[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing Sources::
Guide for Authors::
Online Submission::
Ethics::
Articles archive::
For Reviewers::
Contact us::
AI::
::
Basic and Clinical Biochemistry and Nutrition
..
DOAJ
..
CINAHL
..
EBSCO
..
IMEMR
..
ISC
..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
enamad
..
:: Volume 20, Issue 6 (Bimonthly 2017) ::
Feyz Med Sci J 2017, 20(6): 501-508 Back to browse issues page
Effect of eicosapentaenoic acid on insulin resistance genes in granulosa cells of patients with polycystic ovarian syndrome
Akbar Heidari , Mohammad Nouri , Mahzad Sadaghiani , Esmat Aghadavod *
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I. R. Iran. , Aghadavod@kaums.ac.ir
Abstract:   (5171 Views)

Background: Polycystic ovarian syndrome (PCOS) often induces reduced ovulation, infertility and insulin resistance. One of the most influential inducer factors in disease is peroxisome proliferator-activated receptor gamma (PPARγ) a member of the nuclear receptor superfamily, which has a variety of physiological functions. PPARγ agonists are used for the treatment of insulin resistance, hyperandrogenism and ovarian dysfunction in PCOS patients. Fatty acids like eicosapentaenoic acid (EPA) and their metabolites are natural ligands for PPARγ and a good option for regulation of PPARγ gene expression.

Materials and Methods: This case-control study was carried out on 30 women with PCOS referred to the Tabriz-Alzahra fertility center for in vitro fertilization (IVF). Granulosa cells collected from follicular fluid of these individuals were cultured in vitro. Gene expression of PPARγ, insulin-like growth factor 1(IGF-1) and cyclooxygenase-2 were examined on cultured granulosa cells in culture medium and EPA–mediated culture media.

Results: The PPARγ gene expression was increased in EPA-treated medium with a maximum expression at a concentration of 100 mmol in 48 hours. On this basis, increment of PPARγ expression lead to the increased gene expression involved in the steroidogenesis, (i.e. IGF-1). However, the exptression of both genes were decreased in PCOS and the control medium.

Conclusion: In this study, we observed that different concentrations of EPA increased PPARγ gene expression.

