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:: Volume 29, Issue 1 (Bimonthly 2025) ::
Feyz Med Sci J 2025, 29(1): 12-22 Back to browse issues page
Antioxidant effects of Launaea acanthodes (Boiss.) Kuntze plant on hormonal changes of testosterone in diabetic rats
Raheleh Rahbarian * , Jalal Shegofte
Department of Biology, Faculty of Basic Science, Payam-e-Noor University, Tehran, Iran , ra_rahbarian@yahoo.com
Abstract:   (411 Views)
Background and Aim: Phenolic compounds in plants, owing to their antioxidant properties, may exert modulatory effects on the hypothalamic-pituitary-gonadal axis and thus may reduce diabetes-induced hormonal imbalences and associated infertility. This study aimed to evaluate the impact of the antioxidant properties of the aqueous extract of Launaea acanthodes on testosterone hormone levels in male diabetic rats.
Methods: In this experimental study, 36 male Wistar rats were randomly divided into four groups: a healthy control group, a diabetic control group, and two diabetic groups treated with L. acanthodes aqueous extract at doses of 100 mg/kg and 300 mg/kg, respectively. Diabetes was experimentally induced via alloxan injection. The treatment groups received the plant extract orally for 30 consecutive days. At the end of the intervention, serum levels of glucose, insulin, and testosterone were measured. 
Results: On day 15, the diabetic group receiving 100 mg/kg of the extract and the diabetic group receiving 300 mg/kg (on both days 15 and 30) showed a significant reduction in blood glucose levels and a significant increase in serum insulin levels compared to the diabetic control group (P<0.05). Moreover, the 300 mg/kg dose led to a significant increase in testosterone levels on both days 15 and 30 compared to the diabetic control group (P<0.05).
Conclusion: It seems that the aqueous extract of Launaea acanthodes, through its phenolic compounds and antioxidant properties, can reduce glucose levels and increase insulin, thereby alleviating diabetes-induced oxidative stress. As a result, it may improve testosterone levels and reduce reproductive disorders in diabetic rats.
Keywords: Launaea acanthodes, Phenols, Antioxidants, Diabetes, Testosterone
Full-Text [PDF 484 kb]   (122 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2024/11/22 | Revised: 2025/04/29 | Accepted: 2025/03/12 | Published: 2025/04/22
References
1. Zaharia OP, Strassburger K, Strom A, Bönhof GJ, Karusheva Y, Antoniou S, et al. Risk of diabetes-associated diseases in subgroups of patients with recent-onset diabetes: a 5-year follow-up study. Lancet Diabetes Endocrinol. 2019; 7(9): 684-94. doi:10.1016/S2213-8587(19)30187-1 PMid:31345776
2. Harreiter J, Roden M. Diabetes mellitus–Definition, Klassifikation, Diagnose, Screening und Prävention (Update 2023). Wien Klin Wochenschr. 2023; 135(Suppl 1):7-17. doi:10.1007/s00508-023-02171-x PMid:36877241
3. Ballester J, Muñoz MC, Domínguez J, Rigau T, Guinovart JJ, Rodríguez‐Gil JE. Insulin‐dependent diabetes affects testicular function by FSH‐and LH‐linked mechanisms. J Androl. 2004; 25(5): 706-19. doi:10.1002/j.1939-4640.2004.tb02844.x PMid:15292102
4. Yaribeygi H, Atkin SL, Sahebkar A. Mitochondrial dysfunction in diabetes and the regulatory roles of antidiabetic agents on the mitochondrial function. J Cell Physiol. 2019; 234(6): 8402-10. doi:10.1002/jcp.27754 PMid: 30370538
5. Pitteloud N, Hardin M, Dwyer AA, Valassi E, Yialamas M, Elahi D, et al. Increasing insulin resistance is associated with a decrease in Leydig cell testosterone secretion in men. J Clin Endocrinol Metab. 2005; 90(5): 2636-41. doi:10.1210/jc.2004-2190 PMid: 15713718
6. Horii K-i, Watanabe G-i, Ingalls TH. Experimental diabetes in pregnant mice: prevention of congenital malformations in offspring by insulin. Diabetes. 1966; 15(3): 194-204. doi:10.2337/diab.15.3.194 PMid:5324879
7. Doustar Y, Gorjani A, Hashemi M. Effect of alloxan on pancreatic beta cells' apoptosis in rat. Med Sci J Islam Azad Univ. 2006; 16(1):29-33.
8. Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001; 50(6): 537-46. PMid:11829314
9. Moinifard M, Hedayati M. Alloxan and streptozotocin, tools for diabetes research. J Appl Exerc Physiol. 2015; 10(20):13-22.
10. Rahbarian R. The Effect of Avicennia marina Flavonoids on Bax, Bcl-2 and Stress Oxidation Indicators of Epididymis Sperm in Type 1 Diabetic Rats. J Anim Biol. 2024; 16(2):175-87.
11. Zihreh F, Nasri S, Karishchi P. The effect of quercetin on pituitary–gonadal axis, sperm parameters and testis tissue in male rats. J Sabzevar Univ Med Sci. 1970; 22(2):377-86.
12. Mahmodi Y, Yasa N. Investigation of chemical structure of Launaea Acanthodes (Bioss) O. Kontz flavonoids. Pharmacology Faculty, Tehran Univ. 1995.
13. Piazza L, Bertini S, Milany J. Extraction and structural characterization of the polysaccharide fraction of Launaea acanthodes gum. Carbohydr Polym. 2010; 79(2):449-54. doi:10.1016/j.carbpol.2009.08.030
14. Rahbarian R, Sepehri-Moghadam H, Sadoughi S. The effects of aqueous extract of Launaea acanthodes on oxidative stress parameters of red blood cells in diabetic rats. J Rafsanjan Univ Med Sci. 2016; 14(10): 865-78.
