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:: Volume 21, Issue 4 (Bimonthly 2017) ::
Feyz 2017, 21(4): 317-325 Back to browse issues page
Investigation of the anticancer and antioxidant activity of the brown algae (Cystoseira indica) extract against the colorectal cancer cells
Ali Taheri *, Mostafa Ghaffari, Shadab Houshmandi, Mohammad Mahdi Namavari
Department of Fisheries, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, I. R. Iran. , taherienator@gmail.com
Abstract:   (2678 Views)
Background: Nowadays, numerous studies have been conducted on the use of bioactive compounds as anti-cancer agents regarding their antioxidant activities. The current study aimed to assess the anti-cancer and anti-oxidant activities of organic and water extracts of brown algae (Cystoseira indica) collected from the shores of Chabahar, Iran.
Materials and Methods: The extraction was performed based on the method of immersion by n-hexane, ethanol, methanol, chloroform and distilled water as solvent during 24 hours. The reducing power, free radical (DPPH) scavenging activity, metal chelating activity and cytotoxicity against colorectal cancer cells were examined by the MTT test.
Results: The chloroform extract showed the best reducing power compared to the other infusions, with an average of 0.36±0.02 µg/µL. Also, chloroform extract showed the best metal chelating activity with an average of 62.18±0.86 µg/µL (P<0.05). The best free radical scavenging activity observed in the ethanol and methanol extracts with concentrations of 15.83 and 33.21 µg/µL, respectively; the inhibitory activity of methanol extracts was better than ethanol extract (P<0.05). Regarding the anti-cancer properties, methanol extract (30±1.33 µg/µL) showed the greatest effect on cancer cell death and the water extract showed the least effect (66.67±1.11 µg/µL) (P<0.05).
Conclusion: The extract of the brown algae (Cystoseira indica) can be proposed as an antioxidant and anticancer compound for preclinical and clinical studies.
Keywords: Anti-cancer, Antioxidant, Free radical, Reducing power, Cystoseira indica
Full-Text [PDF 288 kb]   (1028 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/01/30 | Accepted: 2017/07/4 | Published: 2017/10/5
1. Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry 2003; 83(4): 547-50.
2. Gamal-Eldeen AM, Ahmed EF, Abo-Zeid MA. In vitro cancer chemo preventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food Chemical Toxicol 2009; 47(6): 1378-84.
3. Sohrabipour J, Rabii R. A list of marine algae of seashores of the Persian Gulf and Oman Sea in the Hormozgan province. Iran J Bot 1999; 8(1): 131– 62. [in Persian]
4. Simmons TL, Andrianasolo E, McPhail K, Flatt P, Gerwick WH. Marine natural products as anticancer drugs. Mol Cancer Ther 2005; 4(2): 333-42.
5. Blunt JW, Copp BR, Keyzers RA. Munro MH, Prinsep MR. Marine natural products. Nat Prod Rep 2012; 29(2): 144-222.
6. Moussavou G, Kwak DH, Obiang-Obonou BW, Maranquy CA, Dinzouna-Boutamba SD, Lee DH, et al. Anticancer effects of different seaweeds on human colon and breast cancers. Mar Drugs 2014; 12(9): 4898-911.
7. Patra S, Muthuraman MS. Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich Ascites tumor cells in vivo. BMC Complement Altern Med 2013; 13(1): 331.
8. Movahedinia A, Heydari M. Antioxidant activity and total phenolic content in two alga species from the Persian Gulf in Bushehr province. Iran Int J Sci Res 2014; 3(5): 954-58.
9. Rabie R, Asadi M, Sohrabipour J, Nejadsatari T, Majd M. Morphology and anatomy algae Gracilaria salicornia in the Persian Gulf seashores (Qeshm Island). Pajouhesh Va Sazandegi 2007; 20(2): 47-53. ]in Persian]
10. Salehi P, Sonboli A, Eftekhar F, Nejad-Ebrahimi S, Yousefzadi M. Essential oil composition, antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. rigida (BOISS.) RECH. f. from Iran. Biol Pharm Bull 2005; 28(10): 1892-6.
