History and Objectives: Considering the incidence of ulcer infection within incision area and its complications and a similar condition in perforated and gangrenous appendicitis and the efficacy of local application of antibiotics in reduction of infection, this study was carried out to evaluate the effect of ampicillin washing in incision area.

Materials and Methods: The clinical trial strategy of this study was performed on two groups for patients incised with McBorni method. All of the patients received intravenous injection of metronidazole and gentamicin one day before operation and for 7 consecutive days therefore received ciprofloxacin and were followed up for a period of 1 month. For healing, control group was washed with normal saline and experimental group washed with saline solution and ampicillin.

Results: A total of 74 patients in two groups (n=37) were studied and they were alike regarding age and gender. The incidence rate in control and experimental group was 13.5% and 10.8% respectively that was non-significant statistically. In addition, age, gender and type of complication played no role in infection. Meanwhile, a thickness greater than 2cm for subcutaneous tissue can increase the incidence rate.

Conclusion: It is concluded that ampicillin washing in comparison to saline itself can not affect the incidence of infection. It is recommended to perform more studies on ulcers with a subcutaneous thickness greater than 2cm.