctium lappa L. and Satureja hortensis L. against Trichomonas vaginalis in vitro
Mohsen Arbabi *, Zohre Fakhrieh-Kashan, Mahdi Delavari, Mohsen Taghizadeh, Hossein Hooshyar
Department of Parasitology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, I. R. Iran. , arbabi4.mohsen@yahoo.com
Abstract:   (1267 Views)
Background: Trichomonas vaginalis infection is one of the most prevalent type of vaginitis in women. Considering the side effects of metronidazole and therapeutic properties of Arctium lappa L. and Satureja hortensis L. in traditional medicine, this study aimed to examine the anti-Trichomonas effects of Arctium lappa and Satureja hortensis alcoholic extracts in vitro.
Materials and Methods: This experimental study was conducted on T. vaginalis isolated from 1203 persons referred to five health centers in Kashan city. Five T. vaginalis isolates were cultured in a TYI-S-33 medium and were used to study the effect of Arctium lappa and Satureja hortensis extracts. The effects of different concentrations (12.5, 25, 50, 100, 200, 400, 800 and 1000 µg/mL) of plant extracts on the growth of T. vaginalis trophozoeites were studied 12, 24, and 48 h after the culture. Also, the culture media and metronidazole (0.025, 0.05, 0.1, 0.2, 0.4 µg/mL) were considered as the negative and positive controls, respectively. The effects of the extracts and drug were examined by counting the number of live and dead parasites using the trypan blue staining method.
Results: Results showed that the alcoholic extracts of Satureja hortensis and Arctium lappa had an inhibitory effect on the growth of T. vaginalis. The IC50 values of the alcoholic extracts of Satureja hortensis, Arctium lappa and metronidazole after 24 h were 190.8, 996.7 and 0.0326 µg/mL, respectively.
Conclusion: The present study shows the in vitro anti-Trichomonas effect of Arctium lappa and  Satureja hortensis extracts. The anti-Trichomonas effect of Satureja hortensis extract was higher than that of the Arctium lappa extract. Further studies are recommended to investigate the anti-Trichomonas effect of major components of these plants, especially the Satureja hortensis extract.
Keywords: Trichomonas vaginalis, Alcoholic extract, Satureja hortensis L., Arctium lappa L., In vitro
Full-Text [PDF 217 kb]   (527 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/11/3 | Accepted: 2017/07/4 | Published: 2017/10/5
References
1. Harp DF, Chowdhury I. Trichomoniasis: evaluation to execution. Eur J Obstet Gynecol Reprod Biol 2011; 157(1): 3-9.
2. Schwebke JR, Barrientes FJ. Prevalence of trichomonas vaginalis isolates with resistance to metronidazole and tinidazole. Antimicrob Agents Chemother 2006; 50(12): 4209-42.
3. Sobel JD, Nyirjesy P, Brown W. Tinidazole therapy for Metronidazole Resistant Vaginal Trichomoniasis. Clin Infect Dis 2001; 33(8): 1341-6.
4. Johnston VJ, Mabey DC. Global epidemiology and control of trichomonas vaginalis. Curr Opin Infect Dis 2008; 21(1): 56-64.
5. Kim BH, Hong SS, Kwon SW, Lee HY, Sung H, Lee IJ, et al. Diarctigenin, a Lignan Constituent from Arctium lappa, Down-Regulated Zymosan-Induced Transcription of Inflammatory Genes through Suppression of DNA Binding Ability of Nuclear Factor-kappaB in Macrophages. J Pharmacol Exp Ther 2008; 327(2): 393–401.
6. Herpburn NC. Management of cutaneous leishmaniasis. Curr Opin Infect Dis 2001; 14(2): 151-4.
7. Pirali-Kheirabadi KH, Dehghani-Samani A, Adel M, Hoseinpour F. The Effect of Essential Oil of Nigella sativa and Satureia hortensis on Promastigot Stage of Lishmania major. Armaghane danesh J 2013; 18(9): 687-98.
