[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
AI::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 28, Issue 1 (Bimothly 2024) ::
Feyz Med Sci J 2024, 28(1): 10-16 Back to browse issues page
The effect of swimming training and liposomal BCAA supplementation on miR-133 and miR-206 gene expression in soleus muscle mitochondria of aged male rats
Amir Hajighasem * , Sara Ehsanimoghadam
Department of Physical Education, Central Tehran Branch, Islamic Azad University, Tehran, Iran , amirhajighasem8888@gmail.com
Abstract:   (1215 Views)
Background and Aim: The decline in mitochondrial biogenesis capacity with aging is a crucial factor in the development of age-related diseases. This study aimed at investigating the effects of swimming training and liposomal BCAA supplementation on the expression of miR-133 and miR-206 mitochondrial genes in the soleus muscle of aged male rats.
Methods: In this experimental study, 32 male Wistar rats (18 months old) were randomly assigned to four groups: control, exercise (swimming), supplement (BCAA nanoliposome), and combination (swimming exercise + BCAA nanoliposome supplement). Swimming training was conducted for 8 weeks in a plastic tank, starting with 25 minutes on the first day and increasing to 60 minutes by the eighth week. The water speed ranged from 4 to 10 liters per minute throughout the training protocol. Rats in the supplement and combination groups received 600 mg of BCAA nanoliposome via gavage five days a week for eight weeks. The expression of miR-133 and miR-206 genes was assessed using Real-time PCR.
Results: The mean expression levels of mitochondrial miR-133 and miR-206 genes in the soleus muscle of aged male rats in both the swimming training and combination groups were significantly higher compared to the BCAA nanoliposome supplement group and the control group (P=0.001). However, there was no significant difference in gene expression between the combination group and the swimming training group.
Conclusion: The findings of the present study show that eight weeks of swimming training and BCAA nanoliposome supplementation has a positive impact on apoptotic mechanisms in the soleus muscle of aged rats. Further research is recommended to explore these effects in more depth.
Keywords: Swimming, miRNA, BCAA Nanoliposome Supplement, Sarcopenia
Full-Text [PDF 397 kb]   (841 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/06/5 | Revised: 2024/05/1 | Accepted: 2024/02/18 | Published: 2024/03/13
References
1. Alansare A, Alford K, Lee S, Church T, Jung H C. The Effects of High-Intensity Interval Training vs. Moderate-Intensity Continuous Training on Heart Rate Variability in Physically Inactive Adults. Int J Environ Res Public Health. 2018; 15(7): 1508. doi:10.3390/ijerph15071508 PMid:30018242 PMCid:PMC6069078
2. Nystoriak MA, Bhatnagar A. Cardiovascular Effects and Benefits of Exercise. Front Cardiovasc Med. 2018;5:135. doi:10.3389/fcvm.2018.00135 PMid:30324108 PMCid:PMC6172294
3. Arisi MF, Chirico EN, Sebeny R, Muthukumaran G, Mu A, De Jonghe BC, et al. Myocardial apoptosis and mesenchymal stem cells with acute exercise. Physiol Rep. 2017; 5(11): 13297. doi:10.14814/phy2.13297 PMid:28576853 PMCid:PMC5471436
4. Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, et al. Heart disease and stroke statistics-2016 update: a report from the American Heart Association. Circulation. 2016; 133(4): e38-e360.
