[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 25, Issue 1 (Bimonthly 2021) ::
Feyz 2021, 25(1): 724-731 Back to browse issues page
The effect of vitrified ovarian tissue autotransplantation encapsulated with hyaluronic acid hydrogen on VEGF, CD31 and CD34 gene expression in rat
Maryam Akhavan-Taheri , Mojtaba Rezazadeh Valojerdi * , Bita Ebrahimi
Department of Anatomy, Medical Science Faculty, Tarbiat Modares University, Tehran, I.R. Iran. , mr_valojerdi@modares.ac.ir
Abstract:   (350 Views)
Background: One of the problems after cryopreservation and ovarian tissue transplantation is ischemia followed by follicles mortality. In the present study, hyaluronic acid hydrogel was used to improve angiogenesis in vitrified ovarian tissue transplantation in rat.
Materials and Methods: In this experimental study, 22 adult female rats (~8 weeks old) with normal estrous cycles were ovariectomized, then their right ovaries were vitrified and warmed into two groups without hyaluronic acid hydrogel (VT) and encapsulated with hyaluronic acid hydrogel (VT+HA) was autotransplanted into the dorsal muscle. Daily vaginal monitoring was performed until re-initiation of first full oestrus cycles. The ovaries were removed at the end of the first estrus cycle and angiogenesis genes VEGF, CD31 and CD34 evaluated by real time PCR. Data were analyzed by Mann-Whitney test and the significance level was considered P<0.05.
Results: All transplants were completely successful (100% successful transplant). There was no statistically significant difference in CD34 and CD31 genes between two groups. But VEGF gene expression was significantly lower in VT+HA group (0.25±0.06) than VT group (1.00±0.05) (P<0.05). Thus, the VT group appeared more successful than the VT+HA group in expressing angiogenic genes at the end of the first estrus cycle.
Conclusion: Hyaluronic acid hydrogel does not play any effective role in the growth and improvement of angiogenesis after transplantation in the short term. But due to the angiogenic property of hyaluronic acid, increasing the expression of angiogenic genes in the VT + HA group may need more time.
Keywords: Ovarian tissue, Vitrification, Ovarian transplantation, Hyaluronic acid hydrogel, Angiogenesis
Full-Text [PDF 1497 kb]   (64 Downloads)    
Type of Study: Research | Subject: General
Received: 2020/10/9 | Accepted: 2021/02/17 | Published: 2021/04/6
1. Demeestere I, Simon P, Emiliani S, Delbaere A, Englert Y. Orthotopic and heterotopic ovarian tissue transplantation. Hum Reprod Update 2009; 15(6): 649-65.
2. Marin L, Bedoschi G, Kawahara T, Oktay KH. History, Evolution and Current State of Ovarian Tissue Auto-Transplantation with Cryopreserved Tissue: a Successful Translational Research Journey from 1999 to 2020. Reprod Sci 2020:1-8.
3. Donnez J, Dolmans M, Demylle D, Jadoul P, Pirard C, Squifflet J, et al. Restoration of ovarian function after orthotopic (intraovarian and periovarian) transplantation of cryopreserved ovarian tissue in a woman treated by bone marrow transplantation for sickle cell anaemia: case report. Hum Reprod 2006; 21(1): 183-8.
4. Oktay K. Ovarian tissue cryopreservation and transplantation: preliminary findings and implications for cancer patients. Hum Reprod Update 2001; 7(6): 526-34.
5. Amorim CA, Curaba M, Van Langendonckt A, Dolmans MM, Donnez J. Vitrification as an alternative means of cryopreserving ovarian tissue. Reprod Biomed Onlin 2011; 23(2): 160-86.
6. Damous LL, Silva SMd, Lopes RAM, Sakano CRdSB, Simões MdJ, Montero EFdS. Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning. Acta Cir Bras 2009; 24(5): 387-92.
7. Gao JM, Yan J, Li R, Li M, Yan LY, Wang TR, et al. Improvement in the quality of heterotopic allotransplanted mouse ovarian tissues with basic fibroblast growth factor and fibrin hydrogel. Hum Reprod 2013: det296.
8. Gosden R. Low temperature storage and grafting of human ovarian tissue. Mol Cell Endocrinol 2000; 163(1): 125-9.
9. Liu L, Wood G, Morikawa L, Ayearst R, Fleming C, McKerlie C. Restoration of fertility by orthotopic transplantation of frozen adult mouse ovaries. Hum Reprod 2008; 23(1): 122-8.
10. Israely T, Dafni H, Nevo N, Tsafriri A, Neeman M. Angiogenesis in ectopic ovarian xenotransplantation: Multiparameter characterization of the neovasculature by dynamic contrast‐enhanced MRI. J Magn Reson Imaging 2004; 52(4): 741-50.
