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:: Volume 16, Issue 7 (Supplement 2013) ::
Feyz 2013, 16(7): 713-714 Back to browse issues page
Evaluation of Silver nitrate effect on horseradish peroxidase enzyme
Neda Tarbali *, Maedeh Bahavar, Nahid Einollahi, Fariba Nabatchian
Kharazmi University , ntarbali@yahoo.com
Abstract:   (6026 Views)

Background: Silver is one of the heavy metals in environment and it,s amount in the earth shell is about 0.1 g per ton. Several studies have proven silver nitrate can produce ROS (oxygen reactive species) and harm cells through a variety of mechanisms including interactions with enzymes and proteins sulphydryl groups. Major sources of Silver in surface waters is natural leaching, human activities, such as mining, manufacture of silverware and jewellery, photografic manufacturing and processing which can elevate silver levels in aquatic environments.

Materials and Methods: In This study horseradish peroxidase (HRP) activity was determinded under steady-state kinetic conditions. Incubation of the enzyme with 1-100mM silver nitrate for 1-60 min in room temperature resulted in dual effects.

Results: Progressive inhibition of the enzymatic activity was observed after 30-60 min enzyme incubation with 0.05-100 mM of Ag+. After 30 and 60 min incubation time, enzyme inhibition was (35-60%) and (55-96%), respectively. However, in low concentrations of Ag+ (0.05mM ) and 15min incubation, reverse effects was observed and enzyme activity was increased up to 14/7μM/ s.

Conclusion: Results indicated that the effect of AgNo3 on HRP is time-and concentration- dependent.

Keywords: Horseradish peroxidase enzyme, Ros, Silver nitrate, Enzyme inhibition, O-dianisidine
Full-Text [PDF 136 kb]   (1170 Downloads)    
Type of Study: Research | Subject: General
Received: 2013/03/3 | Published: 2013/03/15
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Tarbali N, Bahavar M, Einollahi N, Nabatchian F. Evaluation of Silver nitrate effect on horseradish peroxidase enzyme. Feyz. 2013; 16 (7) :713-714
URL: http://feyz.kaums.ac.ir/article-1-1823-en.html

Volume 16, Issue 7 (Supplement 2013) Back to browse issues page
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