[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 27, Issue 5 (Bimonthly 2023) ::
Feyz Med Sci J 2023, 27(5): 493-500 Back to browse issues page
Histopathological changes following the use of thiophanate methyl fungicide in brain tissue of Wistar rats
Zahra Harsini , Seyed mohammad Hosseini * , Fereshteh Purabdolhosein
Department of Pathology, Faculty of Veterinary Medicine, Islamic Azad University, Babol Branch, Babol, Iran , dr_hosseini2323@yahoo.com
Abstract:   (653 Views)
Background and Aim: Thiophanate methyl fungicide, which is used to control the most common fungal diseases in agricultural products, causes high cytotoxic effects in cells through changes in the physiological homeostasis. The present study was conducted to evaluate the histopathological changes following the use of thiophanate methyl fungicide in the brain tissue of Wistar rats.
Methods: In this experimental study, 32 male Wistar rats were randomly assigned into 4 groups of 8, one control group and three groups receiving thiophanate methyl poison dissolved in corn oil with doses of 1328 (group A), 996 (group B) and 664 (group C) (mg/kg body weight). At the end, Nissl and hematoxylin-eosin staining was done to evaluate the damage caused in the brain tissue.
Results: Evaluations on the brain tissue showed a range of pathological changes in the cerebral cortex of group A and necrosis of neurons in all groups receiving thiophanate methyl poison, followed by a decrease in the density of neurons in the hippocampus and cerebral cortex, and the amount of this decrease in all areas of the CA2,3 area in groups A (106.40±5.12) and B (126.10±5.22) was higher than group C (161.30±6.79) (P<0.05).
Conclusion: Thiophanate methyl fungicide causes a decrease in the density of neurons in these areas in the central nervous system through damage to the cells of the cerebral cortex and the main areas of the hippocampus, which increased the severity of this damage after increasing the dose of the fungicide.
Keywords: Thiophanate methyl, Hippocampus, Histopathology, Nissl
Full-Text [PDF 558 kb]   (558 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/08/26 | Revised: 2023/12/13 | Accepted: 2023/11/18 | Published: 2023/12/10
References
1. Carisse O. Fungicides: BoD-Books on Demand; 2010. 74-69. doi:10.5772/555
2. NathPandit D, Rani U. MohitaSardana, Tricyclazole Induced Changes in Certain Haematological Indices of Channapunctatus (Bloch) as Diagnostic Biomarkers of Stress Response. Int J Pharm Sci 2021; 12(1): b97-103 doi:10.22376/ijpbs.2021.12.1.b97-103
3. SILVA JN, Monteiro NR, Antunes PA, Favareto APA. Maternal and developmental toxicity after exposure to formulation of chlorothalonil and thiophanate-methyl during organogenesis in rats. Anais da Academia Brasileira de Ciências 2020; 92 doi:10.1590/0001-3765202020191026 PMid:33206784
4. Iyer P, Makris S. Developmental and reproductive toxicology of pesticides. Hayes' Handbook of Pesticide Toxicology: Elsevier; 2010. p. 381-440.doi:10.1016/B978-0-12-374367-1.00012-4
5. Organization WH, Residues WECoP, Food FPoEoPRi, Environment t, Residues WEGoP. Pesticide Residues in Food-2006: Evaluations: World Health Organization, Food and Agriculture Organization of the United; 2007.
6. Ben Amara I, Ben Saad H, Cherif B, Elwej A, Lassoued S, Kallel C, et al. Methyl-thiophanate increases reactive oxygen species production and induces genotoxicity in rat peripheral blood. Toxicol Mechanisms Methods 2014; 24(9): 679-87. doi:10.3109/15376516.2014.961217PMid:25179310
7. Feki A, Saad HB, Jaballi I, Magne C, Boudawara O, Zeghal K, et al. Methyl thiophanate-induced toxicity in liver and kidney of adult rats: a biochemical, molecular and histopathological approach. Cellular Molecular Biol 2017; 63(2): 20-8 doi:10.14715/cmb/2017.63.2.4 PMid:28364781
8. Ghasemnejad-Berenji M, Nemati M, Pourheydar B, Gholizadeh S, Karimipour M, Mohebbi I, Jafari A. Neurological effects of long-term exposure to low doses of pesticides mixtures in male rats: Biochemical, histological, and neurobehavioral evaluations. Chemosphere 2021; 264: 128464.doi:10.1016/j.chemosphere.2020.128464 PMid:33049502
9. Lester DS, Slikker Jr W, Lazarovici P. Site-selective neurotoxicity: CRC Press; 2002. doi:10.1201/9780203219096
10. Aloizou A-M, Siokas V, Vogiatzi C, Peristeri E, Docea AO, Petrakis D, et al. Pesticides, cognitive functions and dementia: A review. Toxicol Lett 2020; 326: 31-51 doi:10.1016/j.toxlet.2020.03.005 PMid:32145396
11. Ebedy YA, Hassanen EI, Hussien AM, Ibrahim MA, Elshazly M. Neurobehavioral Toxicity Induced by Carbendazim in Rats and the Role of iNOS, Cox-2, and NF-κB Signalling Pathway. Neurochemical Res 2022:1-16 doi:10.1007/s11064-022-03581-5 PMid:35312909
12. Ahmed MS, Massoud AH, Derbalah AS, Ismail AA. Pathological and Biochemical Assesment of the Fungicide (Metalaxyl) on Rats. Egypt J Comp Pathol Clin Pathol. 2011; 24: 136-54.
