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:: Volume 26, Issue 4 (Bimonthly 2022) ::
Feyz 2022, 26(4): 398-406 Back to browse issues page
Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model
Alireza Nikoozadeh , Roghayeh Pouzesh-Jadidi , Alireza Nourazar , Masoud Asgharpour-Arshad , Jabar Bashiri
Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran. , poozesh@iaut.ac.ir
Abstract:   (937 Views)
Background: Mitochondrial dysfunction is one of the possible mechanisms for the progression of heart disease. This study aimed to effect of eight weeks of aerobic training and curcumin supplementation on PGC1- α gene expression and cardiomyocyte mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model.
Materials and Methods: 40 male rats were randomly divided into 5 groups: healthy control, heart myocardial infarction control, aerobic exercise, supplement and exercise + supplement. The training and training+supplement groups were influenced by training on the intelligent electronic animal treadmill for 8 weeks, 5 days a week. At this time, the myocardial infarction control and supplement groups had no exercise program. First, healthy controls were killed. Expression of PGC1-α gene and mitochondrial citrate synthase using real-time PCR method, the obtained data were analyzed using Shapiro-Wilk test, two-way and one-way analysis of variance and Tukey's post hoc test.
Result: Results showed that the expression of PGC1-α and citrate synthase of cardiomyocytes in the groups trained with and without curcumin consumption is significantly higher than the supplemented and myocardial infarction control groups (P=0.001). No significant difference was observed between the two groups of exercise and exercise + supplement. The expression of both genes in the myocardial infarction control group was lower than that of healthy controls (P≤0.05).
Conclusion: Eight weeks of aerobic exercise alone and in combination with curcumin increased the expression of PGC1-α gene and citrate synthase. The combined effect of exercise and curcumin were nonsignificantly greater.
Keywords: Aerobic exercise, Curcumin, Myocardial infarction, Peroxisome proliferator activated receptor alpha, PGC1-α, Citrate synthase
Full-Text [PDF 786 kb]   (400 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/05/9 | Revised: 2022/10/8 | Accepted: 2022/07/27 | Published: 2022/09/26
References
1. Fan L, Xu H, Yang R, Zang Y, Chen J, Qin H. Combination of capsaicin and capsiate induces browning in 3T3-L1 white adipocytes via activation of the peroxisome proliferator-activated receptor γ/ β3-adrenergic receptor signaling pathways. J Agric Food Chem 2019; 67(22): 6232-40.
2. Jamali E, Asad MR, Rassouli A. Effect of eight-week endurance exercise on resistin gene expression in visceral adipose tissues in obese rats. J SSM 2017; 25(1): 20-31. [in Persian]
3. Rocha-Rodrigues S, Rodríguez A, Gouveia AM, Gonçalves IO, Becerril S, Ramírez B, et al. Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet. Life Sci 2016; 165: 100-8.
4. Kazeminasab F, Marandi SM, Ghaedi K, Safaeinejad Z, Esfarjani F, Nasr-Esfahani MH. A comparative study on the effects of high-fat diet and endurance training on the PGC-1α-FNDC5/irisin pathway in obese and nonobese male C57BL/6 mice. Appl Physiol Nutr Metab 2018; 43(7): 651-62.
5. Morton TL, Galior K, McGrath C, Wu X, Uzer G, Uzer GB, et al. Exercise increases and browns muscle lipid in high-fat diet-fed mice. Front Endocrinol 2016; 7:80
6. Gibellini L, Bianchini E, De Biasi S, Nasi M, Cossarizza A, Pinti M. Natural Compounds Modulating Mitochondrial Functions. Evid Based Complement Alternat Med 2015; 527209.
7. Halling JF, Ringholm S, Olesen J, Prats C, Pilegaard H. Exercise training protects against aging-induced mitochondrial fragmentation in mouse skeletal muscle in a PGC-1alpha dependent manner. Exp Gerontol 2017; 96: 1- 6.
8. Faheem NM, El Askary A. Neuroprotective role of curcumin on the hippocampus against the structural and serological alterations of streptozotocin-induced diabetes in Sprague Dawely rats. Iran J Basic Med Sci 2017; 20(6): 690-9.
9. Irrcher I, Adhihetty PJ, Sheehan T, Joseph AM, Hood DA. PPARgamma coactivator-1alpha expression during thyroid hormone- and contractile activity-induced mitochondrial adaptations. Am J Physiol Cell Physiol 2013; 284(6): C1669-77.
10. Iqbal S, Ostojic O, Singh K, Joseph AM, Hood DA. Expression of mitochondrial fission and fusion regulatory proteins in skeletal muscle during chronic use and disuse. Muscle & nerve 2013; 48(6): 963-70.
11. Kang C, Chung E, Diffee G, Ji LL. Exercise training attenuates aging-associated mitochondrial dysfunction in rat skeletal muscle: role of PGC-1α. Exp Gerontol 2013; 48(11): 1343-50.
