[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
::
Biochemistry and Nutrition in Metabolic Diseases
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 22, Issue 2 (Bimonthly 2018) ::
Feyz Med Sci J 2018, 22(2): 206-213 Back to browse issues page
Study of drug resistance of Staphylococcus aurous and Pseudomonas aeruginosa strains isolated from environmental samples of Hamadan educational hospitals in 2017 using disk diffusion and minimum inhibitory concentration
Masoume Einabadi , Fahimeh Abdolerahmani , Rasoul Yousefi Mashoof , Hossein Vazini , Ali Shakerimoghaddam , Azad Khaledi , Ahmad Piroozmand , Pezhman Karami *
Department of Microbiology, Faculty of Medicine, Medical Sciences University, Hamadan, I. R. Iran. , pezhmankarami@gmail.com
Abstract:   (5198 Views)
Background: Staphylococcus aureus and Pseudomonas aeruginosa are the most important bacteria causing the nosocomial infections, which are resistant to most of the antibiotics. The aim of this study was to evaluate the drug resistance of S. aureus and P. aeruginosa strains isolated from environmental samples of Hamedan educational hospitals using disk diffusion and minimum inhibitory concentration (MIC) methods.
Materials and Methods: In this descriptive cross-sectional study, 400 samples were collected from Hamedan educational hospitals. To assess the antibiotic susceptibility of 10 common antibiotics, the agar dilution (Kirby-Bauer) method was used. Also, to determine the MIC of S. aureus and P. aeruginosa, vancomycin and ciprofloxacin antibiotics were used.
Results: From a total of 400 samples, 39 (9.7%) isolates were P. aeruginosa and 28 (7%) were S. aureus. Staphylococcus aureus showed the highest resistance to ofloxacin (82.1%) and the highest drug resistance to P. aeruginosa was related to meropenem (82%). Also, the highest MIC and maximum bactericidal concentration (MBC) for S. aureus to vancomycin were 128 and 256, respectively. In P. aeruginosa, the highest MIC and MBC to ciprofloxacin were 128 and 256, respectively.
Conclusion: Staphylococcus aureus and P. aeruginosa showed the highest resistance to ofloxacin and meropenem, respectively. Considering the rapid increase of antibiotic resistance, accurate evaluation of the antibiotic resistance pattern of the bacteria is required.
Keywords: Antibiotic resistance, Staphylococcus aureus, Pseudomonas aeruginosa
Full-Text [PDF 246 kb]   (1723 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2017/12/23 | Revised: 2018/06/7 | Accepted: 2018/02/17 | Published: 2018/02/17
References
1. Bennett JE, Dolin R, Blaser MJ. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases E-Book. Elsevier Health Sciences; 2014.
2. Gravel D, Matlow A, Ofner-Agostini M, Loeb M, Johnston L, Bryce E, et al. A point prevalence survey of health care–associated infections in pediatric populations in major Canadian acute care hospitals. Am J Infect Control 2007; 35(3): 157-62.
3. Klevens RM, Edwards JR, Richards Jr CL, Horan TC, Gaynes RP, Pollock DA, et al. Estimating health care-associated infections and deaths in US hospitals, 2002. Public Health Rep 2007; 122(2): 160-6.
4. Saghi H, Bahador A, Khaledi A, Kachoei RA, Dastjerdi FA, Esmaeili D. Antibacterial effects of Origanum vulgare essence against multidrug-resistant Acinetobacter baumannii isolated from selected hospitals of Tehran, Iran. Avicenna J Clin Microb Infec 2015; 2(1).
5. Falahi J, Khaledi A, Alikhani MY, Taghipour A, Jamehdar SA, Honarmand M, et al. Prevalence of Nosocomial Infection in Different Wards of Ghaem Hospital, Mashhad. Avicenna J Clin Microb Infec 2017; 4(2).
6. Goossens H, Ferech M, Vander Stichele R, Elseviers M, Group EP. Outpatient antibiotic use in Europe and association with resistance: a cross-national database study. Lancet 2005; 365(9459): 579-87.
7. Ferber D. Livestock feed ban preserves drugs' power. Science 2002; 295(5552): 27-8.
8. Khademi F, Ghanbari F, Mellmann A, Najafzadeh MJ, Khaledi A. Phylogenetic relationships among Staphylococcus aureus isolated from clinical samples in Mashhad, Iran. J Infect Public Health 2016; 9(5): 639-44.
9. Khazaei S, Pourtahmaseby P, Kanani M, Madani SH, Malekianzadeh E. Staphylococcus Aureus Resistance to Vancomycin: A Six Years Survey. Med J Tabriz Uni Med Sci Health Service 2013; 35(5): 40-5. [In Persian]
10. Hosseini SMJ, Naeini NS, Khaledi A, Daymad SF, Esmaeili D. Evaluate the Relationship Between Class 1 Integrons and Drug Resistance Genes in Clinical Isolates of Pseudomonas aeruginosa. Open Microbiol J 2016; 10: 188.
