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The effect of eight weeks of combined training and taurine supplementation on the expression of SATA3 and ANGPT2 genes in the kidney tissue of male wistar rats with type 2 diabetes
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Belal Mahdavi *    , Roghayeh Afroundeh , Ameneh Ourrahim , Abolfazl Bayrami |
| mohaghegh ardabili university , mahdavibelal1984@chmail.ir |
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Abstract: (6 Views) |
Background& purpose: Diabetic nephropathy (DN) is a common complication of type 2 diabetes, and physical activity along with proper nutrition may help control its associated inflammation. The present study aimed to investigate the effects of eight weeks of combined training and taurine supplementation on the gene expression of STAT3 and ANGPT2 in the kidney tissue of diabetic male rats.
Method: In this experimental study,50 eight-week-old male Wistar rats were randomly assigned into five equal groups: healthy control, diabetic control, diabetic supplement, diabetic training, and diabetic training+supplement. Diabetes was induced by intraperitoneal injection of streptozotocin. The combined training program (resistance and endurance) was performed for eight weeks, five sessions per week. Resistance training started with three sets of four repetitions and progressed to three sets of five repetitions using weights equivalent to 3% of body weight. Endurance training began at 15 m/min for 15 minutes and increased to 20 m/min for 40 minutes. Taurine supplementation was administered intraperitoneally at a dose of 20 mg/kg after exercise sessions. 48hours after the final session, kidney tissues were collected and gene expression was analyzed using Real-Time PCR. Data were analyzed using ANOVA at a significance level of p≤0.05.
Results: A significant between-group difference was observed in the renal mRNA expression of STAT3 (FWelch=21.533, p=0.001, η2=0.677) and ANGPT2 (F(4,45)=26.883, p=0.001, η2=0.705). Post-hoc analysis revealed that renal STAT3 gene expression in the diabetic exercise + supplement group (2.98±0.94) was significantly lower than that in the diabetic control group (4.30±0.34, p=0.011). Additionally, ANGPT2 expression was significantly decreased in the diabetic exercise + supplement group (1.98±0.26) compared with the diabetic control group (2.92±0.13, p=0.001).
Conclusion: Overall, exercise and supplementation appear to exert additive effects in downregulating the deleterious expression of STAT3 and ANGPT2 genes, representing an effective, non-pharmacological strategy for protecting the kidneys against diabetic complications.
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| Keywords: Diabetes, Combined exercise, Taurine supplementation, Inflammation, Signal transducer and activator of transcription 3, Angiopoietin-2. |
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Type of Study: Research |
Subject:
General
Received: 2026/04/8 | Revised: 2026/10/13 | Accepted: 2026/10/13
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