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:: Volume 29, Issue 5 (Bimothly 2025) ::
Feyz Med Sci J 2025, 29(5): 447-456 Back to browse issues page
Evaluation of the cytotoxic effects of diclofenac on pancreatic cancer cells (PANC-1) compared to non-cancerous embryonic kidney cells (HEK293)
Yasam Aliyan , Rahim Ahmadi , Tahereh Ghaderi Barmi , Sajedeh Asrar , Nooshin Amini *
Member Global Research, Education, and Event Network (GREEN), Iran , amini.n.2486@gmail.com
Abstract:   (318 Views)
Background and Aim: Pancreatic ductal adenocarcinoma is among the most lethal gastrointestinal malignancies, characterized by a poor prognosis. This study was designed to evaluate the cytotoxic effect of diclofenac on the pancreatic cancer cell line (PANC-1) and to determine its selective potential in comparison with non-cancerous HEK293 cells.
Methods: In this experimental study, the dose-dependent effects of diclofenac (31.25-500 µg/mL) on the viability of PANC-1 and HEK293 cells, following 48 hours of drug exposure, were assessed using the MTT assay. The half-maximal inhibitory concentration (IC₅₀) for each cell line was calculated using dose-response curves and GraphPad Prism software. Statistical analysis of the data was performed using one-way ANOVA followed by Tukey's post-hoc test.
Results: Diclofenac, at concentrations ranging from 31.25 to 500 µg/mL, induced a significant reduction in cell viability in both PANC-1 and HEK293 cell lines (P < 0.001). The IC₅₀ value of the drug was determined to be 96 µg/mL for the PANC-1 cell line and 107 µg/mL for the HEK293 cell line, indicating a notable cytotoxic effect of the drug on PANC-1 cells.
Conclusion: Diclofenac exhibits cytotoxic and potential anticancer effects on pancreatic cells and could be considered as a complementary option in the development of novel therapeutic strategies for this cancer. However, further pre-clinical and clinical studies are essential to fully evaluate its efficacy and safety profile.
Keywords: Pancreatic cancer, Diclofenac, Cytotoxic effect, PANC-1 Cell Line, HEK293 Cell Line
     
Type of Study: Research | Subject: General
Received: 2025/08/10 | Revised: 2025/11/2 | Accepted: 2025/09/14 | Published: 2025/11/3
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Aliyan Y, Ahmadi R, Ghaderi Barmi T, Asrar S, Amini N. Evaluation of the cytotoxic effects of diclofenac on pancreatic cancer cells (PANC-1) compared to non-cancerous embryonic kidney cells (HEK293). Feyz Med Sci J 2025; 29 (5) :447-456
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Volume 29, Issue 5 (Bimothly 2025) Back to browse issues page
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