:: Volume 23, Issue 5 (Bimonthly 2019) ::
Feyz 2019, 23(5): 528-534 Back to browse issues page
Antibacterial activity of zinc oxide nanoparticles on standard strains and isolates of Escherichia coli and Listeria monocytogenes from food
Zahra Dargahi , Ali Reza Partoazar , Shima Naddafi , Mohammad Mehdi Soltan-Dallal
Food Microbiology Research Center, School of Public Health, Tehran University of Medical Sciences, Tehran, I.R. Iran. , msoltandallal@gmail.com
Abstract:   (2318 Views)
Background: Due to the human need for food, any change in the quality and quantity of food will affect the health of the community. It is important to remove microbial contaminants from food during the production, storage and supply phases. In this study, the antibacterial effects of nanoparticles of zinc oxide on two Escherichia coli and Listeria monocytogenes were investigated.
Materials and Methods: In this study, zinc oxide nanoparticles were prepared from zeolite and quantified by X-ray fluorescence. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of nanoparticles of zinc oxide were determined using a disc diffusion method. Graphpad prism statistical software was used for data analysis and ANOVA was used for analysis of variance. Significant limit was set at 0.05 (P≤0.05).
Results: Based on the results of this study, the MIC value of nanoparticles of zinc oxide for all tested bacteria was 4 mg/ml, and the MBC values for standard strain and Escherichia coli isolates were 8 and 4 mg/ml, respectively. The standard strain and isolate of Listeria monocytogenes was calculated to be 4 mg/ml.
Conclusion: The present study showed that zinc oxide nanoparticles can be used as a deterrent against the pathogens of the materials and avoid contamination.
Keywords: Nanoparticle, Zinc Oxide, Escherichia coli, Listeria monocytogenes, Antibacterial
Full-Text [PDF 279 kb]   (1265 Downloads)    
Type of Study: Research | Subject: General
Received: 2019/05/30 | Revised: 2019/12/23 | Accepted: 2019/10/5 | Published: 2019/12/16
References
1. Bahmanabadi R, Khalili MB, Soltan Dallal MM. The Study of Enteropathogenic Escherichia Coli Prevalence by PCR Method in Under-5-Year-Old Children’s Diarrheal Samples Caused by the Country’s Food. Payavard-e-Salamat 2017; 11(6): 715-22. [in Persian]
2. Murphy HM, Payne SJ, Gagnon GA. Sequential UV-and chlorine-based disinfection to mitigate Escherichia coli in drinking water biofilms. Water Res 2008; 42(8-9): 2083-92.
3. Tosa K, Hirata T. Photoreactivation of enterohemorrhagic Escherichia coli following UV disinfection. Water Res 1999; 33(2): 361-6.
4. Hof H, Nichterlein T, Lampidis R, Wecke J. Listeria dispose of many facettes. Biotest Bull 1998; 6: 21-3.
5. Aguado V, Vitas AL, Garcia-Jalon I. characterization of listeria monocytogenes and listeria innocua from a vegetable processing plant by RAPD and REA. Int J Food Microbial 2004; 90(3): 341-7.
6. Hoseinzadeh E, Samargandi MR, Alikhani MY, Roshanaei GH, Asgari GH. Antimicrobial Efficacy of Zinc Oxide Nanoparticles Suspension Against Gram Negative and Gram Positive Bacteria. Iran J Health Environ 2012; 5(3): 463-74. [in Persian]
7. Caratto V, Ball L, Sanguineti E, Insorsi A, Firpo I, Alberti S, et al. Antibacterial activity of standard and N-doped titanium dioxide-coated endotracheal tubes: an in vitro study. Rev Bras Ter Intensiva 2017; 29(1): 55-62
8. Kolodziejczak A, Jesionowski T. Zinc oxide from synthesis to application. Materials 2014; 7(4): 2833-81.
9. Ketabchi M, Iessazadeh Kh, Massiha A. Evaluate the inhibitory activity of ZnO nanoparticles against standard strains and isolates of Staphylococcus aureus and Escherichia coli isolated from food samples. JFM 2017; 4(1): 63-74.
10. Sawai J, Yoshikawa T. Quantitative evaluation of antifungal activity of metallic oxide powders (MgO, CaO and ZnO) by an indirect conductimetric assay. J Appl Microbiol 2004; 96: 803-9.
