Background: The use of medicinal plants due to their lower side effects and the various efficient components has been increased in recent years. The aim of this study was to evaluate the antinociceptive effect of hydroalcoholic extract of green tea in male mice using the hot plate and formalin tests.

Materials and Methods: In this experimental study, 56 NMRI male mice (weight, 28±3 g) were assigned to the control and experimental groups. The experimental group received the green tea extract (75, 150 and 300 mg/kg) and 30 min after the injection, the hot plate and formalin tests were used to assess the acute and chronic pain.

Results: Results showed that hydroalcoholic extract of green tea (75mg/kg) could induce antinociception in phase 2, not in phase 1 and interphase of the formalin test. Hydroalcoholic extract of green tea (150 and 300 mg/kg) could also induce antinociception in phase 1, interphase and phase 2 of the formalin test. Moreover, hydroalcoholic extract of green tea caused a delay on the painful behaviors in hot-plate test.

Conclusion: Hydroalcoholic extract of green tea has analgesic properties and can be used as a substitute for chemical analgesic drugs.