11. Aloush V, Navon-Venezia S, Seigman-Igra Y, Cabili S, Carmeli Y. Multidrug-resistant Pseudomonas aeruginosa: risk factors and clinical impact. Antimicrob Agent Chemother 2006; 50(1): 43-8.
12. Hirsch EB, Tam VH. Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev Pharmacoecon Outcomes Res 2010; 10(4): 441-51.
13. Olaitan AO, Morand S, Rolain JM. Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria. Front Microbiol 2014; 5: 643.
14. Rodloff A, Bauer T, Ewig S, Kujath P, Müller E. Susceptible, intermediate, and resistant–the intensity of antibiotic action. Deutsches Ärzteblatt International 2008; 105(39): 657.
15. Zamani S, Nasiri MJ, Khoshgnab BN, Ashrafi A, Abdollahi A. Evaluation of antimicrobial resistance pattern of nosocomial and community bacterial pathogens at a teaching hospital in Tehran, Iran. Acta Med Iranica 2014; 52(3): 182.
16. Ahmadishoar SH NM, Amirmozafari N. Study the susceptibility of S.aureus strains isolated from clinical specimens to vancomycin using E-test in Tabriz. J Tabriz Uni Med Sci.2008;30(2):17-23.
17. Vanhoof R, Godard C, Content J, Nyssen H. Detection by polymerase chain reaction of genes encoding aminoglycoside-modifying enzymes in methicillin-resistant Staphylococcus aureus isolates of epidemic phage types. J Med Microbiol 1994; 41(4): 282-90.
18. Hauschild T, Sacha P, Wieczorek P, Zalewska M, Kaczyńska K, Tryniszewska E. Amino‌glyco‌sides resistance in clinical isolates of Staphylo‌coccus aureus from a University Hospital in Bialystok, Poland. Folia Histochem Cytobiol 2008; 46(2): 225-8.
19. Fatholahzadeh B, Emaneini M, Gilbert G, Udo E, Aligholi M, Modarressi MH, et al. Staphylo‌coccal cassette chromosome mec (SCC mec) analysis and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) isolates in Tehran, Iran. Microb Drug Resist 2008; 14(3): 217-20.
20. Alghaithy A, Bilal N, Gedebou M, Weily A. Nasal carriage and antibiotic resistance of Staphylococcus aureus isolates from hospital and non-hospital personnel in Abha, Saudi Arabia. Trans R Soc Trop Med Hyg 2000; 94(5): 504-7.
21. Al-Marjani M, Kadhim A, Kinani Y. Ciprofloxacin resistance in Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients in Baghdad. Int J Pharm Sci Res 2015; 6: 382-5.
22. Bokaeian M, Fakheri BA, Nejad NM, Hassansahian M, Saeidi S. The Survey of Withani somnifera Extraction against Resistant Strains of Pseudomonas aeruginosa Bacteria to Selective Antibiotics. J Med Bacteriol 2015; 4(1-2): 53-7.
23. Mardaneh J, Ahmadi K, Jahan Sepas A. Determination antimicrobial resistance profile of Pseudomonas aeruginosa strains isolated from hospitalized patients in Taleghani Hospital (Ahvaz, Iran) from 2011-2012. J Fasa Univ Med Sci 2013; 3(3): 188-93.
24. Rajabpour M, Arabestani M R, Yousefi mashof R, Alikhani M Y. MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan. Iran J Med Microbial 2013; 7 (3):18-25.
25. Mitiku M, Ali S, Kibru G. Antimicrobial drug resistance and disinfectants susceptibility of Pseudomonas aeruginosa isolates from clinical and environmental samples in Jimma University specialized hospital, Southwest Ethiopia. Am J Biomed Life Sci 2014; 2: 40-5.
26. Mohajeri P. Antibiotic susceptibility and resistance patterns of pseudomonas aeruginosa strains isolated from different clinical specimens in patients referred to the teaching hospitals in Kermanshah (2001-2). J Kermanshah Univ Med Sci 2004; 7(4).
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Einabadi M, Abdolerahmani F, Yousefi Mashoof R, Vazini H, shakerimoghaddam A, Khaledi A, et al . Study of drug resistance of Staphylococcus aurous and Pseudomonas aeruginosa strains isolated from environmental samples of Hamadan educational hospitals in 2017 using disk diffusion and minimum inhibitory concentration . Feyz Med Sci J 2018; 22 (2) :206-213
URL: http://feyz.kaums.ac.ir/article-1-3496-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 22, Issue 2 (Bimonthly 2018) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.05 seconds with 46 queries by YEKTAWEB 4660