[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: ::
Back to the articles list Back to browse issues page
Effect of chronic caffeine administration on hyperalgesia in a rat neuropathic pain model: role of nitric oxide pathway
Monir Naderi Tehrani , GHolamali Hamidi * , Saeedeh Nasrollahi , Azhdar Heydari
Physiology Research Center, Institute for Basic Sciences, Kashan University of Medical Sciences, Kashan, I.R. Iran. , hamiidi@yahoo.com
Abstract:   (203 Views)
Background: Neuropathic pain is a chronic pain caused by damage to the central nervous system and the peripheral. Caffeine is a non-selective antagonist of A1, A2a, receptors of adenosine, which has a protective effect on neuropathic pain in some doses by inhibiting A2a, A2b receptors. Considering that the nitric oxide (NO) levels are apparently effective in the parts of caffeine central effects, thus, the purpose of this study was to investigate the effect of chronic caffeine administration on the hyperalgesia in neuropathic rats and levels of nitric oxide metabolites (NOx).
Materials and Methods: The present study was conducted on 40 adult male rats weighing 220-250 grams. Neuropathic pain was induced by chronic constriction injury (CCI) of sciatic nerve.  Animals were randomly divided into 5 groups (n=8). The control group, which did not intervene on the sciatic nerve, The sham group, which the animals were surgically implanted but the sciatic nerve was not tied, The CCI group and test groups received oral doses of  caffeine orally (100 and 300 mg/L) for 28 days. Hyperalgesia was measured in all groups with Plantar test on days 4, 7, 14, 21, and 28 after surgery. NOx were measured with the Griess method in lumbar spinal cord tissue on day 28.
Results: Neuropathic rats showed decreased pain thresholds in hyperalgesia. Chronic caffeine at the doses of 100 and 300 mg/L in drinking water for 28 days significantly alleviated hyperalgesia (P<0.01, P<0.001).
Conclusion: The results of this study showed that chronic intake of caffeine can reduce hyperalgesia in neuropathic rat. It seems that NO pathway is not involved in the central effect of caffeine on pain threshold in CCI model of neuropathic pain.
Keywords: Neuropathic pain, Caffeine, Nitric oxide, Hyperalgesia
     
Type of Study: Research | Subject: medicine, paraclinic
Received: 2019/02/6 | Accepted: 2019/05/18
References
1. Alles SRA, Smith PA. Etiology and pharmacology of neuropathic pain. Pharmacol Rev 2018; 70(2): 315-47.
2. Luszczki JJ, Zuchora M, Sawicka KM, Kozińska J, Czuczwar SJ. Acute exposure to caffeine decreases the anticonvulsant action of ethosuximide, but not that of clonazepam, phenobarbital and valproate against pentetrazole-induced seizures in mice. Pharmacol Rep 2006; 58(5): 652-9.
3. Bernstein GA, Carroll ME, Thuras PD, Cosgrove KP, Roth ME. Caffeine dependence in teenagers. Drug Alcohol Depend 2002; 66(1): 1-6.
4. Tavares C, Sakata RK. Caffeine in the treatment of pain. Rev Bras Anestesiol 2012; 62(3): 394-401.
5. Ferré S, Orrú M, Guitart X. Paraxanthine: Connecting caffeine to nitric oxide neurotransmission. J Caffeine Res 2013; 3(2): 72-8.
6. Sawynok J. Adenosine receptor targets for pain. Neuroscience 2016; 338: 1-18.
7. El Yacoubi M, Ledent C, Parmentier M, Bertorelli R, Ongini E, Costentin J, Vaugeois JM. Adenosine A2A receptor antagonists are potential antidepressants: evidence based on pharmacology and A2A receptor knockout mice. Br J Pharmacol 2001; 134(1): 68-77.
8. Sawynok J. Adenosine receptor activation and nociception. Eur J Pharmacol 1998; 347(1): 1-11.
9. Wu WP, Hao JX, Fredholm BB, Wiesenfeld-Hallin Z, Xu XJ. Effect of acute and chronic administration of caffeine on pain-like behaviors in rats with partial sciatic nerve injury. Neurosci Lett 2006; 402(1-2): 164-6.
10. Bruce C, Yates DH, Thomas PS. Caffeine decreases exhaled nitric oxide. Thorax 2002; 57(4): 361-3.
11. Cury Y, Picolo G, Gutierrez VP, Ferreira SH. Pain and analgesia: The dual effect of nitric oxide in the nociceptive system. Nitric Oxide 2011; 25(3): 243-54.
12. Yan YY, Li CY, Zhou L, Ao LY, Fang WR, Li YM. Research progress of mechanisms and drug therapy for neuropathic pain. Life Sci 2017; 190: 68-77.
13. Boison D. Methylxanthines, seizures, and excitotoxicity. Handb Exp Pharmacol 2011; (200): 251-66.
14. Banafsheh HR, Hajhashemi V, Minaiyan M, Mesdaghinia A ,Abed A. Antinociceptive effects of maprotiline in a rat model of peripheral neuropathic pain: possible involvement of opioid system. Iran J Basic Med Sci 2015; 18(8): 752.
15. Banafsheh H, Mesdaghinia A, Honarkar-Ramezani M, Noorani-Arani M, Banitaba-Bidgoli, Hamidi GA. Effect of lithium on neuropathic pain induced by partial ligation of rat sciatic nerve. Feyz 2012; 15(4): 294-301. [in Persian]
16. Wen J, Sun D, Tan J, Young W. A consistent, quantifiable, and graded rat lumbosacral spinal cord injury model. J Neurotrauma 2015; 32(12): 875-92.
17. Heydari A, Davoudi S. The effect of sertraline and 8-OH-DPAT on the PTZ_induced seizure threshold: Role of the nitrergic system. Seizure 2017; 45: 119-24.
18. Mareš P. A1 not A2A adenosine receptors play a role in cortical epileptic afterdischarges in immature rats. J Neural Transm (Vienna) 2014; 121(11): 1329-36.
19. Grace PM, Gaudet AD, Staikopoulos V, Maier SF, Hutchinson MR, Salvemini D. Nitroxidative signaling mechanisms in pathological pain. Trends Neurosci 2016; 39(12): 862-79.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print



Back to the articles list Back to browse issues page
مجله علمی پژوهشی فیض ::: دانشگاه علوم پزشکی کاشان KAUMS Journal ( FEYZ )
Persian site map - English site map - Created in 0.05 seconds with 32 queries by YEKTAWEB 3921