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:: Volume 28, Issue 6 (Bimonthly 2024) ::
Feyz Med Sci J 2024, 28(6): 587-594 Back to browse issues page
Evaluation of the effect of exercise on the expression of IL-1β and SFRP5 genes in the adipose tissue of obese male Wistar rats
Sadegh Cheragh Birjandi * , Eyas Sangbari toroghi , Ali Yaghoubi , Mostafa Teymuri Kheravi
Department of Physical Education and Sport Sciences, Bojnourd Branch, Islamic Azad University, Bojnourd, Iran , s_birjandi2001@yahoo.com
Abstract:   (857 Views)
Background and Aim: Interleukin-1 beta (IL-1β) and Secreted Frizzled-related Protein 5 (SFRP5) are respectively inflammatory and anti-inflammatory proteins in adipose tissue. This study aimed to evaluate the effects of obesity and subsequent exercise on the expression of these inflammatory and anti-inflammatory genes in the adipose tissue of male rats.
Methods: In this study, 30 male Wistar rats were fed a high-fat diet (HFD) for eight weeks, while six others were fed a normal diet (ND). After this period, six obese rats and six normal diet rats were sacrificed for comparison. Then, 24 obese rats were randomly assigned into four groups (n=6 per group): obese control (OC), resistance training (RT), endurance training (ET), and high-intensity interval training (HIIT). The rats underwent 8 weeks of exercise training, 5 sessions per week. Gene expression of IL-1β and SFRP5 was measured using Real-Time PCR.
Results: IL-1β gene expression significantly decreased in the RT, ET, and HIIT groups compared to the obese control group (P<0.001). Additionally, IL-1β expression was significantly higher in the adipose tissue of rats fed the high-fat diet compared to those on a normal diet (P<0.001). Furthermore, SFRP5 gene expression was significantly higher in the RT, ET, and HIIT groups compared to the obese control group (P<0.001), while it significantly decreased in the high-fat diet group compared to the normal diet group (P<0.001). No significant difference was observed between the exercise groups (P>0.05).
Conclusion: The findings suggest that HIIT, endurance, and resistance training significantly reduce the expression of the pro-inflammatory gene IL-1β and increase the expression of the anti-inflammatory gene SFRP5 in adipose tissue. These exercise modalities may thus offer an effective strategy to mitigate obesity-related disorders.
Keywords: High-intensity interval training, Resistance training, Endurance training, Obesity, Interleukin 1 Beta, Secreted frizzled-related protein 5
Full-Text [PDF 497 kb]   (271 Downloads)    
Type of Study: Research | Subject: General
Received: 2024/08/1 | Revised: 2025/02/5 | Accepted: 2024/12/9 | Published: 2025/02/5
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Cheragh Birjandi S, Sangbari toroghi E, Yaghoubi A, Teymuri Kheravi M. Evaluation of the effect of exercise on the expression of IL-1β and SFRP5 genes in the adipose tissue of obese male Wistar rats. Feyz Med Sci J 2024; 28 (6) :587-594
URL: http://feyz.kaums.ac.ir/article-1-5213-en.html


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Volume 28, Issue 6 (Bimonthly 2024) Back to browse issues page
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