:: Volume 24, Issue 5 (Bimonthly 2020) ::
Feyz 2020, 24(5): 516-524 Back to browse issues page
Antibacterial effects Pulicaria gnaphalodes extract on some gram positive and negative bacteria
Yaser Jamshidi-Moghaddam , Ahmad Rajabizadeh * , Payam Khazaeli
Environmental Health Engineering Research Center, Kerman University of Medical Sciences, Kerman, I.R. Iran. , yaser.jm1366@yahoo.com
Abstract:   (691 Views)
Background: The general tendency of the community to use herbal medicines is due to prove the destructive effects of chemical drugs and environmental pollution. This study aimed to investigate the Anti-bacterial properties of P. gnaphalodes extract on some gram-positive and negative bacteria.
Materials and Methods: In this study, all of the organs of the P. gnaphalodes plant was dried in the shade in the summer of 2016 after collection. The Anti-bacterial effect of the plant was measured on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus to obtain the best extraction method. Next, 15 ml of Muller-Hinton-Agar medium was mixed with concentrations of 10 - 5.2 - 0.25 - 0.625 and 20 mg/ml of the extracts and 104 CFU /ml of the bacterium was inoculated and cultured after growing the colonies. In order to determine the MIC and MBC, the same volumes of 105 CFU/ ml were inoculated into Muller Hinton broth medium containing different concentrations of herbicide extracts. The culture medium was incubated for 24 hours at 37 ° C.
Results: Concentration of 20 mg/ml had the most effect on inhibition of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus bacteria, which was eliminated for 90, 68, 75 and 90% bacteria, respectively. MIC and MBC of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Bacillus cereus were obtained at 25, 50, 50, 70, 50, 80 and 30 and 50 mg/ml, respectively.
Conclusion: The results showed that the extract of P. gnaphalodes has anti-bacterial effects on some gram positive and negative bacteria.
Keywords: P. gnaphalodes, Plant extracts, Anti-bacterial agents, Gram-positive bacteria, Gram-negative bacteria
Full-Text [PDF 833 kb]   (128 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2019/12/10 | Accepted: 2020/10/12 | Published: 2020/12/15
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