8. Safarnejad Tameshkel F, Nasrollahi A, Khatami Nejad MR, Rahdari P, Gholam Hossein Poor F, Kazemi Afarmejani S, et al. The Antimicrobial Effect of Methanol Extracts of Eucalyptus, Satureia Hortensis and Heracleum Glabrescens on Giardia Cysts. J Med Lab 2012; 16(2): 19-26.
9. Mahboubi M, Feizabadi MM. The Antimicrobial Activity of Thyme, Sweet Marjoram, Savory and Eucalyptus oils on Escherichia coli, Salmonella typhimurium, Aspergillus niger and Aspergillus flavus. J Medicinal Plants 2009; 2(30): 137-44.
10. Diamond LS, Harlow DR, Cunnick CC. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg 1978; 72(4): 431-2.
11. Foster S, Tyler VE. A sensible guide to the use of herbs and related remedies. 4th ed. The Havorth Herbal Press. New York; 1999. P. 98-102.
12. Fakhrieh-Kashan Z, Arbabi M, Delavari M, Hooshyar H, Taghizadeh M. In-vitro Therapeutic Effect of Allium Cepa, Oliveria Decumbens Vent and Muscari Neglectum against Trichomonas Vaginalis. J Isfahan Med Sch 2015; 32(310): 1985-92.
13. Fakhrie-Kashan Z, Arbabi M, Delavari M, Taghi-Zadeh M, Hooshyar H, Solaymani F. The effect of aqueous and alcoholic extracts of Pelarqonium roseum on the growth of Trichomonas vaginalis in vitro. Feyz 2014; 18(4): 369-75. [in Persian]
14. Cedillo-Rivera R, Chávez B, González-Robles A, Tapia A, Yépez-Mulia L. In vitro effect of nitazoxanide against Entamoeba histolytica, Giardia intestinalis and Trichomonas vaginalis trophozoites. J Eukaryot Microbiol 2002; 49(3): 201-8.
15. Calzada F, Yépez-Mulia L, Tapia-Contreras A. Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites. J Ethnopharmacol 2007; 113(2): 248-51.
16. Ahmed SA. In vitro effects of aqueous extracts of garlic (Allium sativum) and onion (Allium cepa) on Trichomonas vaginalis. Parasitolog Unit J 2010; 3: 45-54.
17. Ziaiye H, Azadbakht M, Abdollahi F, Shabankhani B. Effect of methanolic extracts of Artemisia aucheri Boiss, Zataria multiflora Boiss and Myrtus comunis L.on Trichomonas vaginalis (in vitro). J Gorgan Med Sci 2006; 8(1): 34-80.
18. Hassani S, Asghari G, Yousefi H, Kazemian A, Rafieiean M, Darani HY, et al. Effects of different extracts of Eucalyptus camaldulensis on Trichomonas vaginalis parasite in culture medium. Adv Biomed Res 2013; 2(2): 47.
19. Murray MT, Pizzorno JE. Textbook of natural medicine. Churchill living Stone. London; 1999.
20. Taran M, Rezaeian M, Izadoost M, In vitro Antitrichomonas Activity of Allium Hirtifloium (Persian shallot) in comparison with Metronidazole. Iran J Public Health 2006; 35(1): 92-4.
21. Sereshti M, Yousofi Darani H, Zebardast N, Rafiaeeyan M, Manochehri Naeeni K, Yousofi K. Effect of Ethanolic and Watery Extract of Aerial Parts of Stachys Lavandulifolia on Trichomonas. J Med Plants 2012; 41(Supple8): 159-65.
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Arbabi M, Fakhrieh-Kashan Z, Delavari M, Taghizadeh M, Hooshyar H. The effect of alcoholic extracts of Arctium lappa L. and Satureja hortensis L. against Trichomonas vaginalis in vitro. Feyz. 2017; 21 (4) :298-304
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Volume 21, Issue 4 (Bimonthly 2017) Back to browse issues page
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