12. Adhihetty PJ, Uguccioni G, Leick L, Hidalgo J, Pilegaard H, Hood DA. The role of PGC-1α on mitochondrial function and apoptotic susceptibility in muscle. Am J Physiol Cell Physiol 2019; 297(1): C217-25.
13. Dillon LM, Rebelo AP, Moraes CT. The role of PGC‐1 coactivators in aging skeletal muscle and heart. IUBMB Life 2012; 64(3): 231-41.
14. Sun J, Brown TT, Samuels DC, Hulgan T, D’Souza G, Jamieson BD, et al. The Role of Mitochondrial DNA Variation in Age-Related Decline in Gait Speed among Older Men Living With Human Immunodeficiency Virus. Clin Infect Dis 2018 22; 67(5): 778-84.
15. Dohm GL, Huston RL, Askew EW, Fleshood HL. Effects of exercise, training, and diet on muscle citric acid cycle enzyme activity. Can J Biochem 2017; 51(6): 849-54.
16. Baskaran P, Krishnan V, Ren J, Thyagarajan B. Capsaicin induces browning of white adipose tissue and counters obesity by activating TRPV1 channel‐dependent mechanisms. Br J Pharmacol 2016; 173(15): 2369-89.
17. Wu MV, Bikopoulos G, Hung S, Ceddia RB. Thermogenic capacity is antagonistically regulated in classical brown and white subcutaneous fat depots by high fat diet and endurance training in rats’ impact on whole-body energy expenditure. J Biol Chem 2014; 289(49): 34129-40.
18. Zhang YJ, Li J, Huang W, Mo GY, Wang LH, Zhuo Y, et al. Effect of electroacupuncture combined with treadmill exercise on body weight and expression of PGC-1α, Irisin and AMPK in skeletal muscle of dietinduced obesity rats (Chinese). Zhen Ci Yan Jiu 2019; 44(7): 476-80.
19. Kang YS, Kim JC, Kim JS, Kim SH. Effects of swimming exercise on serum Irisin and bone FNDC5 in rat models of high-fat diet-induced osteoporosis. J Sports Sci Med 2019; 18(4): 596- 603.
20. Norheim F, Langleite TM, Hjorth M, Holen T, Kielland A, Stadheim HK, et al. The effects of acute and chronic exercise on PGC‐1α, Irisin and browning of subcutaneous adipose tissue in humans. FEBS J 2014; 281(3): 739-49.
21. Guilford BL, Parson JC, Grote CW, Vick SN, Ryals JM, Wright DE. Increased FNDC5 is associated with insulin resistance in high fat‐fed mice. Physiol Rep 2017; 5(13): e13319.
22. Liu Z, Liu Y, Gao R, Li H, Dunn T, Wu P, et al. Ethanol suppresses PGC-1alpha expression by interfering with the cAMP-CREB pathway in neuronal cells. PLoS One 2014; 9(8): e104247
23. Yoneshiro T, Saito M. Transient receptor potential activated brown fat thermogenesis as a target of food ingredients for obesity management. Curr Opin Clin Nutr Metab Care 2013; 16(6): 625-31.
24. Ray Hamidie RD, Yamada T, Ishizawa R, Saito Y, Masuda K. Curcumin treatment enhances the effect of exercise on mitochondrial biogenesis in skeletal muscle by increasing cAMP levels. Metabolism 2015; 64(10): 1334-47.
25. Gorzi A, Ekradi s. The effect of intake duration of curcumin supplementation during strenuous endurance training on GPX activity and MDA levels of liver, heart and skeletal muscle in male Wistar rats. Sport Phys 2020; 12: 22.
26. Ren X, Gaile DP, Gong Z, Qiu W, Ge Y, Zhang C, et al. Arsenic responsive microRNAs in vivo and their potential involvement in arsenic-induced oxidative stress. Toxicol Appl Pharmacol 2015; 283(3): 198-209.
27. Lin J, Handschin C, Spiegelman BM. Metabolic control through the PGC-1 family of transcription coactivators. Cell metabolism 2015; 1(6): 361-70.
28. Yan B, Wang H, Tan Y, Fu WJCTiMC. MicroRNAs in Cardiovascular Disease: Small Molecules but Big Roles. Curr Top Med Chem 2019; 19(21): 1918-47
29. Tao L, Bei Y, Lin S, Zhang H, Zhou Y, Jiang J, et al. Exercise training protects against acute myocardial infarction via improving myocardial energy metabolism and mitochondrial biogenesis. Cell Physiol Biochem 2015; 37(1): 162-75.
30. Aftab N, Vieira A. Antioxidant activities of curcumin and combinations of this curcuminoid with other phytochemicals. Phytother Res 2010; 24(4): 500-2.
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Nikoozadeh A, Pouzesh-Jadidi R, Nourazar A, Asgharpour-Arshad M, Bashiri J. Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model. Feyz 2022; 26 (4) :398-406
URL: http://feyz.kaums.ac.ir/article-1-4609-en.html


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Volume 26, Issue 4 (Bimonthly 2022) Back to browse issues page
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