11. Makhluf S, Dror R, Nitzan Y, Abramovich Y, Jelinek R, Gedanken A. Microwave-Assisted Synthesis of Nanocrystalline MgO and Its Use as a Bacteriocide. Adv Funct Mater 2005; 15: 1708-15.
12. Alswat AA, Ahmad MB, Saleh TA, Hussein MZB, Ibrahim NA. Effect of zinc oxide amounts on the properties and antibacterial activities of zeolite/zinc oxide nanocomposite. Mater Sci Eng C Mater Biol Appl 2016; 68: 505-11.
13. Azam A, Ahmed AS, Oves M, Khan MS, Habib SS, Memic A. Antimicrobial activity of metal oxide nanoparticles against Gram-positive and Gram-negative bacteria: a comparative study. Int J Nanomedicine 2012; 7: 6003.
14. Mohammadbeigi P, Sodagar M, Mazandarani M, Hoseini Ss. An Investigation of Antibacterial Activity of Zno Nanoparticle on Streptoccocus Iniae and Escheria Coli. Qom Univ Med Sci J 2016; 10(5): 55-63. [in Persian]
15. Gunalan S, Sivaraj R, Rajendran V. Green synthesized ZnO nanoparticles against bacterial and fungal pathogens, Progress in Natural Science. Materials Int 2012; 22(6): 695–702.
16. Parish M. Orange juice quality after treatment by ZnO nanoparticle or thermal pasteurization isostatic high pressure, 2011. Lwt 1998; 31: 439-42.
17. Adams LK, Lyon DY, Alvarez PJJ. Comparative ecotoxicity of nanoscale TiO2, SiO2, and ZnO water suspensions. Watter Res 2006; 40: 3527-32.
18. Hosseini SS, Mohammadi SH, Joshagani HR, Eskandari M. Colorimetric MTT assessment of antifungal activity of ZnO nanowires against candida dubliensis bioflm. Jondishapur Med Sci 2013; 12(1): 69-80. [in Persian]
19. Zhang H, Chen G. Potent antimicrobial activity of Ag/TiO2 nanocomposite powder synthesized by a one-pot sol-gel method. Environ. Sci Technol 2009; 43: 2905–10.
20. Sinha R, Karan R, Sinha A, Khare SK. Interaction and nanotoxic effect of ZnO and Ag nanoparticles on mesophilic and halophilic bacterial cells. Bioresour Technol 2011; 2: 1516-20.
21. Reddy KM, Feris K, Bell J, Wingett DG, Hanley C, Punnoose A. Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systems. Appl Phys Lett 2007; 90(213902): 2139021-3.
22. Gunalan S, Sivaraj R, Rajendran V. Green synthesized ZnO nanoparticles against bacterial and fungal pathogens, Progress in Natural Science. Materials Intl 2012; 22(6): 695–702.
23. Ramani M, Ponnusamy S, Muthamizhchelvan C. From zinc oxide nanoparticles to microflowers: A study of growth kinetics and biocidal activity. Materials Sci Engineering 2012; 32(8): 2381-89.
24. Seil JT, Webster TJ. Reduced Staphylococcus aureus proliferation and biofilm formation on zinc oxide, nanoparticle PVC composite surfaces. Acta Biomater 2011; 7(6): 2579-84.
25. Ma J, Liu J, Bao Y, Zhu Z, Wang X, Zhang J. Synthesis of large-scale uniform mulberry-like ZnO particles with microwave hydrothermal method and its antibacterial property. Ceramics Int 2013; 39(3): 2803-10.
26. Liu Y, He L, Mustapha A, Li H, Hu Z, Lin M. Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157: H7. J Applied Microbiol 2009; 107(4): 1193-201.
27. Handy R, von der Kammer F, Lead J, Hassellov M, Owen R, Crane M. The ecotoxicology and chemistry of manufactured Nanoparticles. Ecotoxicology 2008; 17(4): 287-314.
28. Ostrowski AD, Martin T, Conti J, Hurt I, Harthorn BH. Nanotoxicology: Characterizing the scientific literature, 2000-2007. J Nanopart Res 2009; 11(2): 251-57.

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