13. Mumby DG, Gaskin S, Glenn MJ, Schramek TE, Lehmann H. Hippocampal damage and exploratory preferences in rats: memory for objects, places, and contexts. Learning Memory 2002; 9(2): 49-57. doi:10.1101/lm.41302 PMid:11992015 PMCid:PMC155935
14. Wallig MA, Bolon B, Haschek WM, Rousseaux CG. Fundamentals of toxicologic pathology: Academic press; 2017.
15. do Amaral LA, Farias Pereira Subtil Cavalcante AC, da Silva Fleming de Almeida T, Marques Romeiro Santos M, Candeloro Portugal L, Suzuki dos Santos B, et al. Acute and subacute (28 days) oral toxicity studies of tucum almond oil (Bactris Setosa Mart.) in mice. Drug Chemical Toxicol 2022; 45(4): 1754-60. doi:10.1080/01480545.2021.1871737 PMid:33441004
16. Suvarna KS, Layton C, Bancroft JD. Bancroft's theory and practice of histological techniques E-Book: Elsevier Health Sciences; 2018. 126-37 p.
17. Rewcastle GW, Kestell P, Baguley BC, Denny WA. Light-induced breakdown of flavone acetic acid and xanthenone analogues in solution. J National Cancer Institute 1990; 82(6): 528-9 doi:10.1093/jnci/82.6.528 PMid:2313725
18. Duncan JR, Cock ML, Scheerlinck J-PY, Westcott KT, McLean C, Harding R, Rees SM. White matter injury after repeated endotoxin exposure in the preterm ovine fetus. Pediatric Res 2002; 52(6):941-9 doi:10.1203/00006450-200212000-00021 PMid:12438674
19. Farokhcheh M, Hejazian L, Akbarnejad Z, Pourabdolhossein F, Hosseini SM, Mehraei TM, et al. Geraniol improved memory impairment and neurotoxicity induced by zinc oxide nanoparticles in male wistar rats through its antioxidant effect. Life Sci 2021; 282: 119823. doi:10.1016/j.lfs.2021.119823 PMid:34273375
20. Nobakht M, Hoseini SM, Mortazavi P, Sohrabi I, Esmailzade B, Roosh NR, Omidzahir S. Neuropathological changes in brain cortex and hippocampus in a rat model of Alzheimer's disease. Iran Biomedical J 2011; 15(1-2):51.
21. Gatman Y, Lazarovici P, Sansonetti PJ. Cellular and Molecular Mechanisms of Toxin Action: Toxins and Signal Transduction. Trends in Biochemical Sci 1998; 23(5):196 doi:10.1016/S0968-0004(97)01142-0
22. Hoda SA, Cheng E. Robbins basic pathology. 10 ed: Oxford University Press US; 2017. p. 32-5.
23. Latuszynska J, Luty S, Raszewski G, Tokarska-Rodak M, Przebirowska D, Przylepa E, et al. Neurotoxic effect of dermally-applied chlorpyrifos and cypermethrin in Wistar rats. Ann Agricultural Environmental Med 2001; 8(2).
24. Shah T, Xu J, Zou X, Cheng Y, Zhang X, Hussain Q, Gill RA. Impact of Nanomaterials on Plant Economic Yield and Next Generation. Advances in Phytonanotechnology: Elsevier; 2019. p. 203-14. doi:10.1016/B978-0-12-815322-2.00008-0
25. Costa LG, Giordano G, Guizzetti M, Vitalone A. Neurotoxicity of pesticides: a brief review. Frontiers in Bioscience-Landmark. 2008; 13(4): 1240-9 doi:10.2741/2758 PMid:17981626
26. Gök E, Deveci E. Histopathological, immunohistochemical and biochemical alterations in liver tissue after fungicide-mancozeb exposures in Wistar albino rats. Acta Cirúrgica Brasileira 2022; 37 doi:10.1590/acb370404 PMid:35766670 PMCid:PMC9239557
27. Rafika D, Fadila K, Nedjoud G, Hana B, Noureddine G, Abdelhak G. Triazole Fungicide Prosaro EC 250-induced Hematologicaland Biochemical Alterations in Male Rabbits (Oryctolagus Cuniculus). J Bioresource Manag 2022; 9(3):9.
