The effects of eight weeks of high-intensity interval training combined with curcumin supplementation on cardiac tissue levels of Galectin-3 and Peroxiredoxin 4 in doxorubicin-treated rats with breast cancer

Laghaei, Narges; Hosseinpour Delavar, Sedighe; Ghahramani, Mehran

doi:10.48307/FMSJ.2025.29.5.436

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Volume 29, Issue 5 (Bimothly 2025)

Feyz Med Sci J 2025, 29(5): 436-446

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The effects of eight weeks of high-intensity interval training combined with curcumin supplementation on cardiac tissue levels of Galectin-3 and Peroxiredoxin 4 in doxorubicin-treated rats with breast cancer

Narges Laghaei

, Sedighe Hosseinpour Delavar ^*

, Mehran Ghahramani

Department of Exercise Physiology, Ker.C., Islamic Azad University, Kermanshah, Iran , delavar@iau.ac.ir

Abstract: (262 Views)

Background and Aim: Chemotherapy with agents such as doxorubicin and trastuzumab in breast cancer patients can lead to cardiotoxicity. Understanding the mechanisms underlying this damage is crucial for mitigating adverse effects and supporting the continuation of effective therapy. The present study aimed to evaluate the effects of high-intensity interval training (HIIT) and curcumin supplementation on the cardiac tissue levels of Galectin-3 and Peroxiredoxin 4 in doxorubicin-treated rats with breast cancer.
Methods: In this experimental study, 40 female Wistar rats (18-19 g initial weight) were allocated into five groups: 1) Cancer + Doxorubicin, 2) Cancer without treatment, 3) Cancer + Doxorubicin + Exercise, 4) Cancer + Doxorubicin + Curcumin, 5) Cancer + Doxorubicin + Exercise + Curcumin. The HIIT protocol consisted of six intervals at 80–95% of VO₂max with active recovery at 30–35% of VO₂max, conducted over eight weeks, five days per week. Curcumin was administered via gavage at a dose of 100 mg/kg, one-hour post-exercise.
Results: Cardiac Galectin-3 levels were significantly elevated in the doxorubicin group, whereas Peroxiredoxin 4 levels were markedly reduced (P=0.001). In contrast, the group receiving the combined intervention of HIIT and curcumin demonstrated a significant decrease in Galectin-3 and a significant increase in Peroxiredoxin 4 levels (P=0.001). Although both HIIT and curcumin interventions alone produced similar directional effects, the combined effect was significantly more effective.
Conclusion: The combination of high-intensity interval training and curcumin supplementation appears to confer significant protective effects against doxorubicin-induced cardiotoxicity, potentially through the modulation of inflammatory and oxidative stress markers. This combined approach may represent a promising strategy for mitigating the cardiac complications associated with chemotherapy.

Keywords: Breast cancer, Doxorubicin, Curcumin, High-intensity interval training, Galectin-3

Type of Study: Research | Subject: General
Received: 2025/07/20 | Revised: 2025/11/2 | Accepted: 2025/10/12 | Published: 2025/11/3

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‎ 10.48307/FMSJ.2025.29.5.436

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Laghaei N, Hosseinpour Delavar S, Ghahramani M. The effects of eight weeks of high-intensity interval training combined with curcumin supplementation on cardiac tissue levels of Galectin-3 and Peroxiredoxin 4 in doxorubicin-treated rats with breast cancer. Feyz Med Sci J 2025; 29 (5) :436-446
URL: http://feyz.kaums.ac.ir/article-1-5377-en.html

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