The Effect of Dexmedetomidine on Oxidative Stress in Kidney Tissue of Male Mice Subjected to Chronic Restraint Stress

Ebrahimi Masouleh, Tahereh; Rahmani Kahnamooei, Jafar; Nasirzadeh, Mohammadreza

doi:10.48307/FMSJ.2026.30.4.393

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Volume 30, Issue 4 (Bimonthly 2026)

Feyz Med Sci J 2026, 30(4): 393-403

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The Effect of Dexmedetomidine on Oxidative Stress in Kidney Tissue of Male Mice Subjected to Chronic Restraint Stress

Tahereh Ebrahimi Masouleh

, Jafar Rahmani Kahnamooei ^*

, Mohammadreza Nasirzadeh

Department of Clinical sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran , rahmani@iau.ir

Abstract: (175 Views)

Background and Aim: Anxiety and depression are important health diseases in today's societies. Chronic restraint stress disrupts the oxidant–antioxidant balance. Dexmedetomidine (Dex) is an α2-adrenergic receptor agonist used as a preanesthetic drug. This study aimed to investigate the protective effects of dexmedetomidine (Dex) against oxidative stress induced by chronic restraint stress in mouse kidney tissue.
Methods: In this experimental interventional study, 40 laboratory mice were divided into five groups (n=8): 1) Control, 2) Normal Saline (NS)-Stress, 3) Dex20-Stress, 4) Dex40-Stress, and 5) Dex80-Stress. The mice were subjected to a chronic restraint stress model for 21 days, 3 hours daily. Animals in the Dex groups received the drug at doses of 20, 40, and 80 µg/kg during the stress induction period. At the end of the study, serum levels of corticosterone, urea, and creatinine, as well as the levels of antioxidant enzymes in kidney tissue were measured.
Results: Chronic restraint stress significantly increased serum levels of corticosterone, urea, and creatinine compared with the control group (P<0.05). Dexmedetomidine reduced serum corticosterone levels from 283.20 ng/mL in the stress group to 80.70 ng/mL in the Dex-80 group (P<0.05). In addition, stress significantly increased the level of the lipid peroxidation index and decreased the activities of the antioxidant enzymes SOD and GPX. Administration of dexmedetomidine restored the levels of corticosterone and the activities of the antioxidant enzymes SOD and GPX toward those observed in the control group (P<0.05).
Conclusion: The findings indicate that dexmedetomidine has antioxidant properties and can attenuate stress-induced changes in serum urea, creatinine, and corticosterone levels, as well as MDA levels and the activities of the antioxidant enzymes SOD and GPX in the kidney tissue of male mice subjected to chronic restraint stress.

Keywords: Chronic restraint stress, Oxidative stress, Dexmedetomidin

Full-Text [PDF 823 kb] (28 Downloads)

Type of Study: Research | Subject: medicine, paraclinic
Received: 2025/07/27 | Revised: 2026/10/6 | Accepted: 2026/09/20 | Published: 2026/10/4

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Ebrahimi Masouleh T, Rahmani Kahnamooei J, Nasirzadeh M. The Effect of Dexmedetomidine on Oxidative Stress in Kidney Tissue of Male Mice Subjected to Chronic Restraint Stress. Feyz Med Sci J 2026; 30 (4) :393-403
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