[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 1 (Bimonthly 2022) ::
Feyz 2022, 26(1): 47-54 Back to browse issues page
The effect of 8 weeks caloric restriction on PLIN5 and ATGL visceral adipose tissue and insulin resistance in type 2 diabetic male rats
Arash Alimoradi , Hasan Mateen Homaie * , Saleh Rahmati
Department of Exercise Physiology٫ Faculty of Physical Education and Sport Sciences٫ Central Tehran Branch٫ Islamic Azad University٫ Tehran٫ I.R. Iran. , hasanmatinhomaee@gmail.com
Abstract:   (201 Views)
Background: There is a close link between eating fatty foods, the prevalence of obesity and type 2 diabetes. This study aimed to evaluation the effect of 8 weeks caloric restriction on Perilipins (PLIN5) and ATGL visceral adipose tissue and insulin resistance in male diabetic rats.
Material and Methods: In this experimental study, 24 male rats were divided into healthy control, diabetic control and diabetic group with caloric restriction. In the caloric restriction group, %40 of the calorie intake was restricted and other groups received the standard food they needed freely for 8 week. The relative expression of prilipine 5 and ATGL protein by Western blotting and insulin resistance were also evaluated using Homa index. Data were analyzed by one-way ANOVA and Tukey's post hoc test.
Result: A significant difference was showed between the means of the three groups in comparing the expression of PLIN5 and ATGL proteins. Post hoc test showed a significant increase in PLIN5 and ATGL in the caloric restriction group compared to the healthy and diabetic control group (P=0.001). There was also a significant decrease in insulin and glucose levels in the caloric restriction group compared to the diabetic control group (p = 0.001).
Conclusion: Calorie restriction may prevent insulin resistance by acting on the overlying proteins and thus reducing fat accumulation.
Keywords: Caloric restriction, Perilipins, Adipose triglyceride lipase, Insulin resistance
Full-Text [PDF 371 kb]   (69 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/01/3 | Accepted: 2022/03/6 | Published: 2022/04/4
1. Mackenzie R, Maxwell N, Castle P, Brickley G, Watt P. Acute hypoxia and exercise improve insulin sensitivity (SI2*) in individuals with type 2 diabetes. Diabetes Metab Res Rev 2011; 27: 94-101.
2. Akbar S, Bellary S, Griffiths HR. Dietary antioxidant interventions in type 2 diabetes patients: a meta-analysis. British J Diabetes Vascular Disease 2011; 11: 62-8.
3. Layne AS, Nasrallah S, South MA, Howell ME, McCurry MP, Ramsey MW, et al. Impaired muscle AMPK activation in the metabolic syndrome may attenuate improved insulin action after exercise training. J Clin Endocrinol Metab 2011; 96: 1815-26.
4. Conn VS, Koopman RJ, Ruppar TM, Phillips LJ, Mehr DR, Hafdahl AR. Insulin sensitivity following exercise interventions: systematic review and meta-analysis of outcomes among healthy adults. J Prim Care Community Health 2014; 5: 211-22.
5. Cartee GD. Roles of TBC1D1 and TBC1D4 in insulin-and exercise-stimulated glucose transport of skeletal muscle. Diabetologia 2015;58:19-30
6. Shaw CS, Shepherd SO, Wagenmakers AJ, Hansen D, Dendale P, Van Loon LJ. Prolonged exercise training increases intramuscular lipid content and perilipin 2 expression in type I muscle fibers of patients with type 2 diabetes. Am J Physiol Endocrinol Metab 2012; 303: E1158-E65.
7. Paul A, Chan L, Bickel PE. The PAT family of lipid droplet proteins in heart and vascular cells. Curr Hypertens Rep 2008; 10: 461-6.
8. Bosma M, Hesselink MK, Sparks LM, Timmers S, Ferraz MJ, Mattijssen F, et al. Perilipin 2 improves insulin sensitivity in skeletal muscle despite elevated intramuscular lipid levels. Diabetes 2012; 61: 2679-90.
9. Titus AR, Ridgway EN, Douglas R, Brenes ES, Mann EK, Kooijman EE. The C-Terminus of Perilipin 3 Shows Distinct Lipid Binding at Phospholipid-Oil-Aqueous Interfaces. Membranes 2021; 11: 265.
10. Whytock K, Shepherd S, Wagenmakers A, Strauss J. Hormone sensitive lipase preferentially redistributes to perilipin-5 lipid droplets in human skeletal muscle during moderate-intensity exercise. J Physiol 2018.
11. Dubé JJ, Fleishman K, Rousson V, Goodpaster BH, Amati F. Exercise dose and insulin sensitivity: relevance for diabetes prevention. Med Sci Sports Exerc 2012; 44: 793.
12. Pruchnic R, Katsiaras A, He J, Kelley DE, Winters C, Goodpaster BH. Exercise training increases intramyocellular lipid and oxidative capacity in older adults. Am J Physiol Endocrinol Metab 2004; 287: E857-E62.