28. Yeltekin AÇ. Effect of Fungicide Toxicity on Apoptosis, DNA Damage, and Antioxidant Enzymes in Van Fish. Pakistan J Zool 2023 doi:10.17582/journal.pjz/20210806130845
29. Thrash B, Uthayathas S, Karuppagounder SS, Suppiramaniam V, Dhanasekaran M, editors. Paraquat and maneb induced neurotoxicity. Proceedings-western Pharmacology Society; 2007: [Western Pharmacology Society]; 1998.
30. Lafon PA, Wang Y, Arango-Lievano M, Torrent J, Salvador-Prince L, Mansuy M, et al. Fungicide residues exposure and β-amyloid aggregation in a mouse model of Alzheimer's disease. Environmental Health Perspectives 2020; 128(1): 017011 doi:10.1289/EHP5550 PMid:31939705 PMCid:PMC7015540
31. Regueiro J, Olguín N, Simal-Gándara J, Suñol C. Toxicity evaluation of new agricultural fungicides in primary cultured cortical neurons. Environ Res 2015; 140: 37-44. doi:10.1016/j.envres.2015.03.013 PMid:25825129
32. Ellis WG, Semple JL, Hoogenboom ER, Kavlock RJ, Zeman FJ. Benomyl‐induced craniocerebral anomalies in fetuses of adequately nourished and protein‐deprived rats. Teratogenesis, Carcinogenesis Mutagenesis 1987; 7(4): 357-75 doi:10.1002/tcm.1770070404 PMid:2888215
33. Traina M, Fazzi P, Macrì C, Ricciardi C, Stazi A, Urbani E, Mantovani A, editors. In vivo studies on possible adverse effects on reproduction of the fungicide methyl thiophanate. J Appl Toxicol 1998; 18(4): 241-8. doi:10.1002/(SICI)1099-1263(199807/08)18:4<241::AID-AT500>3.0.CO;2-Q
34. Golub VM, Brewer J, Wu X, Kuruba R, Short J, Manchi M, et al. Neurostereology protocol for unbiased quantification of neuronal injury and neurodegeneration. Frontiers Aging Neurosci 2015; 7: 196 doi:10.3389/fnagi.2015.00196 PMid:26582988 PMCid:PMC4628120
35. Hassanen EI, Hussien AM, Hassan NH, Ibrahim MA, Mehanna S. A Comprehensive Study on the Mechanistic Way of Hexaflumuron and Hymexazol Induced Neurobehavioral Toxicity in Rats. Neurochemical Res 2022; 47(10):3051-62 doi:10.1007/s11064-022-03654-5 PMid:35773501 PMCid:PMC9470636
36. Burda JE, Sofroniew MV. Reactive gliosis and the multicellular response to CNS damage and disease. Neuron 2014; 81(2): 229-48 doi:10.1016/j.neuron.2013.12.034 PMid: 24462092 PMCid:PMC3984950
37. Morgan AM, Hassanen EI, Ogaly HA, Al Dulmani SA, Al‐Zahrani FA, Galal MK, et al. The ameliorative effect of N‐acetylcysteine against penconazole induced neurodegenerative and neuroinflammatory disorders in rats. J Biochemical Mol Toxicol 2021; 35(10): e22884. doi:10.1002/jbt.22884 PMid:34392569
38. Ahmed MS, Massoud AH, Derbalah AS, Al-Brakati A, Al-Abdawani MA, Eltahir HA, et al. Biochemical and histopathological alterations in different tissues of rats due to repeated oral dose Toxicity Cymoxanil Animals 2020; 10(12): 2205 doi:10.3390/ani10122205 PMid:33255611 PMCid:PMC7760546
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Harsini Z, Hosseini S M, Purabdolhosein F. Histopathological changes following the use of thiophanate methyl fungicide in brain tissue of Wistar rats. Feyz Med Sci J 2023; 27 (5) :493-500
URL: http://feyz.kaums.ac.ir/article-1-4963-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 27, Issue 5 (Bimonthly 2023) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4660