[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Articles archive::
For Reviewers::
Contact us::
Basic and Clinical Biochemistry and Nutrition
Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
:: Volume 27, Issue 6 (Bimonthly 2023) ::
Feyz 2023, 27(6): 679-687 Back to browse issues page
Human papillomavirus and Helicobacter pylori co-infection in gastric cancer patients
Mohammadreza Pourmohammad , Hosseinali Jomeh , Mohammadreza Khakzad , Jina Khayatzadeh , Elham Mokhtari Amirmajdi
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , J.khayatzadeh@mshdiau.ac.ir
Abstract:   (521 Views)
Background and Aim: Recent research has highlighted the potential interplay between human papillomavirus (HPV) and Helicobacter pylori (H. pylori) infections in gastric cancer patients. This study aimed to investigate the prevalence of H. pylori and HPV co-infection and its association with tumor characteristics in individuals diagnosed with gastric cancer.
Methods: In this descriptive study, 32 gastric cancer tissue samples and 32 healthy gastric tissue samples were collected from people referred to a hospital in Iran. DNA extraction was performed using a Biogen kit, and Real-time PCR was utilized to detect HPV and H. pylori infections through L1 and cagA gene primers, respectively.
Results: Over 50% of the cancer samples were classified as stage III. Helicobacter pylori infection was detected in 26 cancer tissues (81.2%) and 22 healthy tissues (67.8%). Additionally, four cancer tissue samples (12.5%) tested positive for HPV infection, while no HPV infection cases were observed in the control group. A significant association was found between H. pylori infection and various tumor characteristics, including staging, grade, tumor size, lymph node involvement, and histology in gastric cancer patients. Moreover, HPV infection showed a significant correlation with gastric cancer staging. However, no significant relationship was observed between the co-infection of H. pylori and HPV with gastric cancer.
Conclusion: The study results did not reveal a significant association between the co-infection of H. pylori and HPV with gastric cancer. Nevertheless, a notable relationship between H. pylori and HPV co-infection and gastric cancer staging was observed, highlighting the complexity of interactions between these infectious agents in gastric carcinogenesis.
Keywords: Helicobacter pylori, Human papillomavirus, Stomach Neoplasms, Real-time PCR
Full-Text [PDF 417 kb]   (89 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2023/10/5 | Revised: 2024/02/19 | Accepted: 2023/12/24 | Published: 2024/02/13
1. Usui Y, Taniyama Y, Endo M, Koyanagi YN, Kasugai Y, Oze I, et al. Helicobacter pylori, homologous-recombination genes, and gastric cancer. New England J Med. 2023; 388(13): 1181-90. doi:10.1056/NEJMoa2211807 PMid:36988593
2. Ahmadi Hedayati M, Khani D. Relationship of Social Risk Factors and Helicobacter pylori Infection with Pathological Characteristics of Gastric Carcinoma. Iran J Med Microbiol. 2020; 14(1): 43-30. doi:10.30699/ijmm.14.1.43
3. Ganjali A, Fakheri B A, Bahari A, Fahmideh L, valadan R. An unexplored triangle: Helicobacter pylori Infection, Inflammation and Gastric Cancer. IJCA 2021; 2(2): 62-73.
4. Khakzad MR, Saffari A, Mohamadpour N, Sankian M, Varasteh A, Salari F, et al. TLR4 and TLR2 expression in biopsy specimens from antral and corporal stomach zones in Helicobacter pylori infections. Rep Biochem Mol Biol. 2014; 3(1):29-37.
5. Esmaeilzadeh M, Yadegar A, Kafilzadeh F, Kargar M, Asadzadeh Aghdaei H. The interaction between Helicobacter pylori and autophagy: A putative mechanism involved in gastric carcinogenesis. J Microbial World. 2020; 13(4): 305-21.
6. Gottaslou R, Kazemi B, Megraud F, Eliasi H, Zargarizadeh A, Rakhshan M, et al. Value of Urease Test in the detection of Helicobacter pylori infections. Pars J Med Sci. 2022; 2(2): 6-11 doi:10.29252/jmj.2.2.3
7. Bidel Niko R, Pakzad S, Mahmoudlou R, Mahmoudzadeh L. Evaluation of the relationship between the prevalence of helicobacter pylori in the gallbladder mucosa and clinical symptoms in patients with symptomatic cholelithiasis. Studies Med Sci 2022; 33(8): 603-9 doi:10.52547/umj.33.8.603
8. Torabi R, Tebyanian H, Heiat M, Choopani A. Seroepidemiological prevalence of Helicobacter pylori in the south of Tehran, Iran. Novel Clin Med 2022;1(1):55-58. doi: 10.22034/ncm.2022.140810
9. Arif M, Syed S. Association of Helicobacter pylori with carcinoma of stomach. JPMA. The Journal of the Pakistan Medical Association. 2007; 57(7):337-41.
