:: Volume 26, Issue 6 (Bimonthly 2022) ::
Feyz 2022, 26(6): 683-690 Back to browse issues page
Interactive effects of aerobic training and Crocin supplementation on some apoptosis markers in kidney tissue of rats exposed to doxorubicin
Firouzeh Maskanati , Seyedeh Ommolbanin Ghasemian , Rahmatollah Khanmohammadi , Omidreza Salehi
Department of Veterinary, Behbahan Branch, Islamic Azad University, Behbahan, I.R. Iran. , ghasemian1249@yahoo.com
Abstract:   (779 Views)
Background: The use of doxorubicin for therapeutic purposes is associated with the occurrence of kidney disorders. However, this study aimed to investigate the interactive effects of aerobic training and Crocin supplementation on B-lymphocyte lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) in kidney tissue of rats exposed to doxorubicin.
Materials and Methods: 40 male Wistar rats (age 8-10 weeks, weight 200-220 grams) were randomly divided to groups: (1) healthy control, (2) doxorubicin (2 mg/kg/day), (3) doxorubicin+training, (4) doxorubicin+crocin and (5) doxorubicin+exercise+crocin. Aerobic training was performed for eight weeks, three sessions a week (with an intensity of 60-70% of VO2max); and crocin was gavaged 50 mg/kg/day. To analyze the data, one-way analysis of variance with Tukey's post hoc test was used (P≥0.05).
Results: Bax ​​and Bax/BCL2 ratio were lower in doxorubicin+crocin and doxorubicin+exercise+crocin groups and BCL2 values ​​were higher than doxorubicin group (P=0.001). Bax ​​and Bax/BCL2 ratio in doxorubicin+exercise group were lower than doxorubicin group (P=0.001). Bax ​​and Bax/BCL2 ratio were lower in doxorubicin+exercise+crocin group and BCL2 ​​was higher in this group than doxorubicin+crocin group (P=0.001). BCL2 ​​ in the doxorubicin+exercise+crocin group was higher and the Bax/BCL2 ratio in this group was lower than the doxorubicin+exercise group (P=0.001).
Conclusion: It seems that aerobic training with reduction of Bax and Crocin with increase of BCL2 have favorable effects on the inhibition of apoptosis in the kidney tissues of rats. But these two interventions have an interactive effect in inhibiting the apoptosis of kidney tissue following exposure to doxorubicin.
Keywords: Exercise training, Crocin, Apoptosis, Kidney, Doxorubicin, Rats
Full-Text [PDF 380 kb]   (447 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/10/2 | Revised: 2023/02/22 | Accepted: 2022/12/17 | Published: 2023/02/22
References
1. Afsar T, Razak S, Almajwal A, Al-Disi D. Doxorubicin-induced alterations in kidney functioning, oxidative stress, DNA damage, and renal tissue morphology; Improvement by Acacia hydaspica tannin-rich ethyl acetate fraction. Saudi J Biol Sci 2020; 27(9): 2251–60.
2. Ayla S, Seckin I, Tanriverdi G, Cengiz M, Eser M, Soner BC, et al. Doxorubicin induced nephrotoxicity: protective effect of nicotinamide. Int J Cell Biol 2011; 2011.
3. AlAsmari AF, Ali N, Alharbi M, Alqahtani F, Alasmari F, Almoqbel D, et al. Geraniol Ameliorates Doxorubicin-Mediated Kidney Injury through Alteration of Antioxidant Status, Inflammation, and Apoptosis: Potential Roles of NF-κB and Nrf2/Ho-1. Nutrients 2022; 14(8): 1620.
4. Eydivandi K, Azarbayjani MA, Farzanegi P. The Effect of High Intensity Interval Training (HIIT) and Crocin Consumption on Doxorubicin-Induced Apoptotic Indices in the Muscle Tissue of Rats. Gene, Cell Tissue 2020;7(4).
5. Farzanegi P, Abbaszadeh H, Farokhi F, Rahmati-Ahmadabad S, Hosseini SA, et al. Attenuated renal and hepatic cells apoptosis following swimming exercise supplemented with garlic extract in old rats. Clin Interv Aging 2020; 15: 1409.