15. Jalali M, Rasouli MB, Tehranipour M, Ghayour N, Khayatzadeh J, Jannati H. Investigating the effects of hyperglycemia and the administration of the extract of Launaea acanthodes on liver functional disorders in rats. Physiol Pharmacol. 2012.
16. Rohbarian R, SepehriMoghadam H, Sadooghi SD. Effect of aqueous extract of Launaea acanthodes on testicular tissue and sperm parameters in alloxan-induced diabetic rats. Int Med Today. 2015; 21(1):21-9.
17. Tafakkor S, Sadoughi D, Rahbarian R. Effect of aqueous extract of Launaea acanthodes on DNA oxidative damage and antioxidant enzymes activities in diabetic rats testes tissue. Intern Med Today. 2017; 23(2): 149-55.
18. Sonee M, Sum T, Wang C, Mukherjee SK. The soy isoflavone, genistein, protects human cortical neuronal cells from oxidative stress. Neurotoxicology. 2004; 25(5): 885-91. doi:10.1016/j.neuro.2004.01.005 PMid:15288522
19. Rahbarian R. Effect of Crocin on Pituitary-Testicle Hormonal Axis in Alloxan-Induced Diabetic Male Rats. Jundishapur Sci Med J. 2023; 22(3):390-400.
20. Ahmadi-Noorbakhsh S. Sample size calculation for animal studies - with emphasis on the ethical principles of reduction of animal use. Res Med. 2018;42(3):144-53.
21. Ighodaro OM, Adeosun AM, Akinloye OA. Alloxan-induced diabetes, a common model for evaluating the glycemic-control potential of therapeutic compounds and plants extracts in experimental studies. Medicina. 2017;53(6):365-74. doi:10.1016/j.medici.2018.02.001 PMid:29548636
22. Behnam-Rassouli M, Ghayour N, Ghayour M, Ejtehadi M. Investigating the effects of hydro-alcoholic extract of Launaea acanthodes on the serum levels of glucose, insulin, lipids and lipoproteins in streptozotocin-induced type I diabetic rats. J Arak Univ Med Sci. 2012; 14(6):48-56.
23. Al-Dosari DI, Ahmed MM, Al-Rejaie SS, Alhomida AS, Ola MS. Flavonoid naringenin attenuates oxidative stress, apoptosis and improves neurotrophic effects in the diabetic rat retina. Nutrients. 2017; 9(10): 1161. doi:10.3390/nu9101161 PMid:29053641 PMCid:PMC5691762
24. Madhab M, Mangla C, Vijaya S, Patil DN, Joseph RA, Anuradha S, et al. Different Biological Activities Especially Antioxidant Activity of Plant-Based Functional Foods for Human Health. Int J. 2023; 10(4): 2419-23.
25. Malcangi G, Patano A, Ciocia AM, Netti A, Viapiano F, Palumbo I, et al. Benefits of natural antioxidants on oral health. Antioxidants. 2023; 12(6): 1309. doi:10.3390/antiox12061309 PMid:37372030 PMCid:PMC10295539
26. Ouamnina A, Alahyane A, Elateri I, Boutasknit A, Abderrazik M. Relationship between Phenolic Compounds and Antioxidant Activity of Some Moroccan Date Palm Fruit Varieties (Phoenix dactylifera L.): A Two-Year Study. Plants. 2024; 13(8): 1119. doi:10.3390/plants13081119
27. Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, et al. Antioxidants and human diseases. Clin Chim Acta. 2014; 436: 332-47. doi:10.1016/j.cca.2014.06.004 PMid: 24933428
28. Hur SJ, Lee SY, Kim YC, Choi I, Kim GB. Effect of fermentation on the antioxidant activity in plant-based foods. Food Chem. 2014; 160: 346-56. doi:10.1016/j.foodchem.2014.03.112 PMid: 24799248
29. Al-khawaldeh O, Al-Alami ZM, Althunibat OY, Abuamara TM, Mihdawi A, Abukhalil MH. Rosmarinic Acid Attenuates Testicular Damage via Modulating Oxidative Stress and Apoptosis in Streptozotocin-Induced Diabetic Albino Mice. Stresses. 2024; 4(3): 505-17. doi:10.3390/stresses4030033
30. Sefidgar SM, Ahmadi-Hamedani M, Javan AJ, Sani RN, Vayghan AJ. Effect of crocin on biochemical parameters, oxidative/antioxidative profiles, sperm characteristics and testicular histopathology in streptozotocin-induced diabetic rats. Avicenna J Phytomed. 2019; 9(4): 347. doi:10.22038/AJP.2019.12541 PMid: 31309076 PMCid:PMC6614399
31. Jung JI, Eun S, Lee JK, Seo YH, Bae MH, Kim R, et al. Effect of Eurycoma longifolia extract on testosterone synthesis in TM3 Leydig cells under oxidative stress. 2022.
32. El-Din H. Sayed A, Hamed M, Ismail RF. Natural antioxidants can improve microplastics-induced male reproductive impairment in the African catfish (Clarias gariepinus). Front Environ Sci. 2022; 9: 811466. doi:10.3389/fenvs.2021.811466
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Rahbarian R, shegofte J. Antioxidant effects of Launaea acanthodes (Boiss.) Kuntze plant on hormonal changes of testosterone in diabetic rats. Feyz Med Sci J 2025; 29 (1) :12-22
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Volume 29, Issue 1 (Bimonthly 2025) Back to browse issues page
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