11. Abir R, Stav D, Taieb Y, Gabbay-Benziv R, Kirshner M, Ben-Haroush A, et al. Novel extra cellular-like matrices to improve human ovarian grafting. J Assist Reprod Genet 2020; 1-13.
12. Shirazi-Tehrani A, Mazoochi T, Akhavan-Taheri M, Salehnia M. The effects of ovarian encapsulation with alginate hydrogel on morphology and follicular count of vitrified mouse ovary. Feyz 2019; 23(5): 467-75. [in Persian]
13. Gao J, Huang Y, Li M, Zhao H, Zhao Y, Li R, et al. Effect of Local Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor on Subcutaneously Allotransplanted Ovarian Tissue in Ovariectomized Mice. PLoS One 2015; 10(7): e0134035.
14. Nepomnyashchaya EM, Ulianova EP, Sagakyants AB, Novikova IA, Vashchenko LN, Ausheva TV, et al. Factors of angiogenesis (VEGF and CD34) in primary and recurrent soft tissue sarcomas. Am Soc Clin Oncol 2020.
15. Fraser J, Laurent T, Laurent U. Hyaluronan: its nature, distribution, functions and turnover. J. Intern Med 1997; 242(1): 27-33.
16. Sattar A, Rooney P, Kumar S, Pye D, West DC, Scott I, et al. Application of angiogenic oligosaccharides of hyaluronan increases blood vessel numbers in rat skin. JID 1994; 103(4): 576-9.
17. Kagawa N, Silber S, Kuwayama M. Successful vitrification of bovine and human ovarian tissue. Reprod Biomed Online 2009; 18(4): 568-77.
18. Akhavan Taheri M, Rezazadeh Valojerdi M, Ebrahimi B. Intramuscular autotransplantation of vitrified rat ovary encapsulated with hyaluronic acid hydrogel. Biopreserv Biobank 2016; 14(2): 114-21.
19. Liebermann J, Nawroth F, Isachenko V, Isachenko E, Rahimi G, Tucker MJ. Potential importance of vitrification in reproductive medicine. Reprod Bio Online 2002; 67(6): 1671-80.
20. Fathi R, Valojerdi MR, Salehnia M. Effects of different cryoprotectant combinations on primordial follicle survivability and apoptosis incidence after vitrification of whole rat ovary. Cryo Letters 2013; 34(3): 228-38.
21. Posillico S, Kader A, Falcone T, Agarwal A. Ovarian tissue vitrification: Modalities, challenges and potentials. Curr Womens Health Rev 2010; 6(4): 352-66.
22. Youm HW, Lee JR, Lee J, Jee BC, Suh CS, Kim SH. Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified–warmed ovarian tissue survival. Hum Reprod 2014; 29(4): 720-30.
23. Wang L, Ying YF, Ouyang YL, Wang JF, Xu J. VEGF and bFGF increase survival of xenografted human ovarian tissue in an experimental rabbit model. J Assist Reprod Genet 2013; 30(10): 1301-11.
24. Tavana S, Azarnia M, Valojerdi MR, Shahverdi A. Hyaluronic acid-based hydrogel scaffold without angiogenic growth factors enhances ovarian tissue function after autotransplantation in rats. Biomed Mater 2016; 11(5): 055006.
25. Wu D, Lei Y, Tong Y, Tang F, Qian Y, Zhou Y. Angiogenesis of the Frozen‐Thawed Human Fetal Ovarian Tissue at the Early Stage after Xenotransplantation and the Positive Effect of Salviae miltiorrhizae. Anat Rec 2010; 293(12): 2154-62.
26. Soleimani R, Heytens E, Oktay K. Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants. PLoS One 2011; 6(4): e19475.
27. Redmer DA, Doraiswamy V, Bortnem BJ, Fisher K, Jablonka-Shariff A, Grazul-Bilska AT, et al. Evidence for a role of capillary pericytes in vascular growth of the developing ovine corpus luteum. Biol Reprod 2001; 65(3): 879-89.
28. Holmberg L, Iversen OE, Rudenstam CM, Hammar M, Kumpulainen E, Jaskiewicz J, et al. Increased risk of recurrence after hormone replacement therapy in breast cancer survivors. J Natl Cancer Inst 2008; 100(7): 475-82.
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Akhavan-Taheri M, Rezazadeh Valojerdi M, Ebrahimi B. The effect of vitrified ovarian tissue autotransplantation encapsulated with hyaluronic acid hydrogen on VEGF, CD31 and CD34 gene expression in rat. Feyz. 2021; 25 (1) :724-731
URL: http://feyz.kaums.ac.ir/article-1-4242-en.html

Volume 25, Issue 1 (Bimonthly 2021) Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.03 seconds with 30 queries by YEKTAWEB 4319