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:: Volume 20, Issue 2 (Bimonthly 2016) ::
Feyz 2016, 20(2): 133-140 Back to browse issues page
Influence of alpha-lipoic acid on survival and proliferation of mesenchymal stem cells
Tahereh Bashiri , Mohammad Taghi Ghorbanian * , Saeed Zavareh
School of Biology and Institute of Biological Sciences, Damghan University, Damghan, I. R. Iran. , ghorbanian@du.ac.ir
Abstract:   (4530 Views)

Background: Mesenchymal stem cells (MSCs) contribute to tissue repair in vivo and form an attractive cell source for tissue engineering. The regenerative potential of MSCs is impaired by oxidative stress-induced cellular senescence. Alpha-lipoic acid (ALA) is well-known for its antioxidant properties. The Ki-67 antigen is expressed during all phases of cell cycle (G1, S, G2 and M phase) except for G0 phase and is commonly used as a proliferation marker. Herein, the aim of the present study was to investigate the impact of ALA on rat MSCs survival and proliferative potential in vitro.

Materials and Methods: Isolated rat bone marrow and derived mesenchymal stem cells were synchronized by serum starvation for 24h and the addition of hydroxyurea (2µM). Afterwards, the cells were cultured in the presence of ALA (1µM) for 48h. An MTT assay was used to investigate cell survival and proliferation. The expression of Ki-67, a proliferation marker, was also evaluated.

Results: The MMT assay showed a statistically significant increase in proliferation of MSCs in ALA-treated groups for 48 hours. Immunoctytochemistry of Ki-67 revealed significant differences between ALA- treated and Control groups.

Conclusion: In conclusion, ALA is effective in increasing the survival and cell proliferation of isolated rat bone marrow and derived mesenchymal stem cells.

Keywords: Mesenchymal Stem Cells, Proliferation, Alpha Lipoic Acid, Ki-67 protein
Full-Text [PDF 389 kb]   (1712 Downloads)    
Type of Study: Research | Subject: medicine, paraclinic
Received: 2016/06/18 | Revised: 2017/08/1 | Accepted: 2016/06/18 | Published: 2016/06/18
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Bashiri T, Ghorbanian M T, Zavareh S. Influence of alpha-lipoic acid on survival and proliferation of mesenchymal stem cells . Feyz 2016; 20 (2) :133-140
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Volume 20, Issue 2 (Bimonthly 2016) Back to browse issues page
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