5. Weintraub WS, Daniels SR, Burke LE, Franklin BA, Goff Jr DC, Hayman LL, et al. Value of primordial and primary prevention for cardiovascular disease: a policy statement from the American Heart Association. Circulation. 2011; 124(8): 967-90. doi:10.1161/CIR.0b013e3182285a81 PMid:21788592
6. Sun T, Dong YH, Du W, Shi CY, Wang K, Tariq MA, et al. The role of microRNAs in myocardial infarction: from molecular mechanism to clinical application. Int J Mol Sci. 2017; 18(4):745.doi:10.3390/ijms18040745 PMid:28362341 PMCid:PMC5412330
7. Guidelines ECfP, Bax JJ, Baumgartner H, Ceconi C, Dean V, Deaton C, et al. Third universal definition of myocardial infarction. J Am College Cardiol. 2012; 60(16):1581-98. doi:10.1016/j.jacc.2012.08.001 PMid:22958960
8. Zhong N, Chen H, Zhao Q, Wang H, Yu X, Eaves AM, et al. Effects of griseofulvin on apoptosis through caspase-3- and caspase-9-dependent pathways in K562 leukemia cells: An in vitro study. Curr Ther Res Clin Exp. 2010; 71(16): 384-97. doi:10.1016/S0011-393X(10)80004-9 PMid:24688157
9. Rodríguez-Berriguete G, Galvis L, Fraile B, de Bethencourt FR, Martínez-Onsurbe P, Olmedilla G, et al. Immunoreactivity to caspase-3, caspase-7, caspase-8, and caspase-9 forms is frequently lost in human prostate tumors. Hum Pathol. 2012; 43 (2): 229-37. doi:10.1016/j.humpath.2011.04.024 PMid:21802116
10. Harada H, Hiraoka M, Kizaka S. Antitumor effect of TAT-oxygendependent degradation-caspase-3 fusion protein specifically stabilized and activated in hypoxic tumor cells. Cancer Res. 2002; 62(7): 2013-8.
11. Javid Tabrizi N, Bashiri J, Narimani Rad M. Effect of 12 weeks of treadmill aerobic training on cytochrome C and Caspase-9 gene expression in cardiac muscle of male rats. Qom Univ Med Sci J. 2017; 11(6): 1-9.
12. McMillan EM, Graham DA, Rush JW, Quadrilatero J. Decreased DNA fragmentation and apoptotic signaling in soleus muscle of hypertensive rats following 6 weeks of treadmill training. J Appl Physiol (1985). 2017; 113(7): 1048-57. doi:10.1152/japplphysiol.00290.2012 PMid:22858629
13. Marzetti E, Lawler JM, Hiona A, Manini T, Seo AY, Leeuwenburgh C. Modulation of age-induced apoptotic signaling and cellular remodeling by exercise and calorie restriction in skeletal muscle. Free Radic Biol Med. 2018; 44(2):160-8 doi:10.1016/j.freeradbiomed.2007.05.028 PMid:18191752
14. Koçtürk S, Kayatekin BM, Resmi H, Açikgöz O, Kaynak C, Ozer E. The apoptotic response to strenuous exercise of the gastrocnemius and solues muscle fibers in rats. Eur J Appl Physiol. 2017; 102 (5):515-24. doi:10.1007/s00421-007-0612-7 PMid:18030491
15. Youle RJ, Strasser A. The BCL-2 protein family: Opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 2018; 9(1): 47-59. doi:10.1038/nrm2308 PMid:18097445