10. Ghotaslou R, Pirzadeh T, Esmailkhani A, Zahedi Bialvaie A, Ebrahimzadeh Leylabadlo H. Recent advances in the diagnosis of Helicobacter pylori infection. J Sabzevar Univ Med Sci 2022; 29(2): 179-94.
11. Van Der Kaaij RT, Koemans WJ, Van Putten M, Snaebjornsson P, Luijten JC, Van Dieren JM, et al. A population-based study on intestinal and diffuse type adenocarcinoma of the oesophagus and stomach in the Netherlands between 1989 and 2015. Eur J Cancer. 2020; 130: 23-31 doi:10.1016/j.ejca.2020.02.017 PMid:32171106
12. Aumpan N, Vilaichone RK, Nunanan P, Chonprasertsuk S, Siramolpiwat S, Bhanthumkomol P, et al . Predictors for development of complete and incomplete intestinal metaplasia (IM) associated with H. pylori infection: A large-scale study from low prevalence area of gastric cancer (IM-HP trial). Plos One. 2020; 15(10): e0239434.doi:10.1371/journal.pone.0239434 PMid:33002050 PMCid:PMC7529201
13. Yang H, Hu B. Immunological perspective: Helicobacter pylori infection and gastritis. Mediators Inflamm. 2022; 17(4): 2-10. doi:1155/2022/2944156 PMid:35300405 PMCid:PMC8923794
14. Moody C, Laimins L. Human papillomavirus oncoproteins: pathways to transformation. Nat Rev Cancer. 2010. 10(4): 550-60. doi:10.1038/nrc2886 PMid:20592731
15. Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet. 2013; 382(9895): 889-99. doi:10.1016/S0140-6736(13)60022-7 PMid:23618600
16. Hu Z, Ding W, Zhu D, Yu L, Jiang X, Wang X, et al. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. Clin Investig. 2015; 125(1): 425-36doi:10.1172/JCI78206 PMid:25500889 PMCid:PMC4382249
17. Kahn JA. HPV vaccination for the prevention of cervical intraepithelial neoplasia. New England J Med 2009; 361(3): 271-8.doi:10.1056/NEJMct0806938 PMid:19605832
18. Smyth EC, Verheij M, Allum W, Cunningham D, Cervantes A, Arnold D. Gastric cancer: ESMO Clinical Practice Guidelines for diagnosis, treatment and follow-up. Ann Oncol 2016; 27(4): 38-49. doi:10.1093/annonc/mdw350 PMid:27664260
19. Yang D, Shi Y, Tang Y, Yin H, Guo Y, Wen S, et al. Effect of HPV infection on the occurrence and development of laryngeal cancer: a review. J Cancer. 2019; 10(19): 44-55. doi:10.7150/jca.34016 PMid:31528209 PMCid:PMC6746124
20. Hoppe-Seyler K, Bossler F, Braun JA, Herrmann AL, Hoppe-Seyler F. The HPV E6/E7 oncogenes: key factors for viral carcinogenesis and therapeutic targets. Trends Microbiol. 2018; 26(2): 158-68. doi:10.1016/j.tim.2017.07.007 PMid:28823569
21. McBride AA, Warburton A. The role of integration in oncogenic progression of HPV-associated cancers. PLoS Pathogens. 2017; 13(4): e1006211. doi:10.1371/journal.ppat.1006211 PMid:28384274 PMCid:PMC5383336
22. Ahmadi E, Amini K, Sadeh M. Prevalence of CagA, CagT, CagE, vacA and hrgA genes in Helicobacter pylori strains isolated from patients with gastric cancer in Karaj city, 2016. Feyz Med Sci J. 2018; 21(6): 562-8.
23. Nafisi MR, Alipour Shadbad M, Rahimian GA, Karimi A. Comparison Three Methods, PCR Method, Cultyre and Rapid Urease Test to Detect Helicobacter pylori in the Gastric Biopsy. Med J Tabriz Univ Med Sci Health Services 2016; 38(5): 70-75.
