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:: Volume 20, Issue 2 (Bimonthly 2016) ::
Feyz 2016, 20(2): 147-156 Back to browse issues page
Antioxidant activity, total flavonoid and total phenolic contents of extracts taken from aerial parts of Ballota platyloma using three different methods: percolation, ultrasonic and polyphenolic fraction
Bagher Seyedalipour * , Ali Hasani , Mohammad Ali Ebrahimzadeh , Ali Taravati
Department of Cellular and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, I. R. Iran. , b.alipour81@gmail.com
Abstract:   (4221 Views)

Background: The genus Ballota L. (BL) belongs to the Labiateae family spread throughout the world.  Plants of this genus possess anti oxidant, anti cancer, anti microbial and anti inflammatory properties. This study aimed to investigate the antioxidant activities of extract taken from aerial parts of Ballota platyloma Rech. f. using percolation, ultrasonic and polyphenol fraction methods.

Materials and Methods: In this experimental study, aerial parts of Ballota platyloma were collected from Veresk (Mazandaran, Iran). Dried aerial parts of Ballota platyloma were extracted by percolation, and polyphenol fraction. The antioxidant activity of extracts was investigated using different methods: 1, 1-diphenyl- 2-picryl hydrazyl (DPPH), iron ion chelating activity and reducing power. Data were analyzed using ANOVA and Duncan test at a significant level of P<0.05.

Results: The total phenolic and total flavonoid contents were in order of polyphenol> ultrasonic > percolation,  The IC50 for polyphenol, percolation and ultrasonic extracts were 7.52±1.07, 6.64±0.87 and 2.58±0.06, respectively. The IC50 for iron chelating ability were in order of polyphenol < ultrasonic < percolation. Polyphenol fraction showed better reducing power than the ultrasonic and percolation extracts (P<0.001) which was comparable to that of Vitamin C (P<0.05).

Conclusion: The results showed that polyphenol fraction was rich in total phenolic and total flavonoid contents. Polyphenol fraction showed the best activity in reducing power but ultrasonic extracts was the most potent one in DPPH radical-scavenging activity. Thus, antioxidant activity may be attributed to the presence of phenols and flavonoids in the extracts.

Keywords: Antioxidant activity, Ballota platyloma, Flavoniods, Ultrasonic method, Polyphenol fraction
Full-Text [PDF 335 kb]   (7282 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/06/19 | Revised: 2017/08/7 | Accepted: 2016/06/19 | Published: 2016/06/19
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Seyedalipour B, Hasani A, Ebrahimzadeh M A, Taravati A. Antioxidant activity, total flavonoid and total phenolic contents of extracts taken from aerial parts of Ballota platyloma using three different methods: percolation, ultrasonic and polyphenolic fraction. Feyz 2016; 20 (2) :147-156
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