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:: Volume 25, Issue 4 (By Monthly 2021) ::
Feyz 2021, 25(4): 1040-1046 Back to browse issues page
Evaluation of anti-leishmaniasis activity of curcumin-loaded chitosan nanoparticles on Leishmania major and L. infantum in vitro
Atefeh Haddad , Mahdi Delavari * , Mohsen Arbabi , Iman Gardeshmeydani , Ali Salmani
, mdelavari1@gmail.com
Abstract:   (121 Views)
Background:  First-line drugs in the treatment of leishmaniasis are pentavalent compounds of antimony which are toxic and have side effects. This study aimed to investigate the anti-leishmaniasis effect of curcumin-loaded chitosan nanoparticles on Leishmania major and L. infantum in vitro.
Materials and Methods: After synthesis of chitosan nanoparticles containing curcumin zeta potential and charge of nanoparticles was determined by zeta analysis (DLS). Also, electron microscope (TEM) was used to prove the shape and size of nanoparticles. In the final stage, the efficacy of chitosan nanoparticles loaded with curcumin on Leishmania major and L. infantum was determined. For this purpose, the two species of Leishmania were cultured in RPMI 1640 medium containing 15% fetal bovine serum in plates of 48 houses and each wells of different concentrations of chitosan nanoparticles containing curcumin (40, 80, 160, 320 and 680 μg / ml) were added to the well. The number of parasites was counted after 24 and 48 hours.
Results: Transmission electron microscopy (TEM) images showed that the nanoparticle was spherical in size and 125+/-5 nm. The zeta potential of chitosan nanoparticles containing curcumin was -9.35. Counting the number of parasites showed that the synthesized nanoparticles have a favorable effect on inhibiting the growth of L. major and L. infantum. The IC50 of the mentioned nanoparticles against the promastigotic stage of Leishmania major and L. Infantum were 131.3 and 36.46, respectively.
Conclusion: Curcumin-loaded chitosan nanoparticles have anti-leishmaniasis effects and according to the obtained results can be studied in vivo condition.
 
Keywords: Chitosan nanoparticle, Curcumin, L. major, L. infantum, In vitro
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Type of Study: Research | Subject: medicine, paraclinic
Received: 2020/10/2 | Accepted: 2021/08/24 | Published: 2021/10/2
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haddad A, delavari M, arbabi M, gardeshmeydani I, salmani A. Evaluation of anti-leishmaniasis activity of curcumin-loaded chitosan nanoparticles on Leishmania major and L. infantum in vitro. Feyz. 2021; 25 (4) :1040-1046
URL: http://feyz.kaums.ac.ir/article-1-4238-en.html


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Volume 25, Issue 4 (By Monthly 2021) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
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