24. Albatineh A, Sohrabi R, Baghi V, Ghanei Gheshlagh R. Effect of vitamin D on Helicobacter pylori infection and eradication: An updated systematic review and meta‐analysis. Novel Clin Med, 2023; 2(3): 155-162. doi: 10.22034/ncm.2023.385278.1074
25. Joharinia N, Mousavi-Nejad SA, Farhadi A, Safaei A, Hosseini SY, Sarvari J. The Frequency of High-Risk and Low-Risk Human Papillomavirus Genotypes in Different Grades of Cervical Lesions in Shiraz, South-West of Iran. Iran J Med Microbiol. 2023; 17(2): 161-6. doi:10.30699/ijmm.17.2.161
26. Abdolmaleki N, Khodabandehloo M, Ramezanzadeh R, Roshani D. No association between human papillomavirus and prostate cancer. Int J Cancer Manag. 2018; 11(4): doi:10.5812/ijcm.10049
27. Hosseinpouri P, Hejazi SH, Hadi F. 'Relationship between human papillomavirus and breast cancer in women with this cancer in Khuzestan province'. Iran J Obstetrics Gynecol Infertil. 2020; 23(8): 66-74. doi:10.22038/ijogi.2020.17297
28. Shariat A, Arzani P, Shirali M. Studying the Association between Human Papillomavirus and Prostate Cancer by Immunohistochemistry and PCR Techniques in Ahvaz Hospitals. Jundishapur Sci Med J. 2020; 19(4): 425-34. doi:10.22118/jsmj.2020.229601.2074
29. Sadeh M, Falsafi S, Amini K. The Prevalence of Papillomavirus-16 and-18 isolated from women with cervical cancer using Multiplex PCR. Yafte. 2019; 20(4): 1-7.
30. Bozdayi G, Dinc B, Avcikucuk H, Turhan N, Altay-Kocak A, Ozkan S, et al. Is Human Papillomavirus and Helicobacter pylori Related in Gastric Lesions? Clin Lab. 2019; 65(10): doi:10.7754/Clin.Lab.2019.181244
31. Yuan XY, Wang MY, Wang XY, Chang AY, Li J. Non-detection of Epstein-Barr virus and Human Papillomavirus in a region of high gastric cancer risk indicates a lack of a role for these viruses in gastric carcinomas. Genet Mol Biol. 2013; 36: 183. doi:10.1590/S1415-47572013005000018 PMid:23885199 PMCid:PMC3715283
32. de Souza CR, Almeida MC, Khayat AS, da Silva EL, Soares PC, Chaves LC, et al. Association between Helicobacter pylori, Epstein-Barr virus, human papillomavirus and gastric adenocarcinomas. World J Gastroenterol. 2018; 24(43): 4928. doi:10.3748/wjg.v24.i43.4928 PMid:30487702 PMCid:PMC6250917
33. Kamangar F, Qiao YL, Schiller JT, Dawsey SM, Fears T, Sun XD, et al. Human papillomavirus serology and the risk of esophageal and gastric cancers: Results from a cohort in a high‐risk region in China. Int J Cancer 2006; 119(3): 579-84. doi:10.1002/ijc.21871 PMid:16496409
34. Fakhraei F, Haghshenas MR, Hosseini V, Rafiei A, Naghshvar F, Alizadeh Navaei R. Detection of human papillomavirus DNA in gastric carcinoma specimens in a high-risk region of Iran. Biomedical Reports. 2016; 5(3): 371-5. doi:10.3892/br.2016.728 PMid:27588180 PMCid:PMC4998129
35. Jafari-Sales A, Shariat A, Bannazadeh-Baghi H, Baradaran B, Jafari B. Human Papillomavirus (HPV) Prevalence and E6 Protein Expression in Gastric Cancer Tissue Samples Compared with Non-malignant and Control Groups in East Azerbaijan Province, Iran. Iran J Med Microbiol. 2021; 17(1): 58-65. doi:10.30699/ijmm.17.1.58
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Pourmohammad M, Jomeh H, khakzad M, Khayatzadeh J, Mokhtari Amirmajdi E. Human papillomavirus and Helicobacter pylori co-infection in gastric cancer patients. Feyz 2023; 27 (6) :679-687
URL: http://feyz.kaums.ac.ir/article-1-5007-en.html

Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 27, Issue 6 (Bimonthly 2023) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4645