13. Zhang X, Jing S, Lin H, Sun W, Jiang W, Yu C, et al. Anti-fatigue effect of anwulignan via the NRF2 and PGC-1α signaling pathway in mice. Food Funct 2019; 10: 7755-66
14. Louche K, Badin P-M, Montastier E, Laurens C, Bourlier V, de Glisezinski I, et al. Endurance exercise training up-regulates lipolytic proteins and reduces triglyceride content in skeletal muscle of obese subjects. J Clin Endocrinol Metab 2013; 98: 4863-71.
15. MacPherson RE, Herbst EA, Reynolds EJ, Vandenboom R, Roy BD, Peters SJ. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am J Physiol Regul Integr Comp Physiol 2012; 302: R29-R36.
16. Straub BK, Stoeffel P, Heid H, Zimbelmann R, Schirmacher P. Differential pattern of lipid droplet‐associated proteins and de novo perilipin expression in hepatocyte steatogenesis. Hepatology 2008; 47: 1936-46.
17. Kim D-H, Kim S-H, Kim W-H, Moon C-R. The effects of treadmill exercise on expression of UCP-2 of brown adipose tissue and TNF-α of soleus muscle in obese Zucker rats. J Exerc Nutrition Biochem 2013; 17: 199.
18. Gallardo-Montejano VI, Saxena G, Kusminski CM, Yang C, McAfee JL, Hahner L, et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1α/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat Commun 2016; 7: 1-14.
19. Liu Y, Ni Y, Zhang W, Sun YE, Ma Z, Gu X. Antinociceptive effects of caloric restriction on post-incisional pain in nonobese rats. Sci Rep 2017; 7: 1-11.
20. Holmes A, Coppey LJ, Davidson EP, Yorek MA. Rat models of diet-induced obesity and high fat/low dose streptozotocin type 2 diabetes: effect of reversal of high fat diet compared to treatment with enalapril or menhaden oil on glucose utilization and neuropathic endpoints. J Diabetes Res 2015;2015.
21. Stuart CA, South MA, Lee ML, McCurry MP, Howell ME, Ramsey MW, et al. Insulin responsiveness in metabolic syndrome after eight weeks of cycle training. Med Sci Sports Exerc 2013; 45: 2021.
22. Qi Y, Xie M, Wei L, Hou G. Insulin resistance exacerbates lung inflammation in obese patients via PI3K/Akt signaling pathway. Eur Respiratory Soc 2019; 54: PA3343.
23. Mengeste AM, Rustan AC, Lund J. Skeletal muscle energy metabolism in obesity. Obesity 2021; 29: 1582-95.
24. Larsen S, Vigelsø A, Dandanell S, Prats C, Dela F, Helge JW. Simvastatin-induced insulin resistance may be linked to decreased lipid uptake and lipid synthesis in human skeletal muscle: the LIFESTAT study. J Diabetes Res 2018; 2018.
25. Minnaard R, Schrauwen P, Schaart G, Jorgensen JA, Lenaers E, Mensink M, et al. Adipocyte differentiation-related protein and OXPAT in rat and human skeletal muscle: involvement in lipid accumulation and type 2 diabetes mellitus. J Clin Endocrinol Metab 2009; 94: 4077-85.
26. Zhou J, Waskowicz LR, Lim A, Liao X-H, Lian B, Masamune H, et al. A liver-specific thyromimetic, VK2809, decreases hepatosteatosis in glycogen storage disease type Ia. Thyroid 2019;29:1158-67.
27. Shepherd SO, Cocks M, Tipton K, Ranasinghe AM, Barker TA, Burniston JG, et al. Sprint interval and traditional endurance training increase net intramuscular triglyceride breakdown and expression of perilipin 2 and 5. J Physiol 2013; 591: 657-75.
28. Kuramoto K, Sakai F, Yoshinori N, Nakamura TY, Wakabayashi S, Kojidani T, et al. Deficiency of a lipid droplet protein, perilipin 5, suppresses myocardial lipid accumulation, thereby preventing type 1 diabetes-induced heart malfunction. Mol Cell Biol 2014; 34: 2721-31.
29. Peters SJ, Samjoo IA, Devries MC, Stevic I, Robertshaw HA, Tarnopolsky MA. Perilipin family (PLIN) proteins in human skeletal muscle: the effect of sex, obesity, and endurance training. Appl Physiol Nutr Metab 2012; 37: 724-35.
30. Kuramoto K, Okamura T, Yamaguchi T, Nakamura TY, Wakabayashi S, Morinaga H, et al. Perilipin 5, a lipid droplet-binding protein, protects heart from oxidative burden by sequestering fatty acid from excessive oxidation. J Biol Chem 2012; 287: 23852-63.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Alimoradi A, Mateen Homaie H, Rahmati S. The effect of 8 weeks caloric restriction on PLIN5 and ATGL visceral adipose tissue and insulin resistance in type 2 diabetic male rats. Feyz. 2022; 26 (1) :47-54
URL: http://feyz.kaums.ac.ir/article-1-4536-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 1 (Bimonthly 2022) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4414