[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
AI::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 27, Issue 4 (Bimonthly 2023) ::
Feyz Med Sci J 2023, 27(4): 378-387 Back to browse issues page
The effect of an incremental training period on cardiomyocyte fibrosis in streptozotocin-diabetic male Wistar rats
Mehdi Fathiniya , Mohammad Fathi * , Rahim Mirnasouri
Department of Physical Education and Sport Sciences, Faculty of Humanity Sciences, Lorestan University, Khorammabad, Iran , fathi.m@lu.ac.ir
Abstract:   (1247 Views)
Background and Aim: Physical activity has an effect on the prevention and control of diabetes and its complications. The aim of the present study was to determine the effect of an incremental training period on cardiomyocyte fibrosis in streptozocin-diabetic male Wistar rats.
Methods: In this experimental study, 40 male Wistar rats (6-8 weeks) weighing 200 to 250 gr were randomly assigned into four groups: diabetes (n=10), healthy (n=10), diabetes+exercise (n=10) and healthy+exercise (n=10). The diabetes+exercise and healthy+exercise groups participated in an endurance activity program (treadmill running) for 6 weeks, 5 days a week (30 sessions). In this study, 48 hours after the completion of the protocol, rats were anesthetized and under sterile conditions, their heart tissue was isolated to evaluate the expression of collagen and transforming growth factor beta (TGF-β) genes using real-time PCR.
Results: At the end of the research protocol, a significant change was observed in weight (P₌0.0001), myocardial TGF-β level (P₌0.0001) and myocardial collagen level (P₌0.0001).
Conclusion: The findings demonstrate that diabetes increases the expression of collagen and TGF-β genes in the heart tissue of male Wistar rats, while endurance activity reduces the increased expression of these genes.
Keywords: Endurance training, TGF-β, Collagen, Diabetes
Full-Text [PDF 579 kb]   (736 Downloads)    
Type of Study: Research | Subject: General
Received: 2023/03/26 | Revised: 2023/10/30 | Accepted: 2023/07/2 | Published: 2023/10/23
References
1. Zheng Y, Ley SH, Hu FB. Global aetiology and epidemiology of type 2 diabetes mellitus and its complications. Nat Rev Endocrinol 2018; 14(2): 88-98. doi:10.1038/nrendo.2017.151 PMid:29219149
2. Jafarvand E, Ataey A, Edalati S. Epidemiology and Death Trends Due to Diabetes in Iran. Quarterly Horizon Med Sci 2021; 27(2): 198-213. doi:10.32598/hms.27.2.2764.1
3. Mezil SA, Abed BA. Complication of diabetes mellitus. Ann Rom Soc Cell Biol 2021: 1546-56.
4. Simó R, Hernández C. Treatment of diabetes mellitus: general goals, and clinical practice management. Revista Espanola de Cardiologia 2002; 55(8): 845-60. doi:10.1016/S0300-8932(02)76714-6 PMid:12199981
5. Sharma A, Mittal S, Aggarwal R, Chauhan MK. Diabetes and cardiovascular disease: inter-relation of risk factors and treatment. Futur J Pharm Sci 2020; 6(1): 1-19. doi:10.1186/s43094-021-00395-0
6. Fiuza-Luces C, Santos-Lozano A, Joyner M, Carrera-Bastos P, Picazo O, Zugaza JL, et al. Exercise benefits in cardiovascular disease: beyond attenuation of traditional risk factors. Nat Rev Cardiol 2018; 15(12): 731-43. doi:10.1038/s41569-018-0065-1 PMid:30115967
7. Asano RY, Sales MM, Browne RAV, Moraes JFVN, Júnior HJC, Moraes MR, et al. Acute effects of physical exercise in type 2 diabetes: a review. World J Diabetes 2014; 5(5): 659. doi:10.4239/wjd.v5.i5.659 PMid:25317243 PMCid:PMC4138589
8. Pinckard K, Baskin KK, Stanford KI. Effects of exercise to improve cardiovascular health. Front Cardiovasc Med 2019; 6: 69. doi:10.3389/fcvm.2019.00069 PMid:31214598 PMCid:PMC6557987
9. Wu N, Bredin SS, Jamnik VK, Koehle MS, Guan Y, Shellington EM, et al. Association between physical activity level and cardiovascular risk factors in adolescents living with type 1 diabetes mellitus: a cross-sectional study. Cardiovasc Diabetol 2021; 20(1): 1-11. doi:10.1186/s12933-021-01255-0 PMid:33712025 PMCid:PMC7955612
10. Yang R, Jia Q, Liu XF, Ma SF. Effect of genistein on myocardial fibrosis in diabetic rats and its mechanism. Molecular Med Reports 2018;17(2): 2929-36. doi:10.3892/mmr.2017.8268
11. Godarzi F, Nikbakht H, abednatanzi H, Ebrahim K, Ghazalian F. Comparison the effect of aerobic and resistance training on some oxidative parameters and TGF-β in cardiac tissue of elderly rats. Razi J Med Sci 2020; 27(3): 93-100.
