Background: Fish as well as other aquatic animals have become an important source of protein diets. Heavy metals due to their specific physical and chemical characteristics as well as their side effects on various ecosystems are considered as a major contaminator of marine environments.This study aimed to determine the magnitude of heavy metals (Cu, Pb and Cd) in muscle tissues of fish Perca fluviatilis from Amirkolaye wetland in Guilan province.

Materials and Methods: The heavy metals were extracted from the fish muscle tissue by the wet digestion methods (nitric acid and perchloric acid). Moreover, the concentrations of the heavy metals were determined using the flame atomic absorption spectrophotometer (FAAS).

Results: The results indicated that the average concentration of heavy metals Cu, Pb and Cd in the muscle tissues of Perca fluviatilis were 0.475±0.03, 0.035±0.005 and 0.28±0.04µgg-1d.w, respectively.

Conclusion: The concentrations of heavy metals in the fish muscle tissue were lower than the allowable standards of WHO, UK (MAFF), NHMRC and FDA.