Keywords: Polycystic ovary syndrome, IGF-1, PPARγ, EPA
Full-Text [PDF 242 kb]   (1462 Downloads)    
Type of Study: Research | Subject: General
Received: 2017/01/1 | Revised: 2017/07/24 | Accepted: 2017/01/1 | Published: 2017/01/1
References
1. Brazert M, Pawelczyk LA. Insulin-like growth factor-1 isoforms in human ovary. Preliminary report on the expression of the IGF-1 gene in PCOS patients and healthy controls. Ginekol Pol 2015; 86(12): 890-5.
2. Rovito D, Giordano C, Plastina P, Barone I, De Amicis F, Mauro L, et al. Omega-3 DHA- and EPA-dopamine conjugates induce PPARgamma-dependent breast cancer cell death through autophagy and apoptosis. Biochim Biophys Acta 2015; 1850(11): 2185-95.
3. Sharma S, Sharma PM, Mistry DS, Chang RJ, Olefsky JM, Mellon PL, et al. PPARG regulates gonadotropin-releasing hormone signaling in LbetaT2 cells in vitro and pituitary gonadotroph function in vivo in mice. Biol Reprod 2011; 84(3): 466-75.
4. Nadjarzadeh A, Dehghani-Firouzabadi R, Daneshbodi H, Lotfi MH, Vaziri N, Mozaffari-Khosravi H. Effect of Omega-3 Supplementation on Visfatin, Adiponectin, and Anthropometric Indices in Women with Polycystic Ovarian Syndrome. J Reprod Infertil 2015; 16(4): 212-20.
5. Lalia AZ, Lanza IR. Insulin-Sensitizing Effects of Omega-3 Fatty Acids: Lost in Translation? Nutrients 2016; 8(6.(
6. Vargas ML, Almario RU, Buchan W, Kim K, Karakas SE. Metabolic and endocrine effects of long-chain versus essential omega-3 polyunsaturated fatty acids in polycystic ovary syndrome. Metabolism 2011; 60(12): 1711-8.
7. Kokosar M, Benrick A, Perfilyev A, Fornes R, Nilsson E, Maliqueo M, et al. Epigenetic and Transcriptional Alterations in Human Adipose Tissue of Polycystic Ovary Syndrome. Sci Rep 2016; 6: 22883.
8. Hasan AU, Ohmori K, Konishi K, Igarashi J, Hashimoto T, Kamitori K, et al. Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARgamma mediated pathways in 3T3-L1 adipocytes. Mol Cell Endocrinol 2015; 406: 10-8.
9. Aghadavod E, Zarghami N, Farzadi L, Zare M, Barzegari A, Movassaghpour AA, et al. Isolation of granulosa cells from follicular fluid; applications in biomedical and molecular biology experiments. Adv Biomed Res 2015; 4: 250.
10. Igoillo-Esteve M, Marselli L, Cunha DA, Ladriere L, Ortis F, Grieco FA, et al. Palmitate induces a pro-inflammatory response in human pancreatic islets that mimics CCL2 expression by beta cells in type 2 diabetes. Diabetologia 2010; 53(7): 1395-405.
11. Anderson EJ, Thayne KA, Harris M, Shaikh SR, Darden TM, Lark DS, et al. Do fish oil omega-3 fatty acids enhance antioxidant capacity and mitochondrial fatty acid oxidation in human atrial myocardium via PPARgamma activation? Antioxid Redox Signal 2014; 21(8): 1156-63.
12. Rafraf M, Mohammadi E, Asghari-Jafarabadi M, Farzadi L. Omega-3 fatty acids improve glucose metabolism without effects on obesity values and serum visfatin levels in women with polycystic ovary syndrome. J Am Coll Nutr 2012; 31(5): 361-8.
13. Elis S, Freret S, Desmarchais A, Maillard V, Cognie J, Briant E, et al. Effect of a long chain n-3 PUFA-enriched diet on production and reproduction variables in Holstein dairy cows. Anim Reprod Sci 2016; 164: 121-32.
14. Gdula-Argasinska J, Czepiel J, Toton-Zuranska J, Wolkow P, Librowski T, Czapkiewicz A, et al. n-3 Fatty acids regulate the inflammatory-state related genes in the lung epithelial cells exposed to polycyclic aromatic hydrocarbons. Pharmacol Rep 2016; 68(2): 319-28.
15. Bhaswant M, Poudyal H, Brown L. Mechanisms of enhanced insulin secretion and sensitivity with n-3 unsaturated fatty acids. J Nutr Biochem 2015; 26(6): 571-84.
16. Castillero E, Lopez-Menduina M, Martin AI, Villanua MA, Lopez-Calderon A. Comparison of the effects of the n-3 polyunsaturated fatty acid eicosapentaenoic and fenofibrate on the inhibitory effect of arthritis on IGF1. J Endocrinol 2011; 210(3): 361-8.
17. Damsgaard CT, Harslof LB, Andersen AD, Hellgren LI, Michaelsen KF, Lauritzen L. Fish oil supplementation from 9 to 18 months of age affects the insulin-like growth factor axis in a sex-specific manner in Danish infants. Br J Nutr 2016; 115(5): 782-90.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Heidari A, Nouri M, Sadaghiani M, Aghadavod E. Effect of eicosapentaenoic acid on insulin resistance genes in granulosa cells of patients with polycystic ovarian syndrome. Feyz Med Sci J 2017; 20 (6) :501-508
URL: http://feyz.kaums.ac.ir/article-1-3223-en.html


Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 20, Issue 6 (Bimonthly 2017) Back to browse issues page
مجله علوم پزشکی فیض Feyz Medical Sciences Journal
Persian site map - English site map - Created in 0.03 seconds with 46 queries by YEKTAWEB 4710