12. Saadat S, Noureddini M, Mahjoubin-Tehran M, Nazemi S, Shojaie L, Aschner M, et al. Pivotal role of TGF-β/Smad signaling in cardiac fibrosis: non-coding RNAs as effectual players. Front Cardiovasc Med 2021; 7: 588347. doi:10.3389/fcvm.2020.588347 PMid:33569393 PMCid:PMC7868343
13. Samiei A, Behpour N, Tadibi V, Fathi R. Effect of Eight Weeks of Aerobic Training on Some Myocardial Fibrosis Indices in Cardiac Muscle of Diabetic Rats. Ann Appl Sport Sci 2018; 6(4): 1-8. doi:10.29252/aassjournal.6.4.1
14. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods 2001; 25(4): 402-8. doi:10.1006/meth.2001.1262 PMid:11846609
15. Howarth F, Jacobson M, Shafiullah M, Adeghate E. Long‐term effects of streptozotocin‐induced diabetes on the electrocardiogram, physical activity and body temperature in rats. Experimental Physiol 2005; 90(6): 827-35. doi:10.1113/expphysiol.2005.031252 PMid:16091403
16. Zafar M, Naqvi SN-u-H. Effects of STZ-induced diabetes on the relative weights of kidney, liver and pancreas in albino rats: a comparative study. Int J Morphol 2010; 28(1). doi:10.4067/S0717-95022010000100019
17. Pournaghi P, Sadrkhanlou RA, Hasanzadeh S, Foroughi A, editors. An investigation on body weights, blood glucose levels and pituitary-gonadal axis hormones in diabetic and metformin-treated diabetic female rats. Veterinary research forum; 2012: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
18. Mohammadi E, Fathi M, Chehel Cheraghi F, Nazari A. The effect of six weeks of endurance training and Empagliflozin consumption on heart weight and electrical changes of the heart in male Wistar rat's diabetic with STZ. Sci Magazine Yafte 2021; 23(0): 199-210.
19. Thakur V, Alcoreza N, Delgado M, Joddar B, Chattopadhyay M. Cardioprotective effect of glycyrrhizin on myocardial remodeling in diabetic rats. Biomolecules 2021; 11(4): 569. doi:10.3390/biom11040569 PMid:33924458 PMCid:PMC8069839
20. Wang X, Mu C, Mu T, Gao L, Zhao Y, Zhang Y, et al. Effects of Tongxinluo on myocardial fibrosis in diabetic rats. J Chinese Med Assoc 2016; 79(3): 130-6. doi:10.1016/j.jcma.2015.06.022 PMid:26775602
21. Wu H, Li GN, Xie J, Li R, Chen QH, Chen JZ, et al. Resveratrol ameliorates myocardial fibrosis by inhibiting ROS/ERK/TGF-β/periostin pathway in STZ-induced diabetic mice. BMC Cardiovasc Disord 2016; 16(1): 1-10. doi:10.1186/s12872-015-0169-z PMid:26750922 PMCid:PMC4707778
22. Habibian M, Khosravi M. The effect of 8 weeks regular swimming exercise on the cardiac levels of matrix mettaloproteinase-2 and transforming growth factor-β1 in diabetic rats. Iran J Diabetes Lipid Disord 2016; 15(2): 67-74.