6. Momeni L, Moghadam HF, Hosseini SA, Nikbakht M. Interactive Effects of Endurance Training and Selenium Consumption on the Intrinsic Apoptosis Pathway in the Liver Tissue of Cadmium-Exposed Rats. J Nutr Sci Diet 2019; 1-10.
7. Davari F, Alimanesh Z, Alimanesh Z, Salehi O, Hosseini SA. Effect of training and crocin supplementation on mitochondrial biogenesis and redox-sensitive transcription factors in liver tissue of type 2 diabetic rats. Arch Physiol Biochem 2020; 1–6.
8. Hosseini SA, Norouzi S, Rafiee N, Farzanegi P, Salehi O, Farkhaie F. Interactive Effects of Endurance Training and Crocin on Aerobic Capacity, Dietary Intake and Weight of High-Fat Diet-Induced Type 2 Diabetic Rats. J Nutr Sci Diet 2018; 4(3): 65-74.
9. El-Ashmawy NE, Khedr EG, Doghish AS, Elballal MS. Carnosine and crocin ameliorate oxidative stress in rats with rhabdomyolysis-induced acute kidney injury through upregulating HO-1 gene expression. Food Biosci 2022;101972.
10. Keelo RMAH, Elbe H, Bicer Y, Yigitturk G, Koca O, Karayakali M, et al. Treatment with crocin suppresses diabetic nephropathy progression via modulating TGF-β1 and oxidative stress in an experimental model of pinealectomized diabetic rats. Chem Biol Interact 2022; 351: 109733.
11. Davoodi M, Bouri SZ, Ghahfarokhi SD. Antioxidant effects of aerobic training and crocin consumption on doxorubicin-induced testicular toxicity in rats. J Fam Reprod Heal 2021; 15(1): 28.
12. Moradi M, Shakerian S, Nikbakht M. Effect of eight weeks of high intensity interval training and crocin consumption on the apoptotic genes expression in the liver tissue of male rats under chronic doxorubicin induction. J Birjand Univ Med Sci 2020; 27(4): 323–35. [in Persian]
13. Ruggiero A, Ferrara P, Attinà G, Rizzo D, Riccardi R. Renal toxicity and chemotherapy in children with cancer. Br J Clin Pharmacol 2017; 83(12): 2605–14.
14. Yang L, Li D, Cao B, Liu S, Xu D, Zhu X, et al. Exercise training ameliorates early diabetic kidney injury by regulating the H2S/SIRT1/p53 pathway. FASEB J 2021;35(9):e21823.
15. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene, Cell Tissue 2015; 2(4): 6-12.
16. Yazdanparast Chaharmahali B, Azarbayjani MA, Peeri M, Farzanegi Arkhazloo P. Increased Expression of Bax and Bcl2 Apoptosis Biomarkers in the Heart of Old Female Rats After Interval Training and Curcumin Consumption. Jorjani Biomed J 2018; 6(4): 40–52.
17. Ghazal A, Raheleh R, Majid RN. Effect of Crocin on Bax, Bcl2, and Oxidative Stress Indices in Testicular Tissue of Streptozotocin Diabetic Rats. Ofogh-e-Danesh 2019; 25: 340–51. [in Persian]
18. Shadegan PA, Khajehlandi A, Mohammadi A. Effect of Aerobic Training and Crocin Consumption on Bax Gene Expression in the Hippocampal Tissue of Ovariectomized Rats. Gene, Cell Tissue 2020; 7(3): 4-9.



XML   Persian Abstract   Print



Creative Commons License
This open access journal is licensed under a Creative Commons Attribution-NonCommercial ۴.۰ International License. CC BY-NC ۴. Design and publishing by Kashan University of Medical Sciences.
Copyright ۲۰۲۳© Feyz Medical Sciences Journal. All rights reserved.
Volume 26, Issue 6 (Bimonthly 2022) Back to browse issues page