16. Rastogi RP, Rajeshwar R, Sinha RP. Apoptosis: Molecular mechanisms and pathogenicity. EXCLI J. 2016; 8:155-88.
17. Lee SD, Shyu WC, Cheng IS, Kuo CH, Chan YS, Lin YM, et al. Effects of exercise training on cardiac apoptosis in obese rats. Nutr Metab Cardiovasc Dis. 2013; 23(6):566-73. doi:10.1016/j.numecd.2011.11.002 PMid:22402061
18. Pattele E, Privitera G, Simili V, Wohlgemuth SE, Aulisa L, Pahor M, et al. Multiple pathways to the same end: mechanisms of myonuclear apoptosis in sarcopenia of aging. ScientificWorldJournal. 2014; 10:340-9 doi:10.1100/tsw.2010.27 PMid:20191247 PMCid:PMC4311890
19. Viña J, Gomez-Cabrera MC, Borras C, Froio T, Sanchis-Gomar F, Martinez-Bello VE et al. Mitochondrial biogenesis in exercise and in ageing. Adv Drug Deliv Rev. 2009; 61(14):1369-14 doi:10.1016/j.addr.2009.06.006 PMid:19716394
20. Rasheva VI, Domingos PM. Cellular responses to endoplasmic reticulum stress and apoptosis. Apoptosis. 2019; 14(8): 996-1007 doi:10.1007/s10495-009-0341-y PMid:19360473
21. Abolfathiniya A, Fasihi-ramandi M, Tabanejad Z. Investigation of Niosomes for use as brucellosis vaccine. Novel Clin Med 2023; 2(2): 75-81. doi: 10.22034/ncm.2023.383601.1072
22. Liao Z, Li D, Chen Y, Li Y, Huang R, Zhu K, et al. Early moderate exercise benefits myocardial infarction healing via improvement of inflammation and ventricular remodelling in rats. J Cell Mol Med. 2019; 23(12): 8328-42. doi:10.1111/jcmm.14710 PMid:31612566 PMCid:PMC6850916
23. Kraljevic J, Marinovic J, Pravdic D, Zubin P, Dujic Z, Wisloff U, et al. Aerobic interval training attenuates remodelling and mitochondrial dysfunction in the post-infarction failing rat heart. Cardiovasc Res. 2013; 99(1): 55-64. doi:10.1093/cvr/cvt080 PMid:23554460
24. Wang GK, Zhu JQ, Zhang JT, Li Q, Li Y, He J, et al. Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans. Eur Heart J. 2014; 31(6): 659-66. doi:10.1093/eurheartj/ehq013 PMid:20159880
25. Ho TJ, Huang CC, Huang CY, Lin WT. Fasudil, a Rho-kinase inhibitor, protects against excessive endurance exercise training-induced cardiac hypertrophy, apoptosis and fibrosis in rats. Eur J Appl Physiol. 2017; 112(8): 2943-55. doi:10.1007/s00421-011-2270-z PMid:22160250
26. Pinchi E, Frati P, Aromatario M, Cipolloni L, Fabbri M, La Russa R, et al. miR‐1, miR‐499 and miR‐208 are sensitive markers to diagnose sudden death due to early acute myocardial infarction. J Cell Mol Med. 2019; 23(9): 6005-16 doi:10.1111/jcmm.14463 PMid:31240830 PMCid:PMC6714215
27. Soci UPR, Fernandes T, Barauna VG, Hashimoto NY, de Fátima Alves Mota G, Rosa KT, et al. Epigenetic control of exercise training-induced cardiac hypertrophy by miR-208. Clin Sci. 2016; 130(22): 2005-15 doi:10.1042/CS20160480 PMid:27503950
28. Wang B, Zhou R, Wang Y, Liu X, Shou X, Yang Y, et al. Effect of high-intensity interval training on cardiac structure and function in rats with acute myocardial infarct. Biomed Pharmacother. 2020; 131: 110690 doi:10.1016/j.biopha.2020.110690 PMid:32890969
29. Zhao D, Sun Y, Tan Y, Zhang Z, Hou Z, Gao C, et al. Short-Duration Swimming Exercise After Myocardial Infarction Attenuates Cardiac Dysfunction and Regulates Mitochondrial Quality Control in Aged Mice. Oxid Med Cell Longev. 2018; 2018: 4079041. doi:10.1155/2018/4079041 PMid:29849892 PMCid:PMC5925211
30. De Souza Vieira S, Antonio EL, de Melo BL, Portes LA, Montemor J, Oliveira HA, et al. Exercise training potentiates the cardioprotective effects of stem cells post-infarction. Heart Lung Circ. 2019; 28 (2):263-71. doi:10.1016/j.hlc.2017.11.005 PMid:29503239
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hajighasem A, Ehsanimoghadam S. The effect of swimming training and liposomal BCAA supplementation on miR-133 and miR-206 gene expression in soleus muscle mitochondria of aged male rats. Feyz Med Sci J 2024; 28 (1) :10-16
URL: http://feyz.kaums.ac.ir/article-1-4894-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 28, Issue 1 (Bimothly 2024) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.04 seconds with 46 queries by YEKTAWEB 4710