23. Ma Y, Kuang Y, Bo W, Liang Q, Zhu W, Cai M, et al. Exercise training alleviates cardiac fibrosis through increasing fibroblast growth factor 21 and regulating TGF-β1-Smad2/3-MMP2/9 signaling in mice with myocardial infarction. Int J Mol Sci 2021; 22(22): 12341. doi:10.3390/ijms222212341 PMid:34830222 PMCid:PMC8623999
24. Liu Y, Liu X, Deng P, Ji W, Li J. Exercise effects on myocardial type I, III collagen and angiotensin II/transforming growth factor beta1/Smad2 pathway in diabetic myocardial fibrosis rats. Chinese J Tissue Engineering Res 2022; 26(26): 4173.
25. Chen X, Liu B, Yang K, Lu P, Yu H. Treadmill exercise improves the myocardial fibrosis of spontaneous type 2 diabetic mice: an exploration on the functional pathway. Chinese J Tissue Engineering Res 2022; 26(8): 1210.
26. Farzanegi P. The effect of regular swim training with two different time periods on serum levels of NO, VEGF, and TGF-β1 in diabetic male rats. Pathobiol Res 2017; 20(2): 37-48.
27. Szabó R, Karácsonyi Z, Börzsei D, Juhász B, Al-Awar A, Török S, et al. Role of exercise-induced cardiac remodeling in ovariectomized female rats. Oxid Med Cell Longev 2018; 2018. doi:10.1155/2018/6709742 PMid:29636852 PMCid:PMC5831964
28. Sakakibara M, Hirashiki A, Cheng XW, Bando Y, Ohshima K, Okumura T, et al. Association of diabetes mellitus with myocardial collagen accumulation and relaxation impairment in patients with dilated cardiomyopathy. Diabetes Res Clin Practice 2011; 92(3): 348-55. doi:10.1016/j.diabres.2011.02.023 PMid:21414680
29. Mohamad HE, Askar ME, Hafez MM. Management of cardiac fibrosis in diabetic rats; the role of peroxisome proliferator activated receptor gamma (PPAR-gamma) and calcium channel blockers (CCBs). Diabetol Metab Syndr 2011; 3(1): 1-12. doi:10.1186/1758-5996-3-4 PMid:21450068 PMCid:PMC3074550
30. Hinderer S, Schenke-Layland K. Cardiac fibrosis-A short review of causes and therapeutic strategies. Adv Drug Deliv Rev 2019; 146: 77-82. doi:10.1016/j.addr.2019.05.011 PMid:31158407
31. Li S, Liang M, Gao D, Su Q, Laher I. Changes in titin and collagen modulate effects of aerobic and resistance exercise on diabetic cardiac function. J CardiovascTranslational Res 2019; 12(5): 404-14. doi:10.1007/s12265-019-09875-4 PMid:30820865
32. Golbashi R, Gaeini A, Kordi MR, Aboutaleb N, Ghardashi Afousi A. Effect of one period of high-intensity interval training on myocardial collagen-1 and TGF-β1 and cardiac function in post ischemia-reperfusion rats. Daneshvar Med 2018; 26(2): 65-74.
33. Melo SF, Fernandes T, Baraúna VG, Matos KC, Santos AA, Tucci PJ, et al. Expression of microRNA-29 and collagen in cardiac muscle after swimming training in myocardial-infarcted rats. Cell Physiol Biochem 2014; 33(3): 657-69. doi:10.1159/000358642 PMid:24642957
34. Zheng J, Cheng J, Zheng S, Zhang L, Guo X, Zhang J, et al. Physical exercise and its protective effects on diabetic cardiomyopathy: what is the evidence? Frontiers Endocrinol 2018; 9: 729. doi:10.3389/fendo.2018.00729 PMid:30559720 PMCid:PMC6286969
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Fathiniya M, Fathi M, Mirnasouri R. The effect of an incremental training period on cardiomyocyte fibrosis in streptozotocin-diabetic male Wistar rats. Feyz Med Sci J 2023; 27 (4) :378-387
URL: http://feyz.kaums.ac.ir/article-1-4853-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 27, Issue 4 (Bimonthly 2023) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.04 seconds with 44 queries by YEKTAWEB 4710