Background: In this study, the effect of methylene blue in differentiation of necrotic and vital areas of traumatic liver of rabbit was studied.

Materials and Methods: Twenty healthy rabbits were selected and divided in two equal groups randomly. Through a midline incision, rabbit's abdomen was opened. In the case group liver was traumatized for necrosis by hemostat. Then, through portal vein a blue catheter (22 G) was inserted. Sterile water was added to 0.2 cc of 2% methylen blue whose volume reached to 2 ml. Then, it was injected through the catheter. the times related to painting and discoloration were recorded. Wilcixon and linear regression analysis were used to analyze the data.

Results: The mean time of liver painting and discoloration were 17.7 ± 1.4 and 53.6 ± 3.2 in controls. In the case groups, there was no painting. There was a linear correlation between painting and discoloration times (r=0.51, P=0.012).

Conclusion: Methylen blue can be used to differentiate necrotizing liver injuries.