11. Chou HJ, Kuo JT, Lin ES. Comparative antioxidant properties of water extracts from different parts of beefsteak plant (Perilla frutescens). J Food Drug Anal 2009; 17(6): 489-96.
12. Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochemistry Biophysics 1994; 315(1): 161-9.
13. Yen GC, Chen HY. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agricultural Food Chemistry 1995; 43(1): 27-32.
14. Zandi K, Farsangi MH, Nabipour I, Soleimani M, Khajeh K, Sajedi RH, et al. Isolation of a 60 kDa protein with in vitro anticancer activity against human cancer cell lines from the purple fluid of the Persian Gulf sea hare, Aplysia dactylomela. African J Biotechnol 2007; 6(11).
15. O'Hara M, Kiefer D, Farrell K, Kemper K. A review of 12 commonly used medicinal herbs. Arch Fam Med 1998; 7(6): 523-36.
16. Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol 1995; 28(1): 25-30.
17. Meir S, Kanner J, Akiri B, Philosoph-Hadas S. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agricultural Food Chemistry 1995; 43(7): 1813-9.
18. Diplock AT. Will the'good fairies' please prove to us that vitamin E lessens human degenerative disease?. Free Radic Res 1997; 27(5): 511-32.
19. Yildirim A, Mavi A, Oktay M, Kara AA, Algur ÖF, Bilaloglu V. Comparison of antioxidant and antimicrobial activities of Tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camellia sinensis) extracts. J Agricultural Food Chemistry 2000; 48(10): 5030-34.
20. Siriwardhana N, Lee KW, Kim SH, Ha JW, Jeon YJ. Antioxidant activity of Hizikia fusiformis on reactive oxygen species scavenging and lipid peroxidation inhibition. Food Sci Technol International 2003; 9(5): 339–47.
21. Srivastava A, Harish SR, Shivanandappa T. Antioxidant activity of the roots of Decalepis hamiltonii (Wight & Arn.). LWT-Food Sci Technol 2006; 39(10): 1059-65.
22. Namvar F, Mohamad R, Baharara J, Zafar-Balanejad S, Fargahi F, Rahman HS. Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum). Biomed Res Int 2013; 2013: 604787.
23. Kazłowska K, Lin HV, Chang SH, Tsai. In vitro and in vivo anticancer effects of sterol fraction from red algae Porphyra dentata. Evidence-Based Complementary Alternative Med 2013.
24. Ganesan P, Kumar CS, Bhaskar N. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. Bioresource Technol 2008; 99(8): 2717-23.
25. Turkmen N, Sari F, Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin–Ciocalteu methods. Food Chemistry 2006; 99(4): 835-41.
26. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunological Methods 1983; 65(1-2): 55-63.
27. Van de Loosdrecht AA, Beelen RH, Ossenkoppele GJ, Broekhoven MG, Langenhuijsen MM. A tetrazolium-based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia. J Immunological Methods 1994; 174(1-2): 311-20.
28. Wang SK, Liang PH, Astronomo RD, Hsu TL, Hsieh SL, Burton DR, et al. Targeting the carbohydrates on HIV-1: Interaction of Oligomannose dendrons with human monoclonal antibody 2G12 and DC-SIGN. PNAS. Chem Biodivers 2008; 195: 3690-95.
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Taheri A, Ghaffari M, Houshmandi S, Namavari M M. Investigation of the anticancer and antioxidant activity of the brown algae (Cystoseira indica) extract against the colorectal cancer cells. Feyz. 2017; 21 (4) :317-325
URL: http://feyz.kaums.ac.ir/article-1-2904-en.html

Volume 21, Issue 4 (Bimonthly